Abstract
The tri-segmented RNA genome of hantaviruses facilitates genetic reassortment by segment swapping when cells are co-infected with different virus strains. We found efficient in vitro reassortment between members of two different genetic lineages of the Dobrava-Belgrade virus species, the weakly virulent DOBV-Aa and highly virulent DOBV-Af. In all reassortants, S and L segments originated from the same parental strain, and only the M segment was exchanged. To identify functional differences between the parental strains DOBV-Aa and DOBV-Af in cell culture and to compare them with the reassortants, we studied elements of the innate immunity in virus-infected cells. The contrasting phenotypes of the parental viruses were maintained by the reassortants carrying the respective S and L segments of the parental virus and were not influenced by the origin of the M segment.
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Acknowledgments
We thank Dr. Martin Raftery for critical reading of the manuscript. SK and MHL were graduates funded by Deutsche Forschungsgemeinschaft (DFG) in the Research Training group 1121. Parts of the work were supported by additional DFG grants (KR1293/9-1 and RA1057/2-1).
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Kirsanovs, S., Klempa, B., Franke, R. et al. Genetic reassortment between high-virulent and low-virulent Dobrava-Belgrade virus strains. Virus Genes 41, 319–328 (2010). https://doi.org/10.1007/s11262-010-0523-2
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DOI: https://doi.org/10.1007/s11262-010-0523-2