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Improvement in Inulinase Production by Simultaneous Action of Physical and Chemical Mutagenesis in Penicillium purpurogenum

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Summary

An extracellular inulinase (fructanase)-producing strain of Penicillium purpurogenum was isolated from the rhizosphere soil of chicory. Conidia of this selected strain were subjected to simultaneous treatment with NTG–UV (N-methyl-N′-nitro-N-nitrosoguanidine and ultraviolet radiation) and EtBr–UV (Ethidium bromide–ultraviolet radiation). After mutagenesis, colonies were screened and among them a few were selected to carry out the inulinase study, which showed a significantly higher inulinase activity with higher I/S (inulin/sucrose) ratio in all the selected colonies, indicating enhancement of inulinase production after mutagenic treatments in all the selected mutants.

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Authors and Affiliations

  1. Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Arun Dev Sharma, Prabhjot Kaur Gill, Sukhdev Singh Bhullar & Prabhjeet Singh

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  1. Arun Dev Sharma
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  2. Prabhjot Kaur Gill
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  3. Sukhdev Singh Bhullar
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  4. Prabhjeet Singh
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Correspondence to Prabhjot Kaur Gill.

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Sharma, A.D., Gill, P.K., Bhullar, S.S. et al. Improvement in Inulinase Production by Simultaneous Action of Physical and Chemical Mutagenesis in Penicillium purpurogenum . World J Microbiol Biotechnol 21, 929–932 (2005). https://doi.org/10.1007/s11274-004-6723-y

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  • DOI: https://doi.org/10.1007/s11274-004-6723-y

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