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Genetic diversity of bovine Picobirnavirus, Brazil

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Abstract

Picobirnaviruses (PBVs) are emerging and opportunistic viruses with possible zoonotic potential. In this study, we present the detection, molecular characterization, and genotypic differentiation of PBVs from genogroup I in bovine stool samples from different Brazilian regions. A high proportion of PCR-positive samples (23.4%) was detected in a total of 77 analyzed. Nucleotide identity, alignment, and phylogenetic analyses revealed high diversity among the studied sequences. The results obtained indicate, for the first time, the circulation of bovine PBVs belonging to genogroup I in different Brazilian states, with heterogeneous phylogenetic-clustering profiles.

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Acknowledgements

This research was funded by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Nos. 2006/52060-3 and 2012/18441-0) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, No. 472509/2010-1).

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Authors and Affiliations

  1. Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenue Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil

    Juliana de Oliveira Navarro, Marcelo Candido, Sabrina Ribeiro de Almeida-Queiroz, Andrezza Maria Fernandes & Ricardo Luiz Moro de Sousa

  2. Department of Preventive Veterinary Medicine and Animal Reproduction, São Paulo State University (UNESP), Access Route Prof. Paulo Donato Castellani, rural, Jaboticabal, São Paulo, 14884-900, Brazil

    Maria da Glória Buzinaro

  3. Department of Clinical Analysis, Alfenas Federal University (UNIFAL), Street Gabriel Monteiro da Silva, 700, Center, Alfenas, Minas Gerais, 37130-000, Brazil

    Márcia Cristina Livonesi

Authors
  1. Juliana de Oliveira Navarro
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  2. Marcelo Candido
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  3. Sabrina Ribeiro de Almeida-Queiroz
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  4. Maria da Glória Buzinaro
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  5. Márcia Cristina Livonesi
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  6. Andrezza Maria Fernandes
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  7. Ricardo Luiz Moro de Sousa
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Contributions

JON, MC, SRAQ, MGB, MCL, and RLMS conceived and designed the experiments. JON and MC performed the experiments. JON, MC, SRAQ, and RLMS analyzed the data. JON, MC, and RLMS wrote the manuscript. AMF participated in revising the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Marcelo Candido.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Edited by William Dundon.

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Navarro, J.O., Candido, M., de Almeida-Queiroz, S.R. et al. Genetic diversity of bovine Picobirnavirus, Brazil. Virus Genes 54, 724–728 (2018). https://doi.org/10.1007/s11262-018-1586-8

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  • DOI: https://doi.org/10.1007/s11262-018-1586-8

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