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Characterization of Shuni viruses detected in Israel

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Abstract

Shuni virus (SHUV) was recently identified in Israel in several brains of ovine, bovine, and goat fetuses and newborn animals with congenital arthrogryposis-hydranencephaly syndrome. In the present study, the sequences of several Israeli SHUV strains were analyzed in detail; based on the small genome segment which encodes the nucleocapsid protein and the small nonstructural protein (NSs), a very high similarity of 99–100 % among each other was found. In contrast to the highly conserved N protein, several mutations were found within the NSs-coding sequence of SHUVs present in brain samples of malformed fetuses, resulting in a considerably frequent appearance of stop codons. Interferon alpha/beta production was demonstrated in an in-vitro interferon bioassay; hence, the virus isolated from the brain of a malformed sheep fetus acquired mutations, resulting in the loss of its NSs protein function.

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Acknowledgments

We are grateful to Nicolas Ruggli, Institute of Virology and Immunoprophylaxis, Mittelhäusern, for kindly providing the SK6-MxLUC cells, and Prof. Yehuda Stram, Dr. Anita Kovtunenko, and Mrs Anna Gorohov for their support.

Funding

This work was supported by the EU Twinning Project IL/10 CRIS/2015/356-293, Strengthening of Israeli veterinary diagnostic capacities, and funded in part by the EU Horizon 2020 program (European Commission Grant Agreement No. 643476 “COMPARE”).

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Authors and Affiliations

  1. Divisions of Virology, Kimron Veterinary Institute, 50250, Bet Dagan, Israel

    Natalia Golender, Velizar Bumbarov, Alexander Panshin & Yevgeny Khinich

  2. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493, Greifswald-Insel Riems, Germany

    Kerstin Wernike, Andrea Aebischer, Maria Jenckel & Martin Beer

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  1. Natalia Golender
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  2. Kerstin Wernike
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Correspondence to Kerstin Wernike.

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The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed. The permit number for the intracerebral injection of diagnostic material in mice is 020_b7650 from 21st of January 2014 (Diagnosis of Animal Disease caused by ARBOVIRUSES).

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Edited by Juergen A Richt.

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Golender, N., Wernike, K., Bumbarov, V. et al. Characterization of Shuni viruses detected in Israel. Virus Genes 52, 806–813 (2016). https://doi.org/10.1007/s11262-016-1381-3

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  • DOI: https://doi.org/10.1007/s11262-016-1381-3

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