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3-Chlorophenol Biodegradation in a Sequencing Batch Reactor: Kinetic Study and Effect of the Filling Time

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Abstract

Biodegradation kinetics of 3-chlorophenol (3-CP) were studied in two identical lab scale sequencing batch reactors (SBR) fed with the compound as the sole energy and carbon source and operated at different filling time (1 h for SBR1 and 2 h for SBR2). High removal efficiency was always obtained in both SBRs in the range of feed concentration of 300–960 mg L−1. Increased feed load to 1,200 mg L−1 3-CP could also be removed in SBR1 despite the presence of inhibition, whereas determined failure of SBR2. Long filling time and high biomass concentration were shown to have beneficial effect on process kinetics since they allowed to avoid substrate concentration peaks at the end of the fill phase. However, longer filling time (in the present case higher than 1 h) did not allow to select and enrich robust microbial population. The Haldane equation well fitted the kinetic test data measured in the presence of inhibition, i.e., at 960 and 1,200 mg L−1 3-CP in SBR1.

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Abbreviations

SBR:

Sequencing batch reactor

CFSTR:

Continuous flow stirred tank reactor

DO:

Dissolved oxygen concentration (mg O2 L−1)

OUR:

Oxygen uptake rate (mg O2/L−1 h−1)

V TOT :

Working volume (L)

t FILL :

Filling time (h)

V IN :

Influent volume (L)

3-CP:

3-chlorophenol

C IN :

3-CP influent concentration (mg L−1)

COD:

Chemical oxygen demand (mg L−1)

MLSS:

Mixed liquor suspended solids (mg L−1)

MLVSS:

Mixed liquor volatile suspended solids (mg L−1)

TSS:

Total suspended solids (mg L−1)

VER:

Volumetric exchange rate (adim.)

VSS:

Volatile suspended solids (mg L−1)

Y :

Growth yield coefficient (mg VSS mg COD−1)

b :

Endogenous respiration rate (day−1)

SVI:

Sludge volume index (mL g−1)

F co :

Influent organic loading factor (mg COD mg VSS−1 day−1)

F cv :

Volumetric organic loading factor (g COD L−1 day−1)

k max :

Maximum specific removal rate (mg COD mg VSS−1 day−1)

k*:

Real maximum specific removal rate (mg COD mg VSS−1 day−1)

U(S):

Substrate specific utilization rate (mg COD mg VSS−1 h−1)

K s :

Saturation constant (mg L−1)

K I :

Inhibition constant (mg L−1)

μ :

Maximum specific growth rate (day−1)

μ*:

Real maximum specific growth rate (day−1)

C*:

Critical substrate concentration (mg L−1)

\( C_{{3} - {\rm{CP,}}i}^{{ \exp }} \) :

Experimental 3-CP concentration (mg L−1)

\( C_{{3} - {\rm{CP,}}i}^{\rm{cal}} \) :

Modeled 3-CP concentration (mg L−1)

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Authors and Affiliations

  1. Department of Hydraulics, Transportation and Roads, University of Rome Sapienza, Via Eudossiana 18, 00184, Rome, Italy

    Agostina Chiavola

  2. Department of Civil Engineering, University of Rome Tor Vergata, Via del Politecnico, 1, 00133, Rome, Italy

    Renato Baciocchi

  3. Acea Ato 2S.p.A., Piazzale Ostiense 2, 00154, Rome, Italy

    Federico Barducci

Authors
  1. Agostina Chiavola
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  2. Renato Baciocchi
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  3. Federico Barducci
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Correspondence to Agostina Chiavola.

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Chiavola, A., Baciocchi, R. & Barducci, F. 3-Chlorophenol Biodegradation in a Sequencing Batch Reactor: Kinetic Study and Effect of the Filling Time. Water Air Soil Pollut 212, 219–229 (2010). https://doi.org/10.1007/s11270-010-0334-z

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  • DOI: https://doi.org/10.1007/s11270-010-0334-z

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