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Characterization of H5N1 highly pathogenic avian influenza viruses isolated from poultry in Pakistan 2006–2008

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Abstract

Nine avian influenza viruses (AIV), H5N1 subtype, were isolated from dead poultry in the Karachi region of Pakistan from 2006 to 2008. The intravenous pathogenicity indices and HA protein cleavage sites of all nine viruses were consistent with highly pathogenic AIV. Based on phylogenetic analysis of the HA genes, these isolates belong to clade 2.2 and both the HA and NA are closely related to each other (nucleotide identities above 99.0%) and to other Middle Eastern H5N1 AIV isolates (nucleotide identities above 98.0%). The phylogenetic data suggest that the virus in both epornitics of H5N1 HPAIV in commercial poultry in the Karachi region of Pakistan between 2006 and 2008 were from a very closely related source, however, there is inadequate epidemiological data to determine what the reservoir was for the virus between the 2006 and 2007 outbreaks other than that there was a single introduction into the region.

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References

  1. K. Naeem, The Avian Influenza H7N3 Outbreak in South Central Asia. 4th international symposium on avian influenza, Athens, GA, 1997, pp. 31–35

  2. K. Naeem, M. Hussain, Vet. Rec. 137, 439 (1995)

    Article  PubMed  CAS  Google Scholar 

  3. K. Naeem, N. Siddique, M. Ayaz, M.A. Jalalee, Avian Dis. 51, 189–193 (2007)

    Article  PubMed  CAS  Google Scholar 

  4. K. Naeem, A. Ullah, R.J. Manvell, D.J. Alexander, Vet. Rec. 145, 560 (1999)

    Article  PubMed  CAS  Google Scholar 

  5. OIE, Avian influenza: home page. World Organization for Animal Health, 2008

  6. WHO, Human cases of avian influenza A (H5N1) in north-west frontier province, Pakistan, October–November 2007. Wkly Epidemiol Rec, Geneva, 2008, pp. 359–364

  7. D.E. Swayne, D. Senne, D.L. Suarez, in Isolation and Identification of Avian Pathogens, ed. by L. Dufour-Zavala, D.E. Swayne, J. Glisson, J. Pearson, W. Reed, M. Jackwood, P. Woolcock (American Association of Avian Pathologists, Jacksonville, 2008), pp. 128–134

    Google Scholar 

  8. D.L. Suarez, M. Garcia, J. Latimer, D. Senne, M. Perdue, J. Virol. 73, 3567–3573 (1999)

    PubMed  CAS  Google Scholar 

  9. K. Tamura, J. Dudley, M. Nei, S. Kumar, Mol. Biol. Evol. 24, 1596–1599 (2007)

    Article  PubMed  CAS  Google Scholar 

  10. M. Kimura, J. Mol. Evol. 16, 111–120 (1980)

    Article  PubMed  CAS  Google Scholar 

  11. D.A. Senne, B. Panigrahy, Y. Kawaoka, J.E. Pearson, J. Suss, M. Lipkind, H. Kida, R.G. Webster, Avian Dis. 40, 425–437 (1996)

    Article  PubMed  CAS  Google Scholar 

  12. H. Chen, Y. Li, Z. Li, J. Shi, K. Shinya, G. Deng, Q. Qi, G. Tian, S. Fan, H. Zhao, Y. Sun, Y. Kawaoka, J. Virol. 80, 5976–5983 (2006)

    Article  PubMed  CAS  Google Scholar 

  13. G. Wang, D. Zhan, L. Li, F. Lei, B. Liu, D. Liu, H. Xiao, Y. Feng, J. Li, B. Yang, Z. Yin, X. Song, X. Zhu, Y. Cong, J. Pu, J. Wang, J. Liu, G.F. Gao, Q. Zhu, J. Gen. Virol. 89, 697–702 (2008)

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge Joan Beck, Joyce Bennett and Melissa Scott at Southeast Poultry Research Laboratory for technical assistance. Funding was provided by the United States Department of State Biosecurity Engagement Program through a Memorandum of Agreement with the United States Department of Agriculture, Agricultural Research Service.

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Authors and Affiliations

  1. Southeast Poultry Research Laboratory, USDA-Agricultural Research Service, 934 College Station Rd., Athens, GA, 30605, USA

    Jamie L. Wasilenko, Mary Pantin-Jackwood, David E. Swayne & Erica Spackman

  2. Sindh Poultry Vaccine Center, Animal Science Complex, Korangi, Karachi, Pakistan

    Taseer Ahmed Khan, Ayaz Ahmed, Shafqat F. Rehmani & Nazir Ahmed Lone

  3. Department of Physiology, University of Karachi, Karachi, 75270, Pakistan

    Taseer Ahmed Khan

Authors
  1. Jamie L. Wasilenko
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  2. Mary Pantin-Jackwood
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  3. Taseer Ahmed Khan
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  4. Ayaz Ahmed
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  5. Shafqat F. Rehmani
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  6. Nazir Ahmed Lone
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  7. David E. Swayne
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  8. Erica Spackman
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Correspondence to Erica Spackman.

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Wasilenko, J.L., Pantin-Jackwood, M., Khan, T.A. et al. Characterization of H5N1 highly pathogenic avian influenza viruses isolated from poultry in Pakistan 2006–2008. Virus Genes 44, 247–252 (2012). https://doi.org/10.1007/s11262-011-0689-2

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  • DOI: https://doi.org/10.1007/s11262-011-0689-2

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