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Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts

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Abstract

Infectious bursal disease virus (IBDV) causes immunosuppression in chickens. We investigated the molecular changes in chicken embryo fibroblasts (CEF) adapted IBDV by genomic sequencing. IBDV were serially passaged in CEF and chickens were infected with the IBDV obtained after different numbers of passages in CEF. Chicken infections showed that 16th, 20th, and 21st passage viruses were pathogenic, while 26th and 36th passage viruses were non-pathogenic. Sequencing demonstrated that the initial changes during the serial passage comprised of a single-nucleotide deletion in the 3′ non-coding region of segment B of the virus after 19th passage, followed by changes in the VP1 gene after the 20th passage of the virus and changes in VP2, VP5 after the 21st passage of the virus. These data suggested that the attenuation of very virulent IBDV was due to multigenic mutations and there are in vitro and in vivo competitive replications in IBDV quasispecies.

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References

  1. P. Dobos, B.J. Hill, R. Hallett, D.T. Kells, H. Becht, D. Teninges, J. Virol. 32, 593–605 (1979)

    CAS  PubMed  PubMed Central  Google Scholar 

  2. J.M. Sharma, I.J. Kim, S. Rautenschlein, H.Y. Yeh, Dev. Comp. Immunol. 24, 223–235 (2000). doi:https://doi.org/10.1016/S0145-305X(99)00074-9

    Article  CAS  Google Scholar 

  3. E. Mundt, J. Beyer, H. Muller, J. Gen. Virol. 76(Pt 2), 437–443 (1995). doi:https://doi.org/10.1099/0022-1317-76-2-437

    Article  CAS  Google Scholar 

  4. P.J. Hudson, N.M. McKern, B.E. Power, A.A. Azad, Nucleic Acids Res. 14, 5001–5012 (1986). doi:https://doi.org/10.1093/nar/14.12.5001

    Article  CAS  Google Scholar 

  5. U. Spies, H. Muller, H. Becht, Nucleic Acids Res. 17, 7982 (1989). doi:https://doi.org/10.1093/nar/17.19.7982

    Article  CAS  Google Scholar 

  6. N. Lejal, B. Da Costa, J.C. Huet, B. Delmas, J. Gen. Virol. 81, 983–992 (2000)

    Article  CAS  Google Scholar 

  7. E. Lombardo, A. Maraver, J.R. Cast n, J. Rivera, A. Fernandez-Arias, A. Serrano, J.L. Carrascosa, J.F. Rodriguez, J. Virol. 73, 6973–6983 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  8. M. Brandt, K. Yao, M. Liu, R.A. Heckert, V.N. Vakharia, J. Virol. 75, 11974–11982 (2001). doi:https://doi.org/10.1128/JVI.75.24.11974-11982.2001

    Article  CAS  Google Scholar 

  9. D.J. Jackwood, B. Sreedevi, L.J. LeFever, S.E. Sommer-Wagner, Virology 377, 110–116 (2008). doi:https://doi.org/10.1016/j.virol.2008.04.018

    Article  CAS  Google Scholar 

  10. H.J. Boot, A.A. ter Huurne, A.J. Hoekman, B.P. Peeters, A.L. Gielkens, J. Virol. 74, 6701–6711 (2000)

    Article  CAS  Google Scholar 

  11. X. Wang, H. Zhang, H. Gao, C. Fu, Y. Gao, Y. Ju, Virus Genes 34, 67–73 (2007). doi:https://doi.org/10.1007/s11262-006-0002-y

    Article  CAS  Google Scholar 

  12. K. Yao, V.N. Vakharia, Virology 285, 50–58 (2001). doi:https://doi.org/10.1006/viro.2001.0947

    Article  CAS  Google Scholar 

  13. H.J. Boot, A.J. Hoekman, A.L. Gielkens, Arch. Virol. 150, 137–144 (2005). doi:https://doi.org/10.1007/s00705-004-0405-9

    Article  CAS  Google Scholar 

  14. E. Domingo, J.J. Holland, Annu. Rev. Microbiol 51, 151–178 (1997). doi:https://doi.org/10.1146/annurev.micro.51.1.151

    Article  CAS  Google Scholar 

  15. P.R. Harrigan, S. Bloor, B.A. Larder, J. Virol. 72, 3773–3778 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  16. R.M. Troyer, K.A. Garver, J.C. Ranson, A.R. Wargo, G. Kurath. Virus Res. 2008. doi:https://doi.org/10.1016/j.virusres.2008.07.018

    Article  CAS  Google Scholar 

  17. P.D. Lukert, R.B. Davis, Avian Dis. 18, 243–250 (1974). doi:https://doi.org/10.2307/1589133

    Article  CAS  Google Scholar 

  18. S. Kumar, K. Tamura, M. Nei, Brief. Bioinform. 5, 150–163 (2004). doi:https://doi.org/10.1093/bib/5.2.150

    Article  CAS  Google Scholar 

  19. M.D. Brown, M.A. Skinner, Virus Res. 40, 1–15 (1996). doi:https://doi.org/10.1016/0168-1702(95)01253-2

    Article  CAS  Google Scholar 

  20. T. Yamaguchi, M. Ogawa, Y. Inoshima, M. Miyoshi, H. Fukushi, K. Hirai, Virology 223, 219–223 (1996). doi:https://doi.org/10.1006/viro.1996.0470

    Article  CAS  Google Scholar 

  21. P. Jiang, J.A. Faase, H. Toyoda, A. Paul, E. Wimmer, A.E. Gorbalenya, Proc. Natl. Acad. Sci. USA 104, 9457–9462 (2007). doi:https://doi.org/10.1073/pnas.0700451104

    Article  CAS  Google Scholar 

  22. J. Sewatanon, S. Srichatrapimuk, P. Auewarakul, Intervirology 50, 123–132 (2007). doi:https://doi.org/10.1159/000098238

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by National Key Basic Research Program of China (Grant 2005CB523203), National Key Technology Research and Development Program of China (Grant 2006BAD06A04), National Natural Science Foundation of China (Grant No. 30625030, 30870117), and State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The first Affiliated Hospital of Medical College, Zhejiang University (Grant No. 2008A03). We thank Prof. Jimmy Kwang for critical revision from Temasek Life Sciences Laboratory, The National University of Singapore.

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Authors and Affiliations

  1. Key Laboratory of Animal Epidemic Etiology and Immunological Prevention of Ministry of Agriculture, Zhejiang University, Hangzhou, 310029, People’s Republic of China

    Lixue Shi, Haibin Li, Guangpeng Ma, Jiyong Zhou, Lianlian Hong, Xiaojuan Zheng, Yongping Wu, Yongzhi Wang & Yan Yan

  2. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou, 310003, People’s Republic of China

    Lixue Shi, Haibin Li, Jiyong Zhou, Lianlian Hong, Xiaojuan Zheng, Yongping Wu, Yongzhi Wang & Yan Yan

  3. China Rural Technology Development Center, Beijing, 100045, People’s Republic of China

    Guangpeng Ma

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  1. Lixue Shi
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Correspondence to Jiyong Zhou.

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Shi, L., Li, H., Ma, G. et al. Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts. Virus Genes 39, 46–52 (2009). https://doi.org/10.1007/s11262-008-0313-2

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