Abstract
Production of β-fructofuranosidase from the thermophilic fungus Thermoascus aurantiacuswas enhanced by optimization of the type of nitrogen source as well as the type and concentration of carbon source. Submerged batch cultivation in a laboratory bioreactor (7 l) using the optimized medium allowed the production of 85 mU/ml of β-fructofuranosidase. The enzyme showed both transfructosylating and hydrolytic activities and was optimally active at 60 °C and pH 5.0. The enzyme showed the ability to catalyse the synthesis of 1-kestose and the reaction was maximized at 30% (w/v) initial sucrose concentration.
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Katapodis, P., Christakopoulos, P. Induction and partial characterization of intracellular β from Thermoascus aurantiacus and its application in the synthesis of 6-kestose. World Journal of Microbiology and Biotechnology 20, 667–672 (2004). https://doi.org/10.1007/s11274-004-0684-z
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DOI: https://doi.org/10.1007/s11274-004-0684-z