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Genomic characterization of swine caliciviruses representing a new genus of Caliciviridae

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Abstract

This study reports the molecular characterization of novel caliciviruses, the St-Valérien-like viruses, which were isolated from pig feces in the province of Quebec, Canada between 2005 and 2007. The genomes of St-Valérien-like viruses contain 6409 nucleotides and include two main open reading frames (ORFs). ORF1 encodes the non structural (NS) polyprotein and the major capsid protein (VP1) while ORF2 encodes the putative basic minor capsid protein. Typical conserved amino acid motifs predict a gene order reminiscent of calicivirus genomes. Phylogenetic, pairwise homology, and distance analyses performed on complete genomic sequences and partial amino acid sequences from the NTPase, polymerase, and major capsid protein segregated the St-Valérien-like viruses in a unique cluster sharing a common root with the Tulane virus and the noroviruses. Based on the genomic analyses presented, the St-Valérien-like viruses are members of a new genus of Caliciviridae for which we propose the name Valovirus.

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Acknowledgments

The authors would like to thank Marion Koopmans, Harry Vennema, and Kim Green for helpful discussions and critical review of the manuscript. Special thanks to Denis L’Homme for enlightening discussions on phylogenies. This work was supported by the science division of the CFIA/ACIA.

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Authors and Affiliations

  1. St-Hyacinthe Laboratory, Canadian Food Inspection Agency, 3400 Blvd Casavant West, St-Hyacinthe, QC, J2S 8E3, Canada

    Yvan L’Homme, Raphaël Sansregret, Étienne Plante-Fortier, Anne-Marie Lamontagne, Mourad Ouardani, Geneviève Lacroix & Carole Simard

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  1. Yvan L’Homme
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Correspondence to Yvan L’Homme.

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L’Homme, Y., Sansregret, R., Plante-Fortier, É. et al. Genomic characterization of swine caliciviruses representing a new genus of Caliciviridae . Virus Genes 39, 66–75 (2009). https://doi.org/10.1007/s11262-009-0360-3

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