Abstract
The growing production of beef has resulted in the increased volume of liquid residues generated during slaughtering and processing, known as slaughterhouse wastewater. In an attempt to reduce the organic content of this wastewater, blood has been separated from the water, generating slaughterhouse wastewater from green line slaughter and processing (SHWW in natura). The objective of this study was to determine the physical, chemical, and toxicological characteristics of SHWW in natura collected from a bovine slaughterhouse and locker. The results obtained from this characterization showed a high concentration of solids, total volatile acids, alkalinity, macro and micro nutrients, and organic matter (BOD5 20°C, COD, and DOC). The effluent presented acute toxicity to the test-organisms P. putida, E. coli, and D. similis, and chronic toxicity to the test-organisms C. silvestri, C. dúbia, E. coli, P putida, and P. subcaptata. Based on the ecotoxicological tests, it was concluded that the compounds of the SHWW in natura were more toxic to microcrustaceans and algae than to bacteria.
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Abreu, M. J., Santos-Wisniewski, M. J., Rocha, O., & Orlando, T. C. (2010). The use of PCR-RFLP to genetically distinguish the morphologically close species: Ceriodaphniadubia (Richard, 1894) and Ceriodaphniasilvestrii (Daday, 1902) (CrustaceaCladocera). Brazilian Journal Biology, 70(1), 121–124.
Aguilar, C. P., Peruzzolo, M., Di Luccio, M., Dallago, R. M., & Nascimento Filho, I. (2006). Qualitative study of organic compounds in wastewaters of a swine slaughterhouse. Environmental Monitoring and Assessment, 116, 103–110.
APHA—American Public Health Association; AWWA—American Water Works Association; WEF—Water Environment Federation. Standard methods for the examination of wand wastewater. 21th. ed. Washington. D.C.: APHA/AWWA/WEF, 2005
Barros Neto, B., Scarmínio, I. S., & Bruns, R. E. (2010). Como fazer experimentos. Editora Bookman: Porto Alegre. 413 p.
Brazilian Association of Technical Standards (BATS). NBR 12648: Aquatic ecotoxicology—chronic toxicity—test method with algae (Chlorophyceae). Rio de Janeiro. 2005.
Brazilian Association of Technical Standards (BATS). NBR 12713: Aquatic ecotoxicology—acute toxicity—test method Daphnia sp (Cladocera, Crustacea). Rio de Janeiro. 2009.
Brazilian Association of Technical Standards (BATS). NBR 13373: Aquatic ecotoxicology—chronic toxicity—test method Ceriodaphnia sp (Cladocera, Crustacea). Rio de Janeiro. 2010.
Cassidy, D. P., & Belia, E. (2005). Nitrogen and phosphorus removal from an abattoir wastewater in a SBR with aerobic granular sludge. Water Research, 39, 4817–4823.
Chaves, P. C., Castillo, L. R., Dendooven, L., & Escamilla-Silva, E. M. (2005). Poultry slaughterhouse wastewater treatment with an up-flow anaerobic sludge blanket (UASB) reactor. Bioresource Technology, 96(3), 1730–1736.
CONAMA—National Council of Environment. Resolution no 430. Ministry of Environment, Brasília, 2011. 8p.
CONAMA—National Council of Environment. Resolution no 357. Ministry of Environment, Brasília, 2005. 58p.
Dilallo, R., & Albertson, O. E. (1961). Volatile acids by direct titration. Journal Water Pollution Control Federation, 33(n. 4), 356–365.
Ferreira, D. F. (2011). Sisvar: a computer statistical analysis system. Ciencia e Agrotecnologia, 35(6), 1039–1042.
Gulley, D. D., Boelter, A. M., & Bergman, H. L. (1991). TOXTAT 3.3.: fish physiology and toxicology laboratory. Laramie: University of Wyoming.
Hamilton, M. A., Russo, R. C., & Thurfton, R. B. (1977). Trimmed Spearman–Karber method for estimating median lethal concentration in toxicity bioassays. Environmental Science and Technology, 11(n.4), 714–719.
Instituto Brasileiro De Geografia E Estatística (IBGE). Pesquisas Trimestrais do Abate de Animais, do Leite, do Couro e da Produção de Ovos de Galinha. Estatística da Produção Agropecuária 2013–2014. Acesso em:http://www.ibge.gov.br/home. 14 de março de 2015.
Jenkins, S. R., Morgan, J. M., & Sawyer, C. L. (1983). Measuring anaerobic sludge digestion and growth by a simple alkalimetric titration. Journal Water Pollution Control Federation, 55(5), 448–453.
Jia, Y., Gao, C., Zhang, L., & Jiang, G. (2012). Effects of pre-fermentation and influent temperature on the removal efficiency of COD, NH4 -N and PO3 -P in slaughterhouse wastewater by using SBR. Energy Procedia, 16, 1964–1971.
Kist, L. T., EL Moutaqi, S., & Machado, E. L. (2009). Cleaner production in the management of water use at a poultry slaughterhouse of Vale do Taquari, Brazil: a case study. Journal of Cleaner Production, 17(5), 1200–1205.
Lan, C. H., Lin, T. S., & Peng, C. Y. (2005). Aquatic toxicity of nitrogen mustard to Ceriodaphinadubia, Daphnia magna, and Pimephalespromelas. Ecotoxicology and Environmental Safety, 61, 273–279.
Lee, J., Park, J. H., Shin, Y. S., Lee, B. C., Chang, N., Cho, J., & Kim, S. D. (2009). Effect of dissolved organic matter on the growth of algae Pseudokirchneriellasubcapitata, in Korean lakes: the importance of complexation reactions. Ecotoxicology and Environmental Safety, 72, 335–343.
Li, J., Healy, M., Zhan, X., & Rodgers, M. (2008). Nutrient removal from slaughterhouse wastewater in an intermittently aerated sequencing batch reactor. Bioresource Technology, 99(2), 7644–7650.
Massé, D. I., & Massé, L. (2000). Characterization of wastewater from hog slaughterhouse in Eastern Canada and evaluation of their in plant wastewater treatment systems. Canadian Agricultural Engineering, 42(6), 139–146.
Merzouki, M., Bernet, N., Delgenes, J. P., & Benlemlih, M. (2005). Effect of pre-fermentation on denitrifying phosphorus removal in slaughterhouse wastewater. Bioresource Technology, 96, 1317–1322.
Metcalf, & Eddy. (2003). Wastewater engineering: treatment, disposal and reuse. New York: McGraw-Hill. 1334 p.
Nery, V. D., Pozzi, E., Damianovic, M. H. R. Z., Domingues, M. R., & Zaiat, M. (2008). Granules characteristics in the vertical profile of a full-scale upflow anaerobic sludge blanket reactor treating poultry slaughterhouse wastewater. Bioresourse Technology, 99(3), 2018–2024.
Norbert-King, T. J. A linear interpolation method for sublethal toxicity: the inhibition concentration (ICp) approach (Version 2.0). Dulunth: USEPA, 1993. Tech. Rept. 03–93. 25 p.
Pacheco, J. W., & Yamanaka, H. T. (2008). Environmental technician slaughter guide (beef and pork). São Paulo: The Environmental Sanitation Technology Company (ESTC). 98p.
Pereira, E. L. (2014) Treatment of slaughterhouse wastewater using a system consisting of reactors with biofilm. 214 p. Dissertation (Master of Science) – Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena/SP, 2014.
Pereira, E. L., Campos, C. M. M., & Motteran, F. (2013). Physicochemical study of pH, alkalinity and total acidity in a system composed of anaerobic baffled reactor in series with upflow anaerobic sludge blanket reactor in the treatment of pig farming wastewater. Acta Scientiarum Technology, 35, 477–483.
Ripley, L. E., Boyle, W. C., & Converse, J. C. (1986). Improved alkalimetric monitoring for anaerobic digestion of high-strength wastes. Journal Water Pollution Control Federation, 58(5), 406–411.
Rodrigues, L. B., Santos, L. R., Tagliari, V. Z., Rizzo, N. N., Trenhago, G., Oliveira, A. P., Goetz, F., Nascimento, V. P., & Rodrigues, L. B. (2010). Quantification of biofilm production on polystyrene by Listeria, Escherichia coliand Staphylococcus aureusisolated from a poultry slaughterhouse. Brazilian Journal of Microbiology, 41, 1082–1085.
Sarma, S. S. S., Ramírez, E. M., & Nandini, S. (2003). Effect of ammonia toxicity on the competition among three species of cladocerans (Crustacea:Cladocera). Ecotoxicology and Environmental Safety, 55, 227–235.
Sittijunda, S., Reungsang, A., & O-Thong, S. (2010). Biohydrogen production from dual digestion pretreatment of poultry slaughterhouse sludge by anaerobic self-fermentation. International Journal of Hydrogen Energy, 35(3), 13427–13434.
Slabbert, J. L. (1986). Improved bacterial growth test for rapid water toxicity screening. Bulletin of Environmental Contamination Toxicology, 37(3), 565–569.
United States Environmental Protection Agency (USEPA). Methods for measuring the acute toxicity of effluents to freshwater and marine organisms. EPA/600/4-85/013. 1985.
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The authors would like to thank the National Scientific and Technological Development Council (CNPq) for funding the research project. Thanks are also extended to Lucia A. B. A. Castro and Cleber M. Tomazi for their technical support.
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Pereira, E.L., de Paiva, T.C.B. & da Silva, F.T. Physico-chemical and Ecotoxicological Characterization of Slaughterhouse Wastewater Resulting from Green Line Slaughter. Water Air Soil Pollut 227, 199 (2016). https://doi.org/10.1007/s11270-016-2873-4
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DOI: https://doi.org/10.1007/s11270-016-2873-4