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Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus

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Abstract

Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and disease symptoms were reproduced after experimental aphid transmission. The complete genome of TVDV was determined from cloned RT-PCR products derived from viral RNA. It was 5,920 nucleotides (nts) long and had the six major open reading frames (ORFs) typical of a member of the genus Polerovirus. Sequence comparisons showed that it differed significantly from any of the other species in the genus and this was confirmed by phylogenetic analyses of the RdRp and coat protein. SDS-PAGE analysis of purified virions gave two protein bands of about 26 and 59 kDa both of which reacted strongly in Western blots with antiserum produced to prokaryotically expressed TVDV CP showing that the two forms of the TVDV CP were the only protein components of the capsid.

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Acknowledgments

This work was supported continuously by the Research and Development Foundation of the Yunnan Tobacco Company (04A18, 07A07). We are grateful to Sunson Enzymes, China, and Mr. Shaofei Yang for generously providing all the enzymes used in virus purifications, to Prof. Zhongkai Zhang, Yunnan Academy of Agricultural Science, for electron microscopy, and to Dr H.-H. Steinbiss, MPI, Cologne, Germany for kindly providing the pSBET expression vector. We also thank Prof. M.J. Adams, Rothamsted Research, Harpenden, Herts, UK for correcting the English of the manuscript. Xiaohan Mo is a graduate student registered in the College of Life Sciences, Zhejiang University.

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, China

    Xiao-han Mo & Jian-ping Chen

  2. Yunnan Academy of Tobacco Agricultural Sciences, 14 Nanxiang Road, Yuxi, 653100, China

    Xiao-han Mo & Zheng-bin Chen

  3. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou, 310021, China

    Jian-ping Chen

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  1. Xiao-han Mo
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  2. Zheng-bin Chen
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Correspondence to Jian-ping Chen.

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Mo, Xh., Chen, Zb. & Chen, Jp. Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus . Virus Genes 41, 425–431 (2010). https://doi.org/10.1007/s11262-010-0524-1

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  • DOI: https://doi.org/10.1007/s11262-010-0524-1

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