Abstract
The incidence of squash leaf curl disease and molecular characterization of the Palestinian isolate of Squash leaf curl virus [SLCV–(PAL)] are described in this study. Symptomatic leaf samples obtained from squash (Cucurbita pepo), watermelon [Citrullus lanatus (Thunb.)], and cucumber (Cucumis sativus L.) plants were tested for SLCV-[PAL] infection by PCR and RCA. SLCV was also found to occur naturally in Chenopodium murale, Convolvulus sp, and Prosporis farcta which showed yellowing. The disease incidence was 85 % in samples collected from Nablus in summer season, while it was 98 % in samples collected from Qalqilia in autumn. On the other hand, SLCV incidence did not exceed 25 % in winter season. The full-length DNA-A and DNA-B genomes of SLCV-[PAL] were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis reveals that SLCV-[PAL] is closely related to other isolates from Lebanon (SLCV-LB2), Jordan (SLCV-JO), Israel (SLCV-IL), and Egypt (SLCV-EG). DNA-A of SLCV-[PAL] showed the highest nucleotide identity (99.4 %) with SLCV-JO, and SLCV-LB2, while DNA-B had the highest nucleotide identity (99.3 %) with SLCV-IL. However, following genome sequencing, it was found that due to two separate point mutations, two viral open reading frames (ORF) were altered in some SLCV Palestinian isolates. The AC2 ORF was extended by 141 nucleotides, while the AC4 ORF was extended by 36 nucleotides.
Similar content being viewed by others
References
S. Lazarowitz, Crit. Rev. Plant Sci. 11, 327–349 (1992)
J. Stanley, Semin. Virol. 2, 139–150 (1991)
J. Laufs, W. Traut, F. Heyraud, V. Matzeit, S.G. Rogers, J. Schell, B. Gronenborn, Proc. Natl. Acad. Sci. USA. 92, 3879–3883 (1995)
D.C. Stenger, G.N. Revington, M.C. Stevenson, D.M. Bisaro, Proc. Natl. Acad. Sci. USA. 88, 8029–8033 (1991)
J.E. Seal, F. van den Bosch, M.J. Jeger, Plant Sci. 25, 23–46 (2006). ISSN:0735-2689
A. Varma, V.G. Malathi, Ann. Appl. Biol. 142, 145–164 (2003)
International Committee on Taxonomy of Viruses (ICTV) http://ictvonline.org/virusTaxonomy.asp?taxnode_id=20120007. Accessed 21 Sep 2013
L. Hanley-Bowdoin, E.R. Bejarano, D. Robertson, S.H. Mansoor, Nat. Rev. Microbiol (2013). doi:10.1038/nrmicro3117
S.P. Tiwari, S. Nema, M.N. Khare, ESci J. Plant Pathol. 02(02), 102–120 (2013)
G.C. Wisler, J.E. Duffus, H.Y. Liu, R.H. Li, Plant Dis. 82, 270–280 (1998)
J. Navas-Castillo, E. Fiallo-Oliv’e, E.S. S’nchez-Campos, Annu. Rev. Phytopathol. 49, 219–248 (2011)
R.A. Flock, D.E. Mayhew, Plant Dis. 65, 75–76 (1981)
S. Cohen, J.E. Duffus, R.C. Larsen, H.Y. Liu, R.A. Flock, Phytopathology 73, 1669–1673 (1983)
Y. Antignus, O. Lachman, M. Pearlsman, S. Omer, H. Yunis, Y. Messika, O. Uko, A. Koren, Phytoparasitica 31, 415 (2003)
A.M. Idris, A. Abedl-Salam, J.K. Brown, Plant Dis. 90, 1262 (2006)
A. Al-Musa, G. Anfoka, S. Misbeh, M. Abhary, F.H. Ahmad, Phytopathology 156, 311–316 (2008)
M.S. Ali-Shtayeh, R.M. Jamous, E.Y. Husein, M.Y. Alkhader, Plant Dis. 94(5), 640 (2010)
H. Sobh, J. Samsatly, M. Jawhari, C. Najjar, A. Haidar, Y. Abou-Jawdah, Plant Dis. 96(8), 1231 (2012)
S.L. Dellaporta, J. Wood, J.B. Hicks, Plant Mol. Biol. Rep. I(4), 19–21 (1983)
M.R. Rojas, R.L. Gilbertson, D.R. Russell, D.P. Maxwell, Plant Dis. 77, 340–347 (1993)
M. R. Green, J. Sambrook, (Cold Springer Harbor Press, 2012), ISBN: 978-1-936113-42-2
American Phytopathological Society (2007). Whitefly spreads emerging plant viruses. Science Daily, http://www.sciencedaily.com/releases/2007/01/070118161622.htm. Accessed 24 Dec 2012
B.D. Harrison, D.J. Robinson, Annu. Rev. Phytopathol. 37, 369–398 (1999)
D.R. Jones, Eur. J. Plant Pathol. 109, 195–219 (2003)
A.K. Inoue-Nagata, L.C. Albuquerque, W.B. Rocha, T. Nagata, J. Virol. Methods 116, 209–211 (2004)
D. Haible, S. Kober, H. Jeske, J. Virol. Methods 135, 9–16 (2006)
S. Davino, C. Napoli, M. Davino, G. Accotto, Eur. J. Plant Pathol. 114, 293–299 (2006)
D. Knierim, E. Maiss, Arch. Virol. 152, 941–954 (2007)
B. Owor, D. Shepherd, N. Taylor, R. Edema, A. Monjane, J. Thomson, D. Martin, A. Varsani, J. Virol. Methods 140, 100–105 (2007)
C.M. Fauquet, R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X. Zhou, Arch. Virol. 153, 783–821 (2008)
A. Abudy, T. Sufrin-Ringwald, C. Dayan-Glick, D. Guenoune-Gelbart, O. Livneh, M. Zaccai, M. Lapidot, Israel J. Plant Sci. 58, 33–42 (2010). doi:10.1560/IJPS.58.1.33
S.E. Albrechtsen, Testing methods for seed transmitted viruses: Principles and protocols (CABL Publishing Oxfordshire, UK, 2006), p. 259
L. Hanley-Bowdoin, S.B. Settlage, B.M. Orozco, S. Nagar, D. Robertson, Crit. Rev. Plant Sci. 18, 71–106 (1999)
I. Amin et al., Mol. Plant Microbe Interact. 24, 973–983 (2011)
J. Zhang, J. Dong, Y. Xu, J. Wu, Virus Res. 163, 51–58 (2012)
Acknowledgments
This research was funded by the German Research Council’s trilateral (German-Israeli-Palestinian ref. no. JE 116/12-2) Project and by USAIDMERC Grant no. M26-063.
Author information
Authors and Affiliations
Corresponding author
Additional information
M. S. Ali-Shtayeh and R. M. Jamous have contributed equally to this study.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Ali-Shtayeh, M.S., Jamous, R.M., Hussein, E.Y. et al. Squash leaf curl virus (SLCV): a serious disease threatening cucurbits production in Palestine. Virus Genes 48, 320–328 (2014). https://doi.org/10.1007/s11262-013-1012-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-013-1012-1