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Changes in Extractability of Cr and Pb in a Polycontaminated Soil After Bioaugmentation With Microbial Producers of Biosurfactants, Organic Acids and Siderophores

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Water, Air, & Soil Pollution: Focus

Abstract

Partly because of the low bioavailability of metals, the soil cleaning-up using phytoremediation is usually time-consuming. In order to enhance the amount of metals at the plant's disposal, the soil bioaugmentation coupled together with phytoextraction is an emerging technology. In this preliminary work, two agricultural soils which mainly differed in their Cr, Hg and Pb contents (LC, low-contaminated soil; HC, high-contaminated soil) were bioaugmented in laboratory conditions by either bacterial (Bacillus subtilis, Pseudomonas aeruginosa, Pseudomonas fluorescens or Ralstonia metallidurans) or fungal inocula (Aspergillus niger or Penicillium simplicissimum) and incubated during three weeks. The LC soil pots bioaugmented with A. niger and P. aeruginosa contained higher concentrations of Cr (0.08 and 0.25 mg.kg−1 dw soil) and Pb (0.25 and 0.3 mg.kg−1 dw soil) in the exchangeable fraction F1 (extraction with MgCl2) by comparison with the non-bioaugmented soil where neither Cr nor Pb was detected. Conversely, immobilization of Cr and Pb in the soil were observed with the other microorganisms. The soil bioaugmentation not only modified the metal speciation for the most easily extractable fractions but also modified the distribution of metals in the other fractions, to a lesser extent nevertheless. The difference in microbial concentrations between the bioaugmented or not HC soils reached up to 1.8 log units. Thus the microorganisms that we chose for the soil bioaugmentation were competitive towards the indigenous microflora. The PCA analysis showed close positive relationships between the microorganisms which potentially produced siderophores in the soil and the amount of Cr and Pb in the fraction F1.

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Authors and Affiliations

  1. Laboratoire Gestion des Risques et Environnement (EA 2334), Université de Haute-Alsace, Antenne de Colmar, BP 50568, 68 008, Colmar cedex, France

    Armelle Braud, Karine Jézéquel, Emilie Vieille, Adrien Tritter & Thierry Lebeau

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  1. Armelle Braud
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  2. Karine Jézéquel
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  3. Emilie Vieille
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  5. Thierry Lebeau
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Correspondence to Thierry Lebeau.

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Braud, A., Jézéquel, K., Vieille, E. et al. Changes in Extractability of Cr and Pb in a Polycontaminated Soil After Bioaugmentation With Microbial Producers of Biosurfactants, Organic Acids and Siderophores. Water Air Soil Pollut: Focus 6, 261–279 (2006). https://doi.org/10.1007/s11267-005-9022-1

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