Skip to main content
SpringerLink
Log in

Bioremediation of Lindane Contaminated Soil by Pleurotus ostreatus in Non Sterile Conditions Using Multilevel Factorial Design

  • Published:
Water, Air, and Soil Pollution Aims and scope Submit manuscript

Abstract

A commercial strain of the fungus Pleurotus ostreatus was used to mediate the degradation of lindane, by landfarming technology during a 4 weeks statistical experiment. The Multilevel Factorial Design was used with two design factors, namely, straw content X 1 (%) and lindane content X 2 (ppm). The optimization parameters (responses) investigated were: biodegradation rate Y 1 (μg d−1), biomass growth rate Y 2 (mg d−1), biodegradation/biomass Y 3 (μg mg−1), total organic carbon Y 4 (%), total organic nitrogen Y 5 (%) and total organic carbon/total organic nitrogen Y 6. The optima of the adequate models obtained for the period of 2 and 4-weeks were found. An overall kinetic study, conducted in this work with the aid of experimental design, determined the optimum (maximum) specific lindane degradation rate to be 0.16 g kg−1 month−1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Aidoo, K. E., Hendry, R., & Wood, B. J. B. (1981). Estimation of fungal growth in a solid state fermentation system. European Journal of Applied Microbiology, 12, 6–9.

    Article  CAS  Google Scholar 

  • Boyle, C. D. (1995). Development of practical method for inducing white rot fungi to grow into and degrade organopollutants in soil. Canadian Journal of Microbiology, 41, 345–353.

    Article  CAS  Google Scholar 

  • Gavrilescu, M. (2005). Fate of pesticides in the environment and its bioremediation. Engineering in Life Science, 5, 497–525.

    Article  CAS  Google Scholar 

  • Kirk, T. K., Lamar, T., & Glaser, J. A. (1992). The potential of white rot fungi in bioremediation.. In S. Mongkolsuk, P. S. Lovett, & J. E. Trempy (Eds.), Biotechnology and environmental science: Molecular approaches (pp. 131–138). New York: Plenum.

    Chapter  Google Scholar 

  • Kumar, M., Chaudhary, P., Dwivedi, M., Kumar, R., Debarati, P., Jain, R., et al. (2005). Enhanced biodegradation of b- and δ-hexachlorocyclohexane in the presence of a- and δ-isomers in contaminated soils. Environmental Science & Technology, 39, 4005–4011.

    Article  CAS  Google Scholar 

  • Papadopoulou, K., Rigas, F., & Doulia, D. (2006a). Bioremediation of Lindane contaminated soil by the white rot fungus Pleurotus ostreatus via fractional Central Composite Design. In IASME/WSEAS International Conference on Energy, Environment, Ecosystems & Sustainable Development, Greece.

  • Papadopoulou, K., Rigas, F., & Doulia, D. (2006b). Lindane degradation in soil by Pleurotus ostreatus. WSEAS Transactions on Environment and Development, 5(2), 489–496.

    Google Scholar 

  • Philippoussis, A., Zervakis, G., & Diamantopoulou, P. (2001). Bioconversion of lignocellulosic wastes through the cultivation on the edible mushrooms Agrocybe aegerita, Volvariella volvacea, and Pleurotus spp. World Journal of Microbiology & Biotechnology, 17, 191–200.

    Article  CAS  Google Scholar 

  • Phillips, T. M., Lee, H., Trevors, J. T., & Seech, A. G. (2006). Full-scale in situ bioremediation of hexachlorocyclohexane-contaminated soil. Journal of Chemical Technology and Biotechnology, 81, 289–298.

    Article  CAS  Google Scholar 

  • Pointing, S. (2001). Feasibility of bioremediation by white rot fungi. Applied Microbiology and Biotechnology, 57, 20–33.

    Article  CAS  Google Scholar 

  • Ride, J. P., & Drysdale, R. B. (1972). A rapid method for the chemical estimation of filamentous fungi in plant tissue. Physiological Plant Pathology, 2, 7–15.

    Article  CAS  Google Scholar 

  • Rigas, F., Dritsa, V., Marchant, R., Papadopoulou, K., Avramides, E. J., & Hatzianestis, I. (2003). Biodegradation of lindane by Pleurotus ostreatus via central composite design. In 2nd European Bioremediation Conference, Chania, Greece.

  • Rigas, F., Dritsa, V., Marchant, R., Papadopoulou, K., Avramides, E. J., & Hatzianestis, I. (2005a). Biodegradation of lindane by Pleurotus ostreatus via central composite design. Environment International, 31, 191–196.

    Article  CAS  Google Scholar 

  • Rigas, F., Papadopoulou, K., Dritsa, V., & Doulia, D. (2007). Bioremediation of a soil contaminated by lindane utilizing the fungus Ganoderma australe via response surface methodology. Journal of Hazardous Materials, 140, 325–332.

    Article  CAS  Google Scholar 

  • Rigas, F., Papadopoulou, K., Dritsa, V., & Marchant, R. (2005b). Bioremediation of soil polluted with Lindane by Pleurotus ostreatus. In 3rd European Bioremediation Conference, Chania, Greece.

  • Scotti, T. C., Vergoignan, C., Feron, G., & Durand, A. (2001). Glucosamine measurement as indirect method for biomass estimation of Cunninghamella elegans grown in solid state cultivation conditions. Biochemical Engineering Journal, 7, 1–5.

    Article  CAS  Google Scholar 

  • Shah, M. M., Barr, D. P., Chung, N., & Aust, A. D. (1992). Use of white rot fungi in degradation of environmental chemicals. Toxicology Letters, 64, 493–501.

    Article  Google Scholar 

  • Viladi, M. (2001). Bioremediation. An overview. Pure and Applied Chemistry, 73, 1163–1172.

    Article  Google Scholar 

Download references

Acknowledgement

This work was supported by the grant 01ED45 from the Greek General Secretariat of Research and Technology under the PENED 2001 programme.

Author information

Authors and Affiliations

  1. School of Chemical Engineering, National Technical University of Athens, Iroon Polytechniou 9, Zografou, 15700, Athens, Greece

    F. Rigas, K. Papadopoulou & M. Papadopoulou

  2. Laboratory of Edible Fungi, National Agricultural Research Foundation, 61 Democratias St., 135 61 Ag. Anargyri, Athens, Greece

    A. Philippoussis

  3. General and Agricultural Microbiology Laboratory, Agricultural University of Athens, Athens, Greece

    J. Chatzipavlidis

Authors
  1. F. Rigas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Papadopoulou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Philippoussis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Papadopoulou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Chatzipavlidis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to F. Rigas.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rigas, F., Papadopoulou, K., Philippoussis, A. et al. Bioremediation of Lindane Contaminated Soil by Pleurotus ostreatus in Non Sterile Conditions Using Multilevel Factorial Design. Water Air Soil Pollut 197, 121–129 (2009). https://doi.org/10.1007/s11270-008-9795-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11270-008-9795-8

Keywords

Search

Navigation