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Phytohormones and Antibiotics Produced by Bacillus subtilis and their Effects on Seed Pathogenic Fungi and on Soybean Root Development

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Summary

Bacteria belonging to the genus Bacillus were isolated from soil samples of Paraná State, Brazil, with the aim of evaluating their potential biological control of soybean seed pathogens. Strain PRBS-1 was selected, showing similar effectiveness to that of the strain AP-3, used as a reference due to its known antibiotic potential. The sequencing of the ribosomal 16S rRNA gene confirmed that both strains belong to the species B. subtilis, although showing high genetic diversity in relation to this species. Both strains inhibited five soybean seed pathogenic fungi in vitro, Rhizoctonia solani, Colletotrichum truncatum, Sclerotinia sclerotiorum, Macrophomina phaseolina and Phomopsis sp. Furthermore, the metabolites of AP-3 increased production of root hairs, while the metabolites of PRBS-1 stimulated outgrowth of lateral roots in soybean. The antibiotic effect of both strains seemed to be related to compounds of the iturin group, while the root growth promotion by PRBS-1 was at least partially related to the production of indoleacetic acid. The results have shown the potential of using selected strains of B. subtilis in the biological control of seed pathogens, as well as in promoting soybean growth.

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References

  • J. Aldrich R. Baker (1970) ArticleTitleBiological control of Fusarium roseum f. sp. dianthi by Bacillus subtilis Plant Disease Reporter 54 446–448

    Google Scholar 

  • Araújo, F.F. & Hungria, M. 1995 Comportamento a campo e casa de vegetação de soja co-inoculada com Bacillus e Bradyrhizobium. In: Microbiologia do Solo, Desafios para o Século XXI, Hungria, M., Balota, E.L., Colozzi-Filho, A. & Andrade, D.S. (eds.), (pp. 456–461). IAPAR/EMBRAPA-CNPSo, Londrina, Brazil

  • F.F. Araújo M. Hungria (1999) ArticleTitleNodulação e rendimento de soja co-infectada com Bacillus subtilis e Bradyrhizobium japonicum/Bradyrhizobium elkanii Pesquisa Agropecuária Brasileira 34 1633–1643

    Google Scholar 

  • Araújo, F.F., Henning A., Hungria, M. & Lima, J. 1995 Caracterização do potencial antifúngico de Bacillus spp. isolados de solos do Paraná. In: Microbiologia do Solo, Desafios para o Século XXI. Hungria, M., Balota, E.L., Colozzi-Filho, A., Andrade, D.S. (eds.), (pp. 450–455). IAPAR/EMBRAPA-CNPSo, Londrina, Brazil

  • Bernal G, Illanes, A. & Ciampi, L. 2002 Isolation and partial purification of a metabolite from a mutant strain of Bacillus sp. with antibiotic activity against plant pathogenic agents. Electronic Journal of Biotechnology (on line), 15 April 2002, 5 (1). Available from http://www.ejbiotechnology.info/content/vol5/issue1/full/4/

  • F. Besson G. Michel (1987) ArticleTitleIsolation and characterization of new iturins,iturin D and iturin E Journal of Antibiotics 40 437–442 Occurrence Handle1:CAS:528:DyaL2sXktlKht74%3D

    CAS  Google Scholar 

  • F. Besson F. Peypoux G. Michel (1979) ArticleTitleAntifungal activity upon Saccharomyces cerevisiae of iturin A, mycosubtilin, bacillomycin L and of their derivatives; inhibition of this antifungal activity by lipid antagonists Journal of Antibiotics 32 828–833 Occurrence Handle1:CAS:528:DyaE1MXls12ju7o%3D

    CAS  Google Scholar 

  • W. Bettiol H. Kimati (1990) ArticleTitleEfeito do Bacillus subtilis sobre Pyricularia orizae agente causal da bruzone do arroz Pesquisa Agropecuária Brasileira 25 1165–1174

    Google Scholar 

  • W. Bettiol A. Garibaldi Q. Migueli (1997) ArticleTitleBacillus subtilis for the control of powdery mildew on cucumber and zucchini squash Bragantia 56 281–287 Occurrence Handle10.1590/S0006-87051997000200007

    Article  Google Scholar 

  • W. Bettiol M.L. Saito M.S.B. Brandão (1994) ArticleTitleControle da ferrugem do cafeeiro com produtos à base de Bacillus subtilis Summa Phytopathologica 20 119–122

    Google Scholar 

  • R.E. Buchanan N.G. Gibbons (1975) Bergey’s Manual of Determinative Bacteriology EditionNumber8 Willians & Wilkins Baltimore,

    Google Scholar 

  • L.S. Chen A. Figueredo F.O. Pedrosa M. Hungria (2000) ArticleTitleGenetic characterization of soybean rhizobia in Paraguay Applied and Environmental Microbiology 66 5099–5103 Occurrence Handle1:CAS:528:DC%2BD3cXnvFyqsbs%3D

    CAS  Google Scholar 

  • C.H. Collins P.M. Lyne (1984) Microbiological Methods EditionNumber5 Butterworths & Co London

    Google Scholar 

  • M.A. Cubeta G.L. Hartman J.B. Sinclair (1985) ArticleTitleInteraction between Bacillus subtilis and fungi associated with soybean seeds Plant Disease 69 506–509

    Google Scholar 

  • J. Dunleavy (1955) ArticleTitleControl of damping-off sugar beet by Bacillus subtilis Phytopathology 45 252–258

    Google Scholar 

  • Evans, M.L. 1984 Functions of hormones at the cellular level of organization In: Hormonal Regulation of Development II: The Functions of Hormones from the Level of the Cell to the Whole Plant v.2. Scott, T.K. (ed.), (pp. 23–62) Springer-Verlag Berlin ISBN 0–387-10196-9

  • R. Hadas Y. Okon (1987) ArticleTitleEffect of Azospirillum brasilense inoculation on root morphology and respiration in tomato seedlings Biology and Fertility of Soils 5 241–247 Occurrence Handle10.1007/BF00256908

    Article  Google Scholar 

  • L.J. Halverson J. Handelsman (1991) ArticleTitleEnhancement of soybean nodulation by Bacillus cereus UW85 in the field and in a growth chamber Applied and Environmental Microbiology 57 2767–2770 Occurrence Handle1:STN:280:DyaK387hslKhtQ%3D%3D

    CAS  Google Scholar 

  • M. Hungria G. Stacey (1997) ArticleTitleMolecular signals exchanged between host plants and rhizobia: Basic aspects and potential application in agriculture Soil Biology and Biochemistry 29 819–830 Occurrence Handle10.1016/S0038-0717(96)00239-8 Occurrence Handle1:CAS:528:DyaK2sXks1Wksrw%3D

    Article  CAS  Google Scholar 

  • Hungria, M., Campo, R.J., Mendes, I.C. & Graham, P.H. 2005 Contribution of biological nitrogen fixation to the N nutrition of grain crops in the tropics, The success of soybean (Glycine max L. Merr.) in South America, In: Nitrogen Nutrition and Sustainable Plant Productivity. Singh, R.P. & Shankar Jaiwal, N.P.K. (eds.), Studium Press, LLC, Houston, Texas (in press)

  • M. Hungria C.Y.M. Nishi J. Cohn G. Stacey (1996) ArticleTitleComparison between parental and variant soybean Bradyrhizobium strains with regard to the production of lipo-chitin nodulation signals, early stages of root infection, nodule occupancy, and N2 fixation rates Plant and Soil 186 331–341 Occurrence Handle1:CAS:528:DyaK2sXntVCktA%3D%3D

    CAS  Google Scholar 

  • W.P. Jacobs (1979) Plant Hormones and Plant Development Cambridge University Press Cambridge

    Google Scholar 

  • E. Katz A.L. Demain (1977) ArticleTitleThe peptide antibiotics of Bacillus, Chemistry, biogenesis and possible functions Bacteriology Reviews 41 449–474 Occurrence Handle1:CAS:528:DyaE2sXkvFyms78%3D

    CAS  Google Scholar 

  • M Kilian U. Steiner B. Krebs H. Junge G. Schmiedeknecht R. Hain (2000) ArticleTitleFZB24® Bacillus subtilis – mode of action of a microbial agent enhancing plant vitality Pflanzenschutz-Nachrichten Bayer 1/00/1 72–93

    Google Scholar 

  • B. Krebs H. Junge A. Ockhardt B. Hoding D. Heubner U. Erben (1993) ArticleTitleBacillus subtilis: An effective biocontrol agent Pesticide Science 37 427–429

    Google Scholar 

  • D. Li M. Alexander (1988) ArticleTitleCo-inoculation with antibiotic-producing bacteria to increase colonization and nodulation by rhizobia Plant and Soil 108 211–219

    Google Scholar 

  • D. Li M. Alexander (1990) ArticleTitleFactors affecting co-inoculation with antibiotic-producing bacteria to enhance rhizobial colonization and nodulation Plant and Soil 129 195–201

    Google Scholar 

  • Z.L. Liu J.B. Sinclair (1990) ArticleTitleEnhanced soybean plant growth and nodulation by Bradyrhizobium in the presence of strains of Bacillus megaterium Phytopathology 80 1024

    Google Scholar 

  • J.E. Loper M.N. Schroth (1986) ArticleTitleInfluence of bacterial sources of indole-3-acetic acid on root elongation of sugar beet Phytopathology 76 386–389 Occurrence Handle1:CAS:528:DyaL28XktVeltL8%3D Occurrence Handle10.1094/Phyto-76-386

    Article  CAS  Google Scholar 

  • W.C. Luz (1994) ArticleTitleEfeito da microbiolização de sementes no rendimento e controle da podridão comum das raízes e de patógenos das sementes de trigo Fitopatologia Brasileira 19 144–148

    Google Scholar 

  • R. Maget-Dana F. Peypoux (1994) ArticleTitleIturins, a special classe of pore-forming lipopeptides, Biological and physicochemical properties Toxicology 87 151–174 Occurrence Handle10.1016/0300-483X(94)90159-7 Occurrence Handle1:CAS:528:DyaK2cXivFWmsrs%3D

    Article  CAS  Google Scholar 

  • R. Maget-Dana F. Peypoux Y. imatsuno T. Ano M. Shoda (1992) ArticleTitleCloning of a gene responsible for the specific production of an antifungal antibiotic iturin with amino acid residue Journal of General and Applied Microbiology 38 505–509

    Google Scholar 

  • Y. Matsuno T. Ano M. Shoda (1992) ArticleTitleCloning of a gene responsible for the specific production of an antifungal antibiotic iturin with n-C16-ß-amino acid residue Journal of General Microbiology 38 505–509 Occurrence Handle1:CAS:528:DyaK3sXhvVClsro%3D

    CAS  Google Scholar 

  • M. Maurhofer C. Keel U. Scheneider C. Voisard D. Hass G. Defago (1992) ArticleTitleInfluence of enhanced antibiotic production in Pseudomonas fluorescens strain CHAO on its disease suppressive capacity Phytopathology 82 190–195 Occurrence Handle1:CAS:528:DyaK38XhvV2msL8%3D

    CAS  Google Scholar 

  • C.D. McKeen C.C. Reilly P.L. Pusey (1986) ArticleTitleProduction and partial characterization of antifungal substances antagonistic to Monilinia fructicola from Bacillus subtilis Phytopathology 76 136–139 Occurrence Handle1:CAS:528:DyaL28XhsVGiu70%3D

    CAS  Google Scholar 

  • Melo, I.S. 1998 Agentes microbianos de controle de fungos .topatogê nicos, In: I.S Melo, & J.L. Azevedo (Eds.), Embrapa-CNPMA, Jaguariúna.

  • P.R. Merriman R.D. Price K.F. Backer (1974) ArticleTitleThe effect of inoculation of seeds with antagonists of Rhizoctonia solani on the growth of wheat Australian Journal of Agriculture Research 25 213–218

    Google Scholar 

  • A.H. Molla Z.H. Shamsuddin M.S. Halimi M. Morziah A.B. Puteh (2001) ArticleTitlePotential for enhancement of root growth and nodulation of soybean co-inoculated with Azospirillum and Bradyrhizobium in a laboratory systems Soil Biology and Biochemistry 33 457–463 Occurrence Handle10.1016/S0038-0717(00)00186-3 Occurrence Handle1:CAS:528:DC%2BD3MXis1aksrs%3D

    Article  CAS  Google Scholar 

  • NCBI (National Center for Biotechnology Information). 2004 Retrieved on 1st March 2004, Available from http://www.ncbi.nlm.nih.gov/blast

  • C. Phae M. Shoda (1991) ArticleTitleInvestigation of optimal conditions for foam separation of iturin an antifungical peptide produced by Bacillus subtilis Journal of Fermentation and Bioengineering 71 118–121 Occurrence Handle10.1016/0922-338X(91)90235-9 Occurrence Handle1:CAS:528:DyaK3MXhsVWmsrc%3D

    Article  CAS  Google Scholar 

  • P.L. Pusey C.L. Wilson (1984) ArticleTitlePostharvest biological control of stone fruit brown rot by Bacillus subtilis Plant Disease 68 753–756

    Google Scholar 

  • G.S. Raupach J.W. Kloepper (1998) ArticleTitleMixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens Phytopathology 88 1158–1164 Occurrence Handle1:STN:280:DC%2BD1cjjvVWntw%3D%3D

    CAS  Google Scholar 

  • R. Tratch M.L.R.Z. Lima V.C. Lima Neto F.F. Araújo W. Bettiol (1993) ArticleTitleControle da podridão parda do pêssego com metabólitos de Bacillus subtilis Summa Phytopathologica 19 29–30

    Google Scholar 

  • J.T. Turner P.A. Backman (1991) ArticleTitleFactors relating to peannut yield increases following Bacillus subtilis seed treatment Plant Disease 75 347–353 Occurrence Handle10.1094/PD-75-0347

    Article  Google Scholar 

  • J.M. Vincent (1970) Manual for the Practical Study of Root Nodule Bacteria Blackwell Scientific Publications (IBP Handbook 15) Oxford, UK

    Google Scholar 

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Authors and Affiliations

  1. Faculdade de Ciências Agrárias, Universidade do Oeste Paulista, Presidente Prudente, SP, Brazil

    Fabio Fernando Araújo

  2. Embrapa Soja, Cx. Postal 23186001-970, Londrina, PR, Brazil

    Ademir Assis Henning & Mariangela Hungria

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  1. Fabio Fernando Araújo
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  2. Ademir Assis Henning
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  3. Mariangela Hungria
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Correspondence to Mariangela Hungria.

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Araújo, F.F., Henning, A.A. & Hungria, M. Phytohormones and Antibiotics Produced by Bacillus subtilis and their Effects on Seed Pathogenic Fungi and on Soybean Root Development. World J Microbiol Biotechnol 21, 1639–1645 (2005). https://doi.org/10.1007/s11274-005-3621-x

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