Abstract
Yellow mosaic disease causes severe yield loss in grain legumes in Indian subcontinent and south east Asia. The disease is caused by two virus species, Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV). They have genome organization typical of Old World begomoviruses, the unique feature being the presence of an open reading frame (ORF) AV2 upstream of coat protein gene. In order to elucidate its function, ORF AV2 of blackgram isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Blackgram 3:1991] MYMIV-[IN:ND:Bg3:91] and cowpea isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Cowpea7:1998] MYMIV-[IN:ND:Cp7:98], respectively, were over expressed in Escherichia coli in fusion with maltose binding protein (MBP). The recombinant protein did not show efficient binding to DNA. However, both MBP-BgAV2 and MBP-CpAV2 proteins modulated nicking and ATPase activity of replication initiation protein (Rep). Even low concentration, 20 ng of MBP-BgAV2 and MBP-CpAV2 could bring 20 folds increase in nicking activity of Rep. Similarly in the presence of AV2 protein, two to three fold increase in ATPase activity was observed. It is hypothesized that AV2 protein may play a role of accessory protein modulating Rep activities.
Similar content being viewed by others
References
K.S. Usharani, M. Periasamy, V.G. Malathi, Virus Res. 119, 154–162 (2006)
A. Varma, A.K. Dhar, B. Mandal in Proceedings of international workshop, Bangkok (Asian Vegetable Research and Development Center, Taipei, 1992) pp. 8–27
C.M. Fauquet, R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X. Zhou, Arch. Virol. 153, 783–821 (2008)
M. Padidam, R.N. Beachy, C.M. Fauquet, Virology 224, 390–404 (1996)
M.I. Boulton, D.I. King, J. Donson, J.W. Davies, Virology 183, 114–121 (1991)
M.R. Rojas, H. Jiang, R. Salati, B. Xoconostile-Cazares, M.R. Sudarshana, W.J. Lucas, R.L. Gilbertson, Virology 291, 110–125 (2001)
S.G. Hormuzdi, D.M. Bisaro, Virology 193, 900–909 (1993)
J. Stanley, J.R. Latham, M.S. Pinner, I. Bedford, P.G. Markham, Virology 191, 396–405 (1992)
A. Zrachya, E. Glick, Y. Levy, T. Arazi, V. Citovsky, Y. Gafni, Virology 358, 159–165 (2007)
R.V. Chowda-Reddy, F. Achenjang, C. Felton, M.T. Etarock, M.T. Anangfac, P. Nugent, V.N. Fondong, Virus Res. 135, 115–124 (2008)
V. Pant, D. Gupta, N.R. Choudhury, V.G. Malathi, A. Varma, S.K. Mukherjee, J. Gen. Virol. 83, 1559–2567 (2001)
J. Sambrook, D.W. Russel, Molecular cloning, a laboratory manual, 3rd edn. (Cold spring Harbor Laboratory Press, New York, 2001)
P.S. Malik, V. Kumar, B. Bagewadi, S.K. Mukherjee, Virology 337, 273–283 (2005)
B. Bagewadi, S. Chen, S. Lal, N.R. Choudhury, S.K. Mukherjee, J. Virol. 78, 11890–11903 (2004)
A. Rouhibakhsh, Q.M.I. Haq, V.G. Malathi, J. Biosci. 36, 329–340 (2011)
G. Kotlizky, M.I. Boulton, C. Pitaksutheepong, J.W. Davies, B.L. Epel, Virology 274, 32–38 (2000)
S. Karjee, M.N. Islam, S.K. Mukherjee, Meth. Mol. Biol. 442, 187–203 (2008)
Acknowledgements
We are grateful to Dr HS Gupta, Director, IARI, and Dr RK Jain, Head, Div. of Plant Pathology for providing the infrastructural support in the present investigation. We thank the scientists and staff at National Phytotron Facility, IARI for their support and guidance to grow plants under controlled conditions. The financial support given by Department of Biotechnology, Govt. of India (Functional genomics of yellow mosaic viruses of soybean and development of transgenic resistance in soybean: BT/PR9631/AGR/02/468/2007) is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rouhibakhsh, A., Choudhury, N.R., Mukherjee, S.K. et al. Enhanced nicking activity of Rep in presence of pre-coat protein of Mungbean yellow mosaic India virus. Virus Genes 44, 356–361 (2012). https://doi.org/10.1007/s11262-011-0701-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-011-0701-x