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The genome of a baculovirus isolated from Hemileuca sp. encodes a serpin ortholog

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Abstract

The genome sequence of a baculovirus from Hemileuca sp. was determined. The genome is 140,633 kb, has a G+C content of 38.1 %, and encodes 137 putative open-reading frames over 50 amino acids. 126 of these ORFs showed similarity to other baculovirus genes in the database including all 37 core genes. Of the remaining 11 predicted genes, one is related to a lepidopteran serpin gene. This is the first report of a baculovirus encoding a member of this family of serine protease inhibitors, and to our knowledge the first report of a viral serpin outside the Poxviridae. The genome also contained three homologous repeat sequences. Phylogenetic analysis indicated that the virus is a group II Alphabaculovirus and belongs to a lineage that includes Orgyia leucostigma, Ectropis obliqua, Apocheima cinerarium, and Euproctis pseudoconspersa nucleopolyhedroviruses.

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Acknowledgments

The research was supported by a Tartar Grant to GR and a Canadian Crop Genomics Initiative grant to ME and DT. The technical assistance of Doug Baldwin in the preparation of HespNPV DNA for sequencing and the assembly of the initial virus sequence is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Microbiology, Oregon State University, Corvallis, OR, 97331-3804, USA

    George F. Rohrmann

  2. Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada

    Martin A. Erlandson

  3. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, BC, V0H 1Z0, Canada

    David A. Theilmann

Authors
  1. George F. Rohrmann
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  2. Martin A. Erlandson
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  3. David A. Theilmann
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Correspondence to George F. Rohrmann.

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Rohrmann, G.F., Erlandson, M.A. & Theilmann, D.A. The genome of a baculovirus isolated from Hemileuca sp. encodes a serpin ortholog. Virus Genes 47, 357–364 (2013). https://doi.org/10.1007/s11262-013-0951-x

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  • DOI: https://doi.org/10.1007/s11262-013-0951-x

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