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Genetic characterization of bovine ephemeral fever virus in southern China, 2013–2017

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Abstract

Bovine ephemeral fever virus (BEFV) can cause bovine ephemeral fever and is an economically important arbovirus of cattle. To expand the knowledge of the molecular epidemiology of BEFV in southern China, the complete surface glycoprotein G gene of BEFV was sequenced from samples collected in five restricted outbreaks from 2013 to 2017, namely 2013ZH, 2014HM, 2015GX, 11082-2016, and qy2017. It was noted that both 2014HM and 11082-2016 were detected in cattle regularly vaccinated with inactivated vaccine. Phylogenetic analysis demonstrated that all five strains grouped into cluster I. However, qy2017 was closer to the BEFV strains identified in Thailand, Japan, and Taiwan after 2000, while 2013ZH, 2014HM, 2015GX, and 11082-2016 were closer to the Chinese strains in 2011 and the Turkey strains in 2012. The analysis of antigenic sites indicated that several amino acid changes occurred between the five strains and the vaccine strain. Importantly, one novel amino acid mutation site was observed in the putative N-linked glycosylation sites of 2013ZH, 2014HM, 2015GX, and 11082-2016. Our study indicated novel genetic characteristics of the newly emerging BEFV strains in southern China and the necessity of updating the component of commercially available inactivated BEFV vaccines in China.

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References

  1. S Nandi BS Negi 1999 Bovine ephemeral fever: a review Comp Immunol Microb 22 81 91

    Article  CAS  Google Scholar 

  2. WB Bai FL Tian C Wang CL Jiang ZG Zhang 1987 Preliminary studies of thecomplement fixation test to confirm the diagnosis of bovine ephemeral fever Aust J Biol Sci 40 137 141

    Article  CAS  Google Scholar 

  3. PJ Walker E Klement 2015 Epidemiology and control of bovine ephemeral fever Vet Res 46 124

    Article  Google Scholar 

  4. D Liu K Li L Zhang Y Lan X Wang H Zhang L Wang HZ GuiR W Jang S Sizhu J Li 2017 Seroprevalence investigation of bovine ephemeral fever in yaks in Tibetan Plateau of China from 2012 to 2015 Trop Anim Health Prod 2017 49 227 230

    Article  Google Scholar 

  5. L Trinidad KR Blasdell DA Joubert SS Davis L Melville PD Kirkland F Coulibaly EC Holmes PJ Walker 2014 Evolution of bovine ephemeral fever virus in the Australian episystem J Virol 88 3 1525 1535

    Article  Google Scholar 

  6. A Theodoridis EM Nevill HJ Els ST Boshoff 1979 Viruses isolated from Culicoides midges in South Africa during unsuccessful attempts to isolate bovine ephemeral fever virus Onderstepoort J Vet Res 46 4 191 198

    CAS  PubMed  Google Scholar 

  7. PJ Walker Y Wang JA Cowley SM McWilliam CJ Prehaud 1994 Structural and antigenic analysis of the nucleoprotein of bovine ephemeral fever rhabdovirus J Gen Virol 75 1889 1899

    Article  CAS  Google Scholar 

  8. PJ Walker KR Blasdell DA Joubert 2012 Ephemeroviruses: Arthropod-borne rhabdoviruses of ruminants, with large and complex genomes RG Dietzgen IM Kuzmin Eds Rhabdoviruses: molecular taxonomy, evolution, genomics, ecology, Host-vector interactions, cytopathology and control Caister Academic Press UK 59 88

    Google Scholar 

  9. J Francis 1976 An attenuated vaccine against bovine ephemeral fever Aust Vet J 52 11 537 538

    Article  CAS  Google Scholar 

  10. JD Thompson DG Higgins TJ Gibson 1994 CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrixchoice Nucleic Acids Res 22 4673 4680

    Article  CAS  Google Scholar 

  11. M Kimura 1980 A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences J Mol Evol 16 111 120

    Article  CAS  Google Scholar 

  12. J Felsenstein 1985 Confidence limits on phylogenies: an approach using thebootstrap Evolution 39 783 791

    Article  Google Scholar 

  13. K Tamura D Peterson N Peterson G Stecher M Nei S Kumar 2011 MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods MolBiol Evol 28 2731 2739

    Article  CAS  Google Scholar 

  14. N Saitou M Nei 1987 The neighbor-joining method: a new method for reconstructing phylogenetic trees MolBiolEvol 4 406 425

    CAS  Google Scholar 

  15. T Kato M Aizawa K Takayoshi T Kokuba T Yanase H Shirafuji T Tsuda M Yamakawa 2009 Phylogenetic relationships of the G gene sequence of bovine ephemeral fever virus isolated in Japan Taiwan and Australia Vet Microbiol 137 3–4 217 223

    Article  CAS  Google Scholar 

  16. FY Zheng CQ Qiu 2012 Phylogenetic relationships of the glycoprotein gene of bovine ephemeral fever virus isolated from mainland China, Taiwan, Japan, Turkey Israel and Australia Virol J 9 268

    Article  CAS  Google Scholar 

  17. T Chaisirirata P Sangthong P Arunvipas N Petcharat N Thangthamniyom W Chumsing P Lekcharoensuk 2018 Molecular characterization of bovine ephemeral fever virus in Thailand between 2013 and 2017 Vet Microbiol 227 1 7

    Article  Google Scholar 

Download references

Funding

This project was supported in part by the Guangdong Natural Science Foundation (2017A030310032), the Science and Technology Planning Project of Guangdong Province (2015B020203005), Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases (2017B030314142), Modern Agricultural Science and Technology Innovation Alliance of Guangdong Province (2018LM2150), Pearl River S and T Nova Program of Guangzhou (201610010073), The China Scholarship Council (201807630008).

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Author notes

  1. Jia Kun, Jia Rongrong and Wang Xiangbin have contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Jia Kun, Jia Rongrong, Wang Xiangbin, Zhao Yan, Dou Yiping, Lu Gang, Zhou Pei & Li Shoujun

  2. Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, 510642, People’s Republic of China

    Jia Kun, Jia Rongrong, Wang Xiangbin, Zhao Yan, Dou Yiping, Lu Gang, Zhou Pei & Li Shoujun

  3. Guangdong Technological Engineering Research Center for Pet, Guangzhou, 510642, People’s Republic of China

    Jia Kun, Jia Rongrong, Wang Xiangbin, Zhao Yan, Dou Yiping, Lu Gang, Zhou Pei & Li Shoujun

Authors
  1. Jia Kun
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  2. Jia Rongrong
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  3. Wang Xiangbin
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  4. Zhao Yan
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  5. Dou Yiping
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  6. Lu Gang
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  7. Zhou Pei
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  8. Li Shoujun
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Contributions

KJ carried out the studies and drafted the manuscript, XW, RJ, YZ, YD, PZ and GL carried out part of the studies, SL gave the polish to the language of the manuscript. All the authors read and approved the final paper.

Corresponding author

Correspondence to Li Shoujun.

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The authors declare that they have no competing interests.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Informed consent was obtained from all individual participants included in the study.

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Edited by Zhen F. Fu.

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Kun, J., Rongrong, J., Xiangbin, W. et al. Genetic characterization of bovine ephemeral fever virus in southern China, 2013–2017. Virus Genes 56, 390–395 (2020). https://doi.org/10.1007/s11262-020-01740-w

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  • DOI: https://doi.org/10.1007/s11262-020-01740-w

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