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Cupric Reductase Activity in Copper-Resistant Amycolatopsis tucumanensis

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Abstract

Amycolatopsis tucumanensis, a recently recognized novel species showed remarkable copper resistance as well as efficient Specific Cupric Reductase Activity (SRACu) in both, copper adapted and non-adapted cells, under different temperatures of incubation. Its copper resistance strength was highlighted against other metal-resistant actinobacteria (Streptomyces sp. AB5A) and sensitive strains (Amycolatopsis eurytherma and Streptomyces coelicolor). Pre-adapted cells of A. tucumanensis displayed values of SRACu, on average, 65% higher than those obtained from non-adapted cells. In addition, preadaptation of A. tucumanensis improved the rate of Cu(II) reduction which was approximately, two-, seven- and ninefold higher than pre-adapted cells from Streptomyces sp. AB5A, A. eurytherma and S. coelicolor, respectively. A. tucumanensis showed the highest levels of SRACu at all temperatures and also the highest copper resistance profile, suggesting that these two abilities may be in close relationship. This ostensible versatility, related to the temperature, of adapted cells from A. tucumanensis might support the application of this strain under different bioremediation conditions. To our knowledge this is the first time that cupric reductase activity was demonstrated within the genus Amycolatopsis.

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Acknowledgements

This work was supported by CIUNT, FONCyT, CONICET, Argentina. J.S.D.C. is supported by a FONCyT doctoral scholarship while V.H.A. and C.M.A. are researchers from the National Research Council in Argentina. The authors gratefully acknowledge the assistance of Mr. Guillermo Borchia.

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Authors and Affiliations

  1. Pilot Plant of Industrial and Microbiological Processes (PROIMI), CONICET, Av. Belgrano y Pasaje Caseros, 4000, Tucumán, Argentina

    José Sebastián Dávila Costa, Virginia Helena Albarracín & Carlos Mauricio Abate

  2. Natural Sciences College and Miguel Lillo Institute, National University of Tucumán, 4000, Tucumán, Argentina

    Virginia Helena Albarracín & Carlos Mauricio Abate

  3. Biochemistry, Chemistry and Pharmacy College, National University of Tucumán, 4000, Tucumán, Argentina

    Carlos Mauricio Abate

  4. PROIMI-CCT, Av. Belgrano y Pasaje Caseros, 4000, Tucumán, Argentina

    Carlos Mauricio Abate

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  1. José Sebastián Dávila Costa
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  2. Virginia Helena Albarracín
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  3. Carlos Mauricio Abate
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Correspondence to Carlos Mauricio Abate.

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Dávila Costa, J.S., Albarracín, V.H. & Abate, C.M. Cupric Reductase Activity in Copper-Resistant Amycolatopsis tucumanensis . Water Air Soil Pollut 216, 527–535 (2011). https://doi.org/10.1007/s11270-010-0550-6

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  • DOI: https://doi.org/10.1007/s11270-010-0550-6

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