Abstract
A bacterial artificial chromosome clone, designated LCY, was constructed from a Gallid herpesvirus 2 (GaHV-2) isolate from a GaHV-2 and reticuloendotheliosis virus co-infected clinical sample. The LCY GaHV-2 insert was sequenced and found to consist of 175,319 nucleotides. LCY GaHV-2 open reading frames (ORFs) had a high sequence identity to those of reference strains. The major difference was that two REV long terminal repeats (LTRs), in the same direction, were inserted at the internal repeat short (IRs)/unique short (Us) and Us/terminal repeat short (TRs) junctions. In addition, the a-like sequence and UL36 were different from other strains. Phylogenetic analysis revealed that LCY was closely related to pandemic strains in China. A pathogenicity study and a vaccination-challenge test were performed on LCY and the reference strain, GA. The results showed that LCY induced gross Marek’s disease (MD) lesions and mortality in 71.4 and 7.1% of chickens, respectively, which are lower rates than those observed for the reference strain GA (85.7 and 35.7%). The commercially available CVI988 vaccine provided complete protection against LCY and GA (100%). These results showed that the isolate exhibited lower pathogenicity in SPF chickens. This study revealed that a novel pattern of LTR inserts was found in the strain LCY and that the strain was of low virulence. The present work expands the available genetic information for GaHV-2 and will be useful for the control of MD in China.
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Acknowledgements
This study was funded by Grants from the National Natural Science Foundation of China (No. 31670155), the Application Technology Research and Development Fund of Harbin (No. 2016AB3BN034), and the National “Twelfth Five-Year” Plan for Science & Technology Support (2015BAD12B03).
Author contributions
C.J.L. and X.M.W. conceived and designed the study. Y.P.Z. and K.Y.B. carried out the experiments and wrote the manuscript. G.R.S. and H.C.L. analysed the data. H.Y.C. and Y.L.G. carried out the virulence studies. All authors read, revised, and approved the final manuscript.
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The animal experiments were approved by the Animal Ethics Committee of the Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences (CAAS) and were performed in accordance with animal ethics guidelines and approved protocols. The Animal Ethics Committee approval number was SYXK (hei) 2011-022.
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Yan-ping Zhang and Ke-yan Bao have contributed equally to this work.
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Zhang, Yp., Bao, Ky., Sun, Gr. et al. Characterization of a Gallid herpesvirus 2 strain with novel reticuloendotheliosis virus long terminal repeat inserts. Virus Genes 53, 386–391 (2017). https://doi.org/10.1007/s11262-017-1427-1
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DOI: https://doi.org/10.1007/s11262-017-1427-1