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Full-length sequence analysis of hepatitis E virus isolates: showing potential determinants of virus genotype and identity

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Abstract

The complete genome sequence of a genotype 4 strain of hepatitis E virus (CH-YT-HEV02) from a patient (in Yantai, China) has been determined. Phylogenetic analysis showed that CH-YT-HEV02 belongs to genotype 4, subtype 4a. However, the phylogenetic analysis indicated that it was most closely related to JKO-CHiSai98C (AB197673) strain, sharing only 91.6 % sequence identity with it. Judging from the phylogenetic tree based on the full-length nucleotide sequences of all 70 genotype 4 HEV isolates retrieved from GenBank up to May, 2013, the CH-YT-HEV02 isolates could serve as a Yantai-indigenous strain. A broader comparison with other genotype isolates revealed that there are a few conserved amino acids in the HVR region of different HEV genotypes, and two amino acid motifs in ORF2 and ORF3 might serve as signatures of genotype diversity of HEV.

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Acknowledgments

This study was supported by a grant from the National Natural Science Foundation of China (No. 30930078).

Conflict of interest

The authors declare no competing financial interests.

Ethical standards

The experimental protocols were approved by the Animal Care and Protection Committee of Institute of Health and Environmental Medicine.

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Authors and Affiliations

  1. Department of Environment and Health, Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, No. 1, Dali Road, Tianjin, 300050, China

    Dong Yang, Min Jin, Zhigang Qiu, Zhiqiang Shen, Zhaoli Chen, Xinwei Wang & Jun-Wen Li

  2. Yantai Center for Disease Control and Prevention, No. 17, Fuhou Road, Yantai, 264003, Shandong, China

    Mei Jiang, Weihong Cui, Bo Li & Lianfeng Gong

Authors
  1. Dong Yang
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  2. Mei Jiang
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  3. Min Jin
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  10. Xinwei Wang
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  11. Jun-Wen Li
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Corresponding author

Correspondence to Jun-Wen Li.

Additional information

Dong Yang and Mei Jiang contributed equally to this study.

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Yang, D., Jiang, M., Jin, M. et al. Full-length sequence analysis of hepatitis E virus isolates: showing potential determinants of virus genotype and identity. Virus Genes 47, 414–421 (2013). https://doi.org/10.1007/s11262-013-0962-7

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  • DOI: https://doi.org/10.1007/s11262-013-0962-7

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