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Pyrene Metabolism by New Species Isolated from Soil Rhizoctonia Zeae SOL3

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Abstract

Rhizoctonia zeae SOL3 fungus was isolated from contaminated soil based on its ability to decolorize remazol brilliant blue R in solid medium. This fungus has been used to degrade pyrene a four-ring polycyclic aromatic hydrocarbon. R. zeae SOL3 could biodegrade pyrene as a sole source of carbon and energy. Different parameters were investigated to study their effect on the biodegradation rate. The highest biodegradation rate reached at 28 °C, non-agitated culture, 20 g/L glucose, 24 g/L NaCl, and 20 mg/L pyrene. The metabolites of pyrene were detected by thin layer chromatography (TLC) and confirmed by gas chromatography–mass spectrometry (GC-MS), which were identified as benzoic acid, 4-hydroxybenzoic acid and botanic acid.

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Acknowledgments

This work was supported by Fundamental Research Grant Scheme (FRGS) of Ministry of High Education (MOHE). (Vote No. R.J130000.7809.4F465 and UTM Institutional Research Grant. (Vote No. Q.J130000.2509.06H93)

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Authors and Affiliations

  1. Centre for Sustainable Environment and Water Security (IPASA), Research Institute for Sustainable Environment, Department of Environmental Engineering, Faculty of Civil Engineering, Universiti Teknologi Malaysia, UTM, 81310, Skudai, Johor, Malaysia

    Ameer Badr Khudhair, Tony Hadibarata, Abdull Rahim Mohd Yusoff, Zee Chuang Teh & Hesam Kamyab

  2. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, UTM, 81310, Johor Bahru, Johor, Malaysia

    Liyana Amalina Adnan

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  1. Ameer Badr Khudhair
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  2. Tony Hadibarata
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Correspondence to Ameer Badr Khudhair.

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Khudhair, A.B., Hadibarata, T., Yusoff, A.R.M. et al. Pyrene Metabolism by New Species Isolated from Soil Rhizoctonia Zeae SOL3. Water Air Soil Pollut 226, 186 (2015). https://doi.org/10.1007/s11270-015-2432-4

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