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Investigation of Organic Solvent Resistance Mechanisms in Vibrio alginolyticus IBBCt2

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Abstract

Constanta harbor has been contaminated for decades with petroleum and petroleum products, which contain different toxic organic solvents. A novel solvent-tolerant bacterium, Vibrio alginolyticus IBBCt2 was isolated from a seawater sample (Constanta harbor). Alkanes (i.e., n-hexane, n-decane, cyclohexane) with logarithm of partition coefficient in n-octanol and water (log P OW ) > 3.35, were less toxic for V. alginolyticus strain IBBCt2, compared with aromatics (i.e., toluene, m-xylene, ethylbenzene) with log P OW  < 3.17. The high organic solvent resistance of V. alginolyticus IBBCt2 could be due to the presence of some catabolic (alkB, alkB/alkB1, todC1, xylM, C23DO) and transporter (HAE1, acrAB) genes. The adaptation mechanisms, underlying cyclohexane, n-hexane, n-decane, toluene, m-xylene, and ethylbenzene resistance in V. alginolyticus IBBCt2 showed a complex response of cells 60 min after solvent shock (i.e., modifications of the cell viability, changes in the membrane’s lipid and protein profile, modifications of the genomic fingerprinting). Exposure of V. alginolyticus IBBCt2 cells to salt stress decreases the organic solvents tolerance of this bacterium.

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Acknowledgments

The study was funded by project no. RO1567-IBB05/2012 from the Institute of Biology Bucharest of Romanian Academy. The author is grateful to Ana Dinu for technical support.

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  1. Institute of Biology Bucharest of Romanian Academy, 296 Splaiul Independentei, 060031, Bucharest , P.O. Box 56-53, Romania

    Mihaela Marilena Stancu

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Stancu, M.M. Investigation of Organic Solvent Resistance Mechanisms in Vibrio alginolyticus IBBCt2 . Water Air Soil Pollut 223, 4495–4505 (2012). https://doi.org/10.1007/s11270-012-1212-7

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