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Characterization of the genes encoding complete US10, SORF3, and US2 proteins from duck enteritis virus

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Abstract

Based on the understanding that the analysis on unique short (US) region of duck enteritis virus (DEV) might contribute to the recognition of the molecular characterization and the evolution of DEV, in the study, a 5,121 bp fragment, which contained three genes encoding complete US10, unique short region open reading frame (SORF) 3, and US2 proteins, was amplified from the DEV (C-KCE) genome. The transcription orientation of the US10 and SORF3 was in a tail-to-tail way, and the SORF3 and US2 was the same. Potential core promoters and polyadenylation (poly(A)) sites were predicted for US10, SORF3, and US2 and further confirmed by polymerase chain reaction. Phylogenetic analysis for the three complete coding sequences showed that DEV was more closely related to avian herpesviruses, especially to Mardivirus, and should be classified to a separate genus of the Alphaherpesvirinae subfamily.

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Acknowledgments

We thank Prof. Ma LX for kindly providing plasmid pHBM803. This research was supported by Research Fund for the Doctoral Program of Higher Education of China (20070511004).

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Authors and Affiliations

  1. Division of Animal Pathogens, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People’s Republic of China

    Yong Hu, Hongbo Zhou, Zhengjun Yu, Huanchun Chen & Meilin Jin

  2. State Key Laboratory of Agricultural Microbiology, Laboratory of Animal Infectious Diseases, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, 430070, Wuhan, Hubei, People’s Republic of China

    Hongbo Zhou, Zhengjun Yu, Huanchun Chen & Meilin Jin

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  1. Yong Hu
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Correspondence to Meilin Jin.

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Hu, Y., Zhou, H., Yu, Z. et al. Characterization of the genes encoding complete US10, SORF3, and US2 proteins from duck enteritis virus. Virus Genes 38, 295–301 (2009). https://doi.org/10.1007/s11262-009-0329-2

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  • DOI: https://doi.org/10.1007/s11262-009-0329-2

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