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Regulation of synthesis of endo-xylanase and β-xylosidase in Cellulomonas flavigena: a kinetic study

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Abstract

Regulation of xylanase, and β-xylosidase synthesis in Cellulomonas flavigenawas studied by culturing non-induced cells on mono-, oligo-, and poly-saccharides. The concomitant formation of these enzymes occurred on polysaccharides having structural resemblances with lignocellulosics, namely, cellulose, cellodextrin and xylan. Among disaccharides, cellobiose was the best inducer for their synthesis. Increased levels of enzymes were synthesized by the organism even under repressed conditions. Cell-free supernatants of the organism exhibited greater endo-xylanase than cell-associated β-xylosidase activity. Among inexpensive materials produced on saline lands, the salt tolerant grass Leptochloa fusca supported maximum xylanolytic activities followed by Sesbania aculeate (dhancha). The former could be effectively used for bulk production of xylanolytic enzymes by this organism.

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  1. National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan

    M. Ibrahim. Rajoka

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Correspondence to M. Ibrahim. Rajoka.

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Rajoka, M.I. Regulation of synthesis of endo-xylanase and β-xylosidase in Cellulomonas flavigena: a kinetic study. World J Microbiol Biotechnol 21, 463–469 (2005). https://doi.org/10.1007/s11274-004-2396-9

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  • DOI: https://doi.org/10.1007/s11274-004-2396-9

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