Abstract
Pepo aphid-borne yellows virus (PABYV) has been proposed as a putative representative of a new species in the genus Polerovirus in the family Luteoviridae. The genomes of two South African (SA) isolates of cucurbit-infecting PABYV were described in this record. Total RNA, extracted from a pattypan (Cucurbita pepo L.) and a baby marrow (C. pepo L.) leaf samples, was subjected to next-generation sequencing (NGS) on the HiSeq Illumina platform. Sanger sequencing was subsequently used to authenticate the integrity of PABYV’s genome generated from de novo assembly of the NGS data. PABYV genome of SA isolates consists of 5813 nucleotides and displays an organisation typical of poleroviruses. Genome sequence comparisons of the SA PABYV isolates to other poleroviruses support the classification of PABYV as a new species in the genus Polerovirus. Recombination analyses showed that PABYV and Cucurbit aphid-borne yellows virus (CABYV) shared the same ancestor for the genome part situated between breaking points. Phylogenetic analyses of the RNA-dependent RNA polymerase and the coat protein genes showed that SA PABYV isolates shared distant relationship with CABYV and Suakwa aphid-borne yellows virus. Based on our results, we propose that PABYV is a distinct species in the genus Polerovirus.
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Acknowledgments
The Authors thank Mr. Jonathan Featherston and Ms. Thulile Faith Nhlapo, from the ARC-BTP, for providing technical assistance with NGS. Sanger sequencing was performed at Inqaba Biotechnical Industries (Pty) Ltd (Pretoria, RSA).
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Ibaba, J.D., Laing, M.D. & Gubba, A. Pepo aphid-borne yellows virus: a new species in the genus Polerovirus . Virus Genes 53, 134–136 (2017). https://doi.org/10.1007/s11262-016-1390-2
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DOI: https://doi.org/10.1007/s11262-016-1390-2