Abstract
The emergence and disappearance of antigenic variants of foot-and-mouth disease virus (FMDV) during a field outbreak occurs periodically due to the volatile nature of its genome. In the present analysis, change in antigenic behavior of serotype O FMDV during the serial cytolytic passage in the absence of immune pressure was observed. Initially, the isolate showed a poor antigenic match (relationship value <0.3) with the serotype O vaccine strain and upon serial passage increase in relationship value was observed. Comparison of capsid sequence revealed substitution at four positions (VP3:K58 → E and P158 → S, VP1:E83 → K and R172 → Q) acquired during the serial passage. Examination of passage level and amino acid substitution revealed the critical role of position VP3-58 that was identified earlier as crucial for antigenic site IV, in the observed antigenic variability. The role of position VP3-58 was further confirmed using reverse genetics approach.
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We sincerely thank all the scientific and technical staff associated with network laboratories for providing clinical samples and required data.
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SS conceived the study. BD carried out the work. JKB carried out reverse genetic study. RR helped in design of the experiment. SS, BD, and BP wrote the manuscript. All authors read and approved the final manuscript.
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Subramaniam, S., Das, B., Biswal, J.K. et al. Antigenic variability of foot-and-mouth disease virus serotype O during serial cytolytic passage. Virus Genes 53, 931–934 (2017). https://doi.org/10.1007/s11262-017-1494-3
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DOI: https://doi.org/10.1007/s11262-017-1494-3