Abstract
The question of whether some cases of interstitial cystitis may have an infectious etiology has been debated for some time. Previous studies have looked for the presence of certain specific viruses, but generally did not use the types of sensitive and unbiased approaches that are currently available. As part of the MAPP (Multidisciplinary Approach to the Study of Chronic Pelvic Pain) Research Network, we examined urine specimens from interstitial cystitis patients who provided specimens over time and also reported various symptoms at the time of urine collection. We first performed next-generation sequencing to look for the presence of viruses in urines, and detected two human polyomaviruses that are known to be excreted into urine, BKPyV and JCPyV. We were especially interested in BKPyV because it is a known cause of another bladder disease, hemorrhagic cystitis, in bone marrow transplant recipients. Further analysis of individual samples indicates a trend toward higher excretion of polyomaviruses in patients experiencing increased symptoms.
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Acknowledgements
This work was performed as part of an ancillary award to MJI and JMP from the Multidisciplinary Approach to the Study of Chronic Pelvic Pain research network (MAPP), funded by the NIDDK (DK82370, DK82342, DK82315, DK82344, DK82325, DK82345, DK82333, and DK82316).
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MS-R designed and performed experiments, contributed to data analysis, and contributed to writing the manuscript. PGC performed data analysis. AMD performed experiments. MM performed experiments. MF contributed to experimental design and performed experiments. AvP prepared and oversaw blinding of the specimens, and contributed to the writing. AS-S performed the statistical analyses and contributed to the writing of the manuscript. JMP designed the study, contributed to data analysis, and contributed to writing the manuscript. MJI designed the study, contributed to data analysis, and led preparation of the manuscript.
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All subjects in this study were enrolled at one of six MAPP participating centers, each of whose IRBs approved the protocol. The subjects were consented at the time of enrollment at each center.
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Robles, M.T.S., Cantalupo, P.G., Duray, A.M. et al. Analysis of viruses present in urine from patients with interstitial cystitis. Virus Genes 56, 430–438 (2020). https://doi.org/10.1007/s11262-020-01767-z
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DOI: https://doi.org/10.1007/s11262-020-01767-z