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Optimization of carotenoid production by Rhodotorula glutinis using statistical experimental design

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Abstract

A two-step optimization strategy of statistical experimental design was employed to enhance carotenoid production from sugar cane molasses (SCM) in the yeast Rhodotorula glutinis. In the first step, a fractional factorial design was used to evaluate the impact of five fermentation factors (pH and concentrations of SCM, urea, KH2PO4, and NaCl). The results revealed that three factors (concentrations of SCM, urea, and KH2PO4) had a significant influence on biomass and carotenoid production. A face-centered central composite design was then used in the second step to optimize the three significant factors to further enhance the biomass yield and carotenoid production. Through this two-step optimization strategy, the carotenoid concentration could be increased from an average of 1.39 mg/l to an average of 3.46 mg/l, representing a 2.5-fold carotenoid production enhancement.

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References

  • P. Bhosale R.V. Gadre (2001) ArticleTitleβ-Carotene production in sugarcane molasses by a Rhodotorula glutinis mutant Journal of Industrial Microbiology and Biotechnology 26 327–332 Occurrence Handle10.1038/sj.jim.7000138 Occurrence Handle11571614

    Article  PubMed  Google Scholar 

  • P. Buzzini (2000) ArticleTitleAn optimization study of carotenoid production by Rhodotorula glutinis DBVPG 3853 from substrates containing concentrated rectified grape must as the sole carbohydrate source Journal of Industrial Microbiology and Biotechnology 24 41–45 Occurrence Handle10.1038/sj.jim.2900765

    Article  Google Scholar 

  • P. Buzzini (2001) ArticleTitleBatch and fed-batch carotenoid production by Rhodotorula glutinis Debaryomyces castellii co-cultures in corn syrup Journal of Applied Microbiology 90 843–847 Occurrence Handle10.1046/j.1365-2672.2001.01319.x Occurrence Handle11348447

    Article  PubMed  Google Scholar 

  • J.A. Florencio C.R. Soccol L.F. Furlanetto T.M.B. Bonfim N. Krieger M. Baron J.D. Fontana (1998) ArticleTitleA factorial approach for sugarcane juice-based low cost culture medium: increasing the astaxanthin production by the red yeast Phaffia rhodozyma Bioprocess Engineering 19 161–164 Occurrence Handle10.1007/s004490050499

    Article  Google Scholar 

  • G. Frengova K. Simova K. Pavlova D. Beshkova D. Grigorova (1994) ArticleTitleFormation of carotenoids by Rhodotorula glutinis in whey ultrafiltrate Biotechnology and Bioengineering 44 888–894 Occurrence Handle10.1002/bit.260440804

    Article  Google Scholar 

  • Y. Goksungur Y. Mantzouridou T. Roukas (2002) ArticleTitleOptimization of the production of β-carotene from molasses by Blakeslea trispora: a statistical approach Journal of Chemical Technology and Biotechnology 77 933–943 Occurrence Handle10.1002/jctb.662

    Article  Google Scholar 

  • P.D. Haaland (1989) Experimental Design in Biotechnology Dekker New York

    Google Scholar 

  • M. Kennedy D. Krouse (1999) ArticleTitleStrategies for improving fermentation medium performance: a review Journal of Industrial Microbiology and Biotechnology 23 456–475 Occurrence Handle10.1038/sj.jim.2900755

    Article  Google Scholar 

  • E.Y. Kim P.K. Park H.J. Chae (1998) ArticleTitleOptimization of culture conditions for extracellular lipid production from Rhodotorula glutinis K-501 Korean Journal of Biotechnology and Bioengineering 13 58–64

    Google Scholar 

  • P.C. Lee C. Schmidt-Dannert (2002) ArticleTitleMetabolic engineering towards biotechnological production of carotenoids in microorganisms Applied Microbiology and Biotechnology 60 1–11 Occurrence Handle10.1007/s00253-002-1101-x Occurrence Handle12382037

    Article  PubMed  Google Scholar 

  • G.L. Miller (1959) ArticleTitleUse of dinitrosalicylic acid reagent for determination of reducing sugar Analytical Chemistry 31 426–429 Occurrence Handle10.1021/ac60147a030

    Article  Google Scholar 

  • R.H. Myers D.C. Montgomery (2002) Response Surface Methodology: Process and Product Optimization Using Designed Experiments John Wiley & Sons New York

    Google Scholar 

  • P.K. Park E.Y. Kim (2002) ArticleTitleExtraction method of carotenoids from Rhodotorula glutinis Korean Journal of Biotechnology and Bioengineering 17 44–48

    Google Scholar 

  • S. Siva Kesava G.-H. An C.-H. Kim S.-K. Rhee E.-S. Choi (1998) ArticleTitleAn industrial medium for improved production of carotenoids from a mutant strain of Phaffia rhodozyma Bioprocess Engineering 19 165–170 Occurrence Handle10.1007/s004490050500

    Article  Google Scholar 

  • D. Somashekar R. Joseph (2000) ArticleTitleInverse relationship between carotenoid and lipid formation in Rhodotorula gracilis according to the C/N ratio of the growth medium World Journal of Microbiology and Biotechnology 16 491–493 Occurrence Handle10.1023/A:1008917612616

    Article  Google Scholar 

  • M. Vazquez A.M. Martin (1998) ArticleTitleOptimization of Phaffia rhodozyma continuous culture through response surface methodology Biotechnology and Bioengineering 57 314–320 Occurrence Handle10.1002/(SICI)1097-0290(19980205)57:3<314::AID-BIT8>3.0.CO;2-K Occurrence Handle10099208

    Article  PubMed  Google Scholar 

  • D. Weuster-Botz (2000) ArticleTitleExperimental design for fermentation media development: statistical design or global random search? Journal of Bioscience and Bioengineering 90 473–473

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, The University of Seoul, Seoul, 130-743, Korea

    P. K. Park, D. H. Cho & E. Y. Kim

  2. Department of Chemical and Process Engineering, University of Canterbury, Private Bag 4800, Christchurch, New Zealand

    K. H. Chu

Authors
  1. P. K. Park
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  2. D. H. Cho
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  3. E. Y. Kim
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  4. K. H. Chu
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Correspondence to E. Y. Kim.

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Park, P.K., Cho, D.H., Kim, E.Y. et al. Optimization of carotenoid production by Rhodotorula glutinis using statistical experimental design. World J Microbiol Biotechnol 21, 429–434 (2005). https://doi.org/10.1007/s11274-004-1891-3

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  • DOI: https://doi.org/10.1007/s11274-004-1891-3

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