Skip to main content

Advertisement

SpringerLink
Log in

Molecular characterization of an Indian isolate of Banana bunchy top virus based on six genomic DNA components

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Banana bunchy top virus (BBTV) is a single-stranded circular DNA virus of the genus Babuvirus, belonging to family Nanoviridae. The six genomic DNA components of Indian (Lucknow) isolate of BBTV were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of banana bunchy top disease (BBTD). The resulting ~1.1 Kb amplicons were cloned and sequenced. Analysis of sequence data revealed the presence of six full-length components of BBTV: DNA-R (1111 bp), DNA-U3 (1060 bp), DNA-S (1075 bp), DNA-M (1048 bp), DNA-C (1018 bp), and DNA-N (1096 bp). Comparisons of sequence data of the six DNA components of the BBTV Lucknow isolate revealed highest identities with sequences of other BBTV isolates from the South Pacific group: [DNA-R (98%), DNA-U3 (93%), DNA-S (100%), DNA-M (98%), DNA-C (97%), and DNA-N (99%)]. A phylogenetic analysis revealed a close relationship of the Lucknow isolate with BBTV isolates of South Pacific group rather than those of the Asian group. Based on these analyses the virus has been classified as BBTV Lucknow, a new member of South Pacific group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. J.L. Dale, Adv. Virus Res. 33, 301–325 (1987). doi:https://doi.org/10.1016/S0065-3527(08)60321-8

    Article  CAS  Google Scholar 

  2. C.W. Wardlaw, Banana Diseases, Including Plantains and Abaca (William Clowes and Sons Ltd. London, Beccles and Colchester, 1972), pp. 68–115

    Google Scholar 

  3. R.M. Harding, T.M. Burns, J.L. Dale, J. Gen. Virol. 72, 225–230 (1991). doi:https://doi.org/10.1099/0022-1317-72-2-225

    Article  CAS  Google Scholar 

  4. J.E. Thomas, R.G. Dietzgen, J. Gen. Virol. 72, 217–224 (1991). doi:https://doi.org/10.1099/0022-1317-72-2-217

    Article  CAS  Google Scholar 

  5. R.Y. Wu, H.J. Su, J. Phytopathol. 128, 153–160 (1990). doi:https://doi.org/10.1111/j.1439-0434.1990.tb04261.x

    Article  Google Scholar 

  6. H.J. Vetten, P.W. Chu, J.L. Dale, R.M. Harding, J. Hu, L. Katul, M. Kojima, J.W. Randles, Y. Sano, J.E. Thomas, in Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Academic Press, San Diego, London, 2005), pp. 343–352

    Google Scholar 

  7. T.M. Burns, R.M. Harding, J.L. Dale, J. Gen. Virol. 76, 1471–1480 (1995). doi:https://doi.org/10.1099/0022-1317-76-6-1471

    Article  CAS  Google Scholar 

  8. R.P. Beetham, G.J. Hafner, R.M. Harding, J.L. Dale, J. Gen. Virol. 78, 229–236 (1997)

    Article  CAS  Google Scholar 

  9. M. Karan, R.M. Harding, J.L. Dale, J. Gen. Virol. 75, 3541–3546 (1994). doi:https://doi.org/10.1099/0022-1317-75-12-3541

    Article  CAS  Google Scholar 

  10. M. Karan, R.M. Harding, J.L. Dale, Molecular Plant Pathology on-line (1997), http://www.bspp.org.uk/mppol/1997/0624karan. Accessed 30 Jan 2009

  11. T.E. Zhuang Jun, L. Zhi-Xin, Chin, J. Agric. Biotechnol. 2, 91–97 (2005). doi:https://doi.org/10.1079/CJB200566

    Google Scholar 

  12. N. Furuya, T.O. Dizon, K.T. Natsuaki, J. Gen. Plant Pathol. 71, 68–73 (2005). doi:https://doi.org/10.1007/s10327-004-0162-3

    Article  CAS  Google Scholar 

  13. N. Furuya, T.O. Dizon, K.T. Natsuaki, J. Agric. Sci. 51, 92–101 (2006)

    CAS  Google Scholar 

  14. I. Amin, J. Qazi, S. Mansoor, M. Ilyas, R.W. Briddon, Virus Genes 36, 191–198 (2008). doi:https://doi.org/10.1007/s11262-007-0168-y

    Article  CAS  Google Scholar 

  15. M. Sharman, J.E. Thomas, S. Skabo, T.A. Holton, Arch. Virol. 153, 135–147 (2008). doi:https://doi.org/10.1007/s00705-007-1077-z

    Article  CAS  Google Scholar 

  16. F.A.O.S.T.A.T. Anonymous, ProdSTAT: Crops. UN Food and Agriculture Organisation (2008), p. 567, (http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567). Accessed 19 Feb 2007

  17. S.L. Dellaporta, J. Woods, T.B. Hicks, Plant Mol. Biol. Rep. 1, 19–21 (1983). doi:https://doi.org/10.1007/BF02712670

    Article  CAS  Google Scholar 

  18. R.M. Harding, G.J. Hafner, R.G. Dietzgen, J.L. Dale, J. Gen. Virol. 74, 323–328 (1993). doi:https://doi.org/10.1099/0022-1317-74-3-323

    Article  CAS  Google Scholar 

  19. S.F. Altschul, T.L. Madden, A.A. Schaffer, J. Zhang, W. Miller, D.J. Lipman, Nucleic Acids Res. 25, 3389–3402 (1997). doi:https://doi.org/10.1093/nar/25.17.3389

    Article  CAS  PubMed Central  Google Scholar 

  20. B. Morgenstern, K. Frech, A. Dress, T. Werner, Bioinformatics 14, 290–294 (1998). doi:https://doi.org/10.1093/bioinformatics/14.3.290

    Article  CAS  Google Scholar 

  21. S. Kumar, K. Tamura, I.B. Jakobsen, M. Nei, MEGA2: Molecular Evolutionary Genetics Analysis Software (Arizona State University, Arizona, USA, 2001)

    Google Scholar 

  22. V.A. Herrera-Valencia, B. Dugdale, R.M. Harding, J.L. Dale, J. Gen. Virol. 87, 3409–3412 (2006). doi:https://doi.org/10.1099/vir.0.82166-0

    Article  CAS  Google Scholar 

  23. T.M. Burns, R.M. Harding, J.L. Dale, Arch. Virol. 137, 371–380 (1994). doi:https://doi.org/10.1007/BF01309482

    Article  CAS  Google Scholar 

  24. A.E. Gorbalenya, E.V. Koonin, Y. Wolf, FEBS Lett. 262, 145–148 (1990). doi:https://doi.org/10.1016/0014-5793(90)80175-I

    Article  CAS  Google Scholar 

  25. P.R. Beetham, R.M. Harding, J.L. Dale, Arch. Virol. 144, 89–105 (1999). doi:https://doi.org/10.1007/s007050050487

    Article  CAS  Google Scholar 

  26. G.N. Revington, G. Sunter, D.M. Bisaro, Plant Cell 1, 985–992 (1987)

    Article  Google Scholar 

  27. K. Saunders, A. Lucy, J. Stanley, Nucleic Acids Res. 19, 2325–2330 (1991). doi:https://doi.org/10.1093/nar/19.9.2325

    Article  CAS  PubMed Central  Google Scholar 

  28. F. Heyraud, V. Matzeit, M. Kammann, S. Schaefer, J. Schell, B. Gronenborn, EMBO J. 12, 4445–4452 (1993)

    Article  CAS  PubMed Central  Google Scholar 

  29. T. Timchenko, F. Kouchkovsky, L. Katul, C. David, H.J. Vetten, B. Gronenborn, J. Virol. 73, 10173–10182 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  30. J.-M. Hu, H.-C. Fu, C.-H. Lin, H.-J. Su, H.-H. Yeh, J. Virol. 81, 1746–1761 (2007). doi:https://doi.org/10.1128/JVI.01390-06

    Article  CAS  Google Scholar 

  31. K. Manickam, S. Doraiswamy, T. Ganapathy, G. Thiribhuvana, R. Rabindran, Z. Pflanzenkr, Pflanzenschutz 108, 490–499 (2001)

    CAS  Google Scholar 

Download references

Acknowledgments

We thank the Director, National Botanical Research Institute, Lucknow for providing facilities and keen interest, and the University Grant Commission (UGC), New Delhi for providing fellowship to one of the authors namely R. Vishnoi.

Author information

Authors and Affiliations

  1. Plant Molecular Virology Lab, National Botanical Research Institute, Lucknow, 226 001, India

    Radha Vishnoi & Shri Krishna Raj

  2. Department of Botany, Molecular Plant Virology Lab, Lucknow University, Lucknow, 226 007, India

    Vivek Prasad

Authors
  1. Radha Vishnoi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shri Krishna Raj
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vivek Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shri Krishna Raj.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vishnoi, R., Raj, S.K. & Prasad, V. Molecular characterization of an Indian isolate of Banana bunchy top virus based on six genomic DNA components. Virus Genes 38, 334–344 (2009). https://doi.org/10.1007/s11262-009-0331-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-009-0331-8

Keywords

Search

Navigation