Skip to main content
SpringerLink
Log in

Molecular characterization and phylogenetic analysis of the complete genome of a hepatitis E virus from European swine

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Hepatitis E virus (HEV) has been detected in humans and in a broad range of animals, including pigs. For the first time the full-length genomic sequence of a HEV of European porcine origin, termed swX07-E1, was determined. Comparative analysis of 76 complete or nearly complete nucleotide sequences showed that swX07-E1 shares the highest nucleotide identity with Japanese swine HEV swJ8-5 and swJ12-4. The whole-genome phylogenetic analysis showed that swX07-E1 from Europe belongs to genotype-3 HEV, clusters with variants from Japan, Mongolia and Kyrgyzstan in subgroup 3c, but it is divergent from the prototype US HEV. Our analysis indicates that swX07-E1 represents a new subgroup of genotype-3 and that analysis of full-length sequences is necessary to discover new subgroups of HEV. According to our knowledge, swX07-E1 is the first full-length genome sequence of HEV from European swine. Knowledge about the full length HEV sequence from European swine is very important for understanding the HEV evolutionary events and the molecular mechanism of infection in human and in animals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. S.U. Emerson, D. Anderson, A. Arankalle, X.J. Meng, M. Purdy, G.G. Schlauder et al., in Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Elsevier/Academic Press, London, 2004), pp. 853–857

  2. F.F. Huang, Z.F. Sun, S.U. Emerson, R.H. Purcell, H.L. Shivaprasad, F.W. Pierson et al., J. Gen. Virol. 85, 1609–1618 (2004). doi:https://doi.org/10.1099/vir.0.79841-0

    Article  CAS  Google Scholar 

  3. L. Lu, C. Li, C.H. Hagedorn, Rev. Med. Virol. 16, 5–36 (2006). doi:https://doi.org/10.1002/rmv.482

    Article  CAS  Google Scholar 

  4. H. Okamoto, M. Takahashi, T. Nishizawa, K. Fukai, U. Muramatsu, A. Yoshikawa, Biochem. Biophys. Res. Commun. 289, 929–936 (2001). doi:https://doi.org/10.1006/bbrc.2001.6088

    Article  CAS  Google Scholar 

  5. X.J. Meng, R.H. Purcell, P.G. Halbur, J.R. Lehman, D.M. Webb, T.S. Tsareva et al., Proc. Natl. Acad. Sci. USA 94, 9860–9865 (1997). doi:https://doi.org/10.1073/pnas.94.18.9860

    Article  CAS  Google Scholar 

  6. M.S. Munne, S. Vladimirsky, L. Otegui, R. Castro, L. Brajterman, S. Soto et al., J. Med. Virol. 78, 1579–1583 (2006). doi:https://doi.org/10.1002/jmv.20741

    Article  Google Scholar 

  7. E.V. Koonin, A.E. Gorbalenya, M.A. Purdy, M.N. Rozanov, G.R. Reyes, D.W. Bradley, Proc. Natl. Acad. Sci. USA 89, 8259–8263 (1992). doi:https://doi.org/10.1073/pnas.89.17.8259

    Article  CAS  Google Scholar 

  8. J. Magden, N. Takeda, T. Li, P. Auvinen, T. Ahola, T. Miyamura et al., J. Virol. 75, 6249–6255 (2001). doi:https://doi.org/10.1128/JVI.75.14.6249-6255.2001

    Article  CAS  Google Scholar 

  9. M. Surjit, S. Jameel, S.K. Lal, J. Virol. 78, 320–328 (2004). doi:https://doi.org/10.1128/JVI.78.1.320-328.2004

    Article  CAS  Google Scholar 

  10. H. Korkaya, S. Jameel, D. Gupta, S. Tyagi, R. Kumar, M. Zafrullah et al., J. Biol. Chem. 276, 42389–42400 (2001). doi:https://doi.org/10.1074/jbc.M101546200

    Article  CAS  Google Scholar 

  11. W.H. van der Poel, F. Verschoor, R. van der Heide, M.I. Herrera, A. Vivo, M. Kooreman et al., Emerg. Infect. Dis. 7, 970–976 (2001)

    Article  Google Scholar 

  12. M. Banks, R. Bendall, S. Grierson, G. Heath, J. Mitchell, H. Dalton, Emerg. Infect. Dis. 10, 953–955 (2004)

    Article  Google Scholar 

  13. S. Fernandez-Barredo, C. Galiana, A. Garcia, S. Vega, M.T. Gomez, M.T. Perez-Gracia, J. Vet. Diagn. Invest. 18, 462–465 (2006)

    Article  Google Scholar 

  14. B. Olsen, D. Axelsson-Olsson, A. Thelin, O. Weiland, Scand. J. Infect. Dis. 38, 55–58 (2006). doi:https://doi.org/10.1080/00365540500321470

    Article  Google Scholar 

  15. D. Leblanc, P. Ward, M.J. Gagné, E. Poitras, P. Müller, Y.L. Trottier et al., Int. J. Food Microbiol. 117, 160–166 (2007). doi:https://doi.org/10.1016/j.ijfoodmicro.2007.03.008

    Article  CAS  Google Scholar 

  16. S. Fernández-Barredo, C. Galiana, A. García, M.T. Gómez-Muñoz, S. Vega, M.A. Rodríguez-Iglesias et al., Can. J. Vet. Res. 71, 236–240 (2007)

    PubMed  PubMed Central  Google Scholar 

  17. M. Takahashi, T. Nishizawa, H. Miyajima, Y. Gotanda, T. Iita, F. Tsuda et al., J. Gen. Virol. 84, 851–862 (2003). doi:https://doi.org/10.1099/vir.0.18918-0

    Article  CAS  Google Scholar 

  18. S. Tei, N. Kitajima, K. Takahashi, S. Mishiro, Lancet 362, 371–373 (2003). doi:https://doi.org/10.1016/S0140-6736(03)14025-1

    Article  Google Scholar 

  19. T.C. Li, K. Chijiwa, N. Sera, T. Ishibashi, Y. Etoh, Y. Shinohara et al., Emerg. Infect. Dis. 11, 1958–1960 (2005)

    Article  Google Scholar 

  20. P. Gyarmati, N. Mohammed, H. Norder, J. Blomberg, S. Belák, F. Widén, J. Virol. Methods 146, 226–235 (2007). doi:https://doi.org/10.1016/j.jviromet.2007.07.014

    Article  CAS  Google Scholar 

  21. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994). doi:https://doi.org/10.1093/nar/22.22.4673

    Article  CAS  Google Scholar 

  22. P.H.A. Sneath, R.R. Sokal, Numerical Taxonomy (W.H. Freeman and Company, San Francisco, 1973), pp. 230–234

    Google Scholar 

  23. J. Inoue, M. Takahashi, K. Ito, T. Shimosegawa, H. Okamoto, J. Gen. Virol. 87, 2363–2369 (2006). doi:https://doi.org/10.1099/vir.0.81912-0

    Article  CAS  Google Scholar 

  24. H. Okamoto, Virus Res. 127, 216–222 (2007). doi:https://doi.org/10.1016/j.virusres.2007.02.002

    Article  CAS  Google Scholar 

  25. C. Renou, J.F. Cadranel, M. Bourlière, P. Halfon, D. Ouzan, H. Rifflet et al., Emerg. Infect. Dis. 13, 1094–1096 (2007)

    Article  Google Scholar 

  26. G. Deest, L. Zehner, E. Nicand, C. Gaudy-Graffin, A. Goudeau, Y. Bacq, Gastroenterol. Clin. Biol. 31, 1095–1097 (2007). doi:https://doi.org/10.1016/S0399-8320(07)78342-2

    Article  Google Scholar 

  27. M.T. Pérez-Gracia, M.L. Mateos, C. Galiana, S. Fernández-Barredo, A. García, M.T. Gómez et al., Am. J. Trop. Med. Hyg. 77, 893–896 (2007)

    Article  Google Scholar 

  28. P. Shukla, U.K. Chauhan, S. Naik, D. Anderson, R. Aggarwal, J. Viral Hepat. 14, 295–297 (2007). doi:https://doi.org/10.1111/j.1365-2893.2006.00815.x

    Article  Google Scholar 

  29. M. Herremans, H. Vennema, J. Bakker, B. van der Veer, E. Duizer, C.A. Benne, K. Waar, B. Hendrixks, P. Schneeberger, G. Blaauw, M. Kooiman, M.P. Koopmans, J. Viral Hepat. 14, 140–146 (2007). doi:https://doi.org/10.1111/j.1365-2893.2006.00786.x

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the European Union project “Pathogen Combat”, contract number FOOD-CT-2005-007081 and European Union Network of Excellence “Network for the prevention and control of Zoonoses (Med-Vet-Net)”, contract number 506122. Support was also received from the “Ivar och Elsa Sandberg’s foundation”, Sweden.

Author information

Authors and Affiliations

  1. Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 751 89, Uppsala, Sweden

    Hongyan Xia, Lihong Liu, Anna-Malin Linde & Sándor Belák

  2. Joint R&D Division in Virology, The National Veterinary Institute & The Swedish University of Agricultural Sciences, 751 89, Uppsala, Sweden

    Hongyan Xia, Lihong Liu, Anna-Malin Linde, Sándor Belák & Frederik Widén

  3. Virological Department, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden

    Heléne Norder

Authors
  1. Hongyan Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lihong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anna-Malin Linde
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sándor Belák
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Heléne Norder
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Frederik Widén
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Frederik Widén.

Additional information

H. Xia and L. Liu have contributed equally to this paper.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xia, H., Liu, L., Linde, AM. et al. Molecular characterization and phylogenetic analysis of the complete genome of a hepatitis E virus from European swine. Virus Genes 37, 39–48 (2008). https://doi.org/10.1007/s11262-008-0246-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-008-0246-9

Keywords

Search

Navigation