Abstract
The milk-clotting and proteolytic activities of extracellular enzyme preparations from Nocardiopsis sp. were investigated under different culture conditions. A soybean flour medium was used, with concentrations of soybean flour and of glucose varying from 0.25 to 1% w/v and from 0 to 1% w/v, respectively. Growth was monitored with 2 ml samples withdrawn from the culture medium at 8-h intervals, for determination of total protein, proteolytic activity, milk-clotting activity and sugar reduction. The best milk-clotting protease production, with a specific activity of 24.49 U/mg at 40 h, was obtained in the glucose-free medium containing soybean flour 1% w/v.
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Cavalcanti, M.T.H., Martinez, C.R., Furtado, V.C. et al. Milk-clotting protease production by Nocardiopsis sp. in an inexpensive medium. World J Microbiol Biotechnol 21, 151–154 (2005). https://doi.org/10.1007/s11274-004-3470-z
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DOI: https://doi.org/10.1007/s11274-004-3470-z