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A non-radioactive PCR-SSCP analysis allows to distinguish between HPV 16 European and Asian-American variants in squamous cell carcinomas of the uterine cervix in Colombia

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Abstract

Human Papillomavirus type 16 (HPV 16) DNA is regularly found in around 50% of all cervical carcinomas. Variants of this type have been found associated with different risks for cervical cancer development. Presence of HPV 16 variants in Colombia has not been previously reported. The aims of this study were to assess the feasibility of non-radioactive PCR-SSCP (polymerase chain reaction single-strand conformation polymorphism) analysis for determination of variability of ORF of E6, variability in the enhancer sequence of the LCR, and for establishment of the distribution of HPV 16 variants in invasive squamous cell carcinoma of the uterine cervix in Colombian women. Biopsies from 59 patients at the Instituto Nacional de Cancerología (INC) in Bogotá (Colombia) were collected. HPV detection was performed using universal primers. HPV 16 variants were detected by non-radioactive single-stranded conformational polymorphism (SSCP) analysis and direct sequencing. HPV 16 was detected in 57.6% of the tumors. The European branch was identified in 88.2% of the samples with the E-G350 class being the most prevalent variant (41.1%). The Asian-American branch was identified in 8.8% of the samples. Within this group it was possible to distinguish between c and a classes. It was not possible to determine the branch in 2.9% of the cases. A nucleotide transition (G to A) at position 7521 was the most prevalent variation (80%) found in the enhancer sequence of the LCR region. Conclusion: A non-radioactive PCR-SSCP analysis allowed us to distinguish between European and Asian-American branches of HPV 16, and to distinguish among classes in squamous cell carcinomas of the uterine cervix in Colombia. This method is an excellent alternative that can be used as a screening tool for identification of HPV 16 variants.

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References

  1. D.M. Parkin, F. Bray, J. Ferlay, P. Pisani, Int. J. Cancer 94, 153–156 (2001). doi:https://doi.org/10.1002/ijc.1440

    Article  CAS  Google Scholar 

  2. M. Piñeros, J. Ferlay, R. Murillo, Instituto Nacional de Cancerología, Imprenta Nacional (2005)

  3. J.M. Walboomers, M.V. Jacobs, M.M. Manos, F.X. Bosch, J.A. Kummer, K.V. Shah, P.J. Snijders, J. Peto, C.J. Meijer, N. Munoz, J. Pathol. 189, 12–19 (1999). doi:https://doi.org/10.1002/(SICI) 1096-9896(199909) 189:1≤12::AID-PATH431≥3.0.CO;2-F

    Article  CAS  Google Scholar 

  4. G.M. Clifford, M.A. Goncalves, S. Franceschi, AIDS 20, 2337–2344 (2006). doi:https://doi.org/10.1097/01.aids.0000253361.63578.14

    Article  Google Scholar 

  5. N. Munoz, F. Mendez, H. Posso, M. Molano, A.J. van den Brule, M. Ronderos, C. Meijer, A. Munoz, J. Infect. Dis. 190, 2077–2087 (2004). doi:https://doi.org/10.1086/425907

    Article  Google Scholar 

  6. F.X. Bosch, M.M. Manos, N. Munoz, M. Sherman, A.M. Jansen, J. Peto, M.H. Schiffman, V. Moreno, R. Kurman, K.V. Shah, J. Natl. Cancer Inst. 87, 796–802 (1995). doi:https://doi.org/10.1093/jnci/87.11.796

    Article  CAS  Google Scholar 

  7. N. Munoz, F.X. Bosch, S. de Sanjose, R. Herrero, X. Castellsague, K.V. Shah, P.J. Snijders, C.J. Meijer, N. Engl. J. Med. 348, 518–527 (2003). doi:https://doi.org/10.1056/NEJMoa021641

    Article  Google Scholar 

  8. H.U. Bernard, I.E. Calleja-Macias, S.T. Dunn, Int. J. Cancer. 118, 1071–1076 (2006). doi:https://doi.org/10.1002/ijc.21655

    Article  CAS  Google Scholar 

  9. H.U. Bernard, J. Clin. Virol. 32(Suppl 1), S1–S6 (2005). doi:https://doi.org/10.1016/j.jcv.2004.10.021

    Article  Google Scholar 

  10. L. Ho, S.Y. Chan, R.D. Burk, B.C. Das, K. Fujinaga, J.P. Icenogle, T. Kahn, N. Kiviat, W. Lancaster, P. Mavromara-Nazos, J. Virol. 67, 6413–6423 (1993)

    CAS  PubMed  PubMed Central  Google Scholar 

  11. T. Yamada, M.M. Manos, J. Peto, C.E. Greer, N. Munoz, F.X. Bosch, C.M. Wheeler, J. Virol. 71, 2463–2472 (1997)

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Z. Chen, M. Terai, L. Fu, R. Herrero, R. DeSalle, R.D. Burk, J. Virol. 79, 7014–7023 (2005). doi:https://doi.org/10.1128/JVI.79.11.7014-7023.2005

    Article  CAS  Google Scholar 

  13. L. Sichero, H. Trottier, S. Ferreira, E. Duarte-Franco, E.L. Franco, L.L. Villa, J. Natl. Cancer. Inst. 99, 653–654 (2007). doi:https://doi.org/10.1093/jnci/djk136

    Article  Google Scholar 

  14. L. Sichero, S. Ferreira, H. Trottier, E. Duarte-Franco, A. Ferenczy, E.L. Franco, L.L. Villa, Int. J. Cancer. 120, 1763–1768 (2007). doi:https://doi.org/10.1002/ijc.22481

    Article  CAS  Google Scholar 

  15. M. Grodzki, G. Besson, C. Clavel, A. Arslan, S. Franceshi, P. Birembaut, M. Tommasino, I. Zehbe, Cancer Epidemiol. Biomarkers Prev. 15, 820–822 (2006). doi:https://doi.org/10.1158/1055-9965.EPI-05-0864

    Article  CAS  Google Scholar 

  16. J. Berumen, R.M. Ordonez, E. Lazcano, J. Salmeron, S.C. Galvan, R.A. Estrada, E. Yunes, A. Garcia-Carranca, G. Gonzalez-Lira, A. Madrigal-de la Campa, J. Natl. Cancer Inst. 93, 1325–1330 (2001). doi:https://doi.org/10.1093/jnci/93.17.1325

    Article  CAS  Google Scholar 

  17. I. Nindl, K. Rindfleisch, B. Lotz, A. Schneider, M. Durst, Int. J. Cancer 82, 203–207 (1999). doi:https://doi.org/10.1002/(SICI) 1097-0215(19990719) 82:2≤203::AID-IJC9≥3.0.CO;2-9

    Article  CAS  Google Scholar 

  18. M. Refugio Gonzalez-Losa, M. Laviada, M. Puerto-Solis, A. Garcia-Carranca, J. Clin. Virol. 29, 95–98 (2004). doi:https://doi.org/10.1016/S1386-6532(03) 00094-5

    Article  Google Scholar 

  19. I. Zehbe, J. Mytilineos, I. Wikstrom, R. Henriksen, L. Edler, M. Tommasino, Hum. Immunol. 64, 538–542 (2003). doi:https://doi.org/10.1016/S0198-8859(03) 00033-8

    Article  CAS  Google Scholar 

  20. C. Kammer, M. Tommasino, S. Syrjanen, H. Delius, U. Hebling, U. Warthorst, H. Pfister, I. Zehbe, Br. J. Cancer 86, 269–273 (2002). doi:https://doi.org/10.1038/sj.bjc.6600024

    Article  CAS  Google Scholar 

  21. M.J. O’Connor, W. Stunkel, H. Zimmermann, C.H. Koh, H.U. Bernard, J. Virol. 72, 10083–10092 (1998)

    PubMed  PubMed Central  Google Scholar 

  22. G. Veress, K. Szarka, X.P. Dong, L. Gergely, H. Pfister, J. Gen. Virol. 80, 1035–1043 (1999)

    Article  CAS  Google Scholar 

  23. T. Chong, W.K. Chan, H.U. Bernard, Nucleic Acids Res. 18, 465–470 (1990). doi:https://doi.org/10.1093/nar/18.3.465

    Article  CAS  Google Scholar 

  24. X.P. Dong, F. Stubenrauch, E. Beyer-Finkler, H. Pfister, Int. J. Cancer 58, 803–808 (1994). doi:https://doi.org/10.1002/ijc.2910580609

    Article  CAS  Google Scholar 

  25. K. Kurvinen, M. Yliskoski, S. Saarikoski, K. Syrjanen, S. Syrjanen, Eur. J. Cancer 36, 1402–1410 (2000). doi:https://doi.org/10.1016/S0959-8049(00) 00121-0

    Article  CAS  Google Scholar 

  26. J.R. Ellis, P.J. Keating, J. Baird, E.F. Hounsell, D.V. Renouf, M. Rowe, D. Hopkins, M.F. Duggan-Keen, J.S. Bartholomew, L.S. Young, Nat. Med. 1, 464–470 (1995). doi:https://doi.org/10.1038/nm0595-464

    Article  CAS  Google Scholar 

  27. S. Andersson, M. Alemi, E. Rylander, A. Strand, B. Larsson, J. Sallstrom, E. Wilander, Br. J. Cancer 83, 307–310 (2000). doi:https://doi.org/10.1054/bjoc.2000.1247

    Article  CAS  Google Scholar 

  28. O. Chakrabarti, K. Veeraraghavalu, V. Tergaonkar, Y. Liu, E.J. Androphy, M.A. Stanley, S. Krishna, J. Virol. 78, 5934–5945 (2004). doi:https://doi.org/10.1128/JVI.78.11.5934-5945.2004

    Article  CAS  Google Scholar 

  29. A. Giannoudis, C.S. Herrington, J. Pathol. 193, 295–302 (2001). doi:https://doi.org/10.1002/1096-9896(2000) 9999:9999≤::AID-PATH809≥3.0.CO;2-C

    Article  CAS  Google Scholar 

  30. P. Londesborough, L. Ho, G. Terry, J. Cuzick, C. Wheeler, A. Singer, Int. J. Cancer 69, 364–368 (1996). doi:https://doi.org/10.1002/(SICI) 1097-0215(19961021) 69:5≤364::AID-IJC2≥3.0.CO;2-3

    Article  CAS  Google Scholar 

  31. I. Zehbe, G. Voglino, H. Delius, E. Wilander, M. Tommasino, Lancet 352, 1441–1442 (1998). doi:https://doi.org/10.1016/S0140-6736(05) 61263-9

    Article  CAS  Google Scholar 

  32. M. Grodzki, G. Besson, C. Clavel, A. Arslan, S. Franceschi, P. Birembaut, M. Tommasino, I. Zehbe, Cancer Epidemiol. Biomarkers Prev. 15, 820–822 (2006). doi:https://doi.org/10.1158/1055-9965.EPI-05-0864

    Article  CAS  Google Scholar 

  33. R.K. Saiki, D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis, H.A. Erlich, Science 239, 487–491 (1988). doi:https://doi.org/10.1126/science.2448875

    Article  CAS  Google Scholar 

  34. A.M. Roda Husman, J.M. Walboomers, A.J. van den Brule, C.J. Meijer, P.J. Snijders, J. Gen. Virol. 76, 1057–1062 (1995)

    Article  Google Scholar 

  35. M. Schmidt, W. Kedzia, A. Gozdzicka-Jozefiak, J. Clin. Virol. 23, 65–77 (2001). doi:https://doi.org/10.1016/S1386-6532(01) 00189-5

    Article  CAS  Google Scholar 

  36. M. Alemi, S. Andersson, J. Sallstrom, E. Wilander, Diagn. Mol. Pathol. 8, 97–100 (1999). doi:https://doi.org/10.1097/00019606-199906000-00006

    Article  CAS  Google Scholar 

  37. K. Hayashi, D.W. Yandell, Hum. Mutat. 2, 338–346 (1993). doi:https://doi.org/10.1002/humu.1380020503

    Article  CAS  Google Scholar 

  38. I. Zehbe, J.F. Sallstrom, M. Evander, K. Edlund, E. Rylander, G. Wadell, E. Wilander, Diagn. Mol. Pathol. 5, 206–213 (1996). doi:https://doi.org/10.1097/00019606-199609000-00010

    Article  CAS  Google Scholar 

  39. J.D. Meissner, J. Gen. Virol. 80, 1725–1733 (1999)

    Article  CAS  Google Scholar 

  40. G. Myers, H.U. Bernard, H. Delius, M. Favre, J. Icenogle, M. Van Rast, C. Wheeler (eds.), Human Papillomaviruses 1996: A Compilation and Analysis of Nucleic Acid and Amino Acid Sequences (Los Alamos National Laboratory, Los Alamos, NM, USA, 1996)

  41. T. Yamada, C.M. Wheeler, A.L. Halpern, A.C. Stewart, A. Hildesheim, S.A. Jenison, J. Virol. 69, 7743–7753 (1995)

    CAS  PubMed  PubMed Central  Google Scholar 

  42. H. Lichtig, M. Algrisi, L.E. Botzer, T. Abadi, Y. Verbitzky, A. Jackman, M. Tommasino, I. Zehbe, L. Sherman, Virology 350, 216–227 (2006). doi:https://doi.org/10.1016/j.virol.2006.01.038

    Article  CAS  Google Scholar 

  43. J.S. Park, E.S. Hwang, C.J. Lee, C.J. Kim, J.G. Rha, S.J. Kim, S.E. Namkoong, S.J. Um, Gynecol. Oncol. 74, 23–29 (1999). doi:https://doi.org/10.1006/gyno.1999.5399

    Article  CAS  Google Scholar 

  44. T.R. Alencar, D.M. Cerqueira, M.R. da Cruz, P.S. Wyant, E.D. Ramalho, C.R. Martins, Vírus Genes 35, 1–4 (2007). doi:https://doi.org/10.1007/s11262-006-0040-5

    CAS  PubMed  Google Scholar 

  45. M. O’Connor, S.Y. Chan, H.U. Bernard, in Human Papillomaviruses 1995 Compendium, ed. by G. Myers, H.U. Bernard, H. Delius, C. Baker, J. Icenogle, A. Halpern, C. Wheeler (Los Alamos National Laboratory, Los Alamos, NM, USA, 1995), pp. 21–40

  46. M.J. O’Connor, S.H. Tan, C.H. Tan, H.U. Bernard, J. Virol. 70, 6529–6539 (1996)

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

We thank Bernardo Avellaneda for his help in the preparation of section biopsies. We also acknowledge to Marcela Lizano for critical reading of the manuscript. This work received financial support from the Instituto Nacional de Cancerología (Colombia) through the investment project “Use of Cold Probes in the Study of the Relation between HPV and Cancer of the Uterine Cervix” (Code 4103038-1).

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Authors and Affiliations

  1. Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Calle 1a. No. 9-85, Bogota, D. C., Colombia

    Pablo Moreno-Acosta, Mónica Molano, Antonio Huertas & María Mercedes Bravo

  2. Laboratorio de Hormonas, Departamento de Química, Universidad Nacional de Colombia, Carrera 30 Calle 45, Bogota, D. C., Colombia

    Myriam Sánchez de Gómez

  3. Patología, Instituto Nacional de Cancerología, Calle 1a. No 9-85, Bogota, D. C., Colombia

    Alfredo Romero

  4. Clínica de Ginecología, Instituto Nacional de Cancerología, Calle 1a. No 9-85, Bogota, D. C., Colombia

    Mauricio González

  5. Unidad de Investigación Biomédica en Cáncer, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México – Instituto Nacional de Cancerología, Secretaría de Salud, Mexico, D. F., Mexico

    Alejandro García-Carrancá

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  1. Pablo Moreno-Acosta
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Correspondence to Pablo Moreno-Acosta.

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Moreno-Acosta, P., Molano, M., Huertas, A. et al. A non-radioactive PCR-SSCP analysis allows to distinguish between HPV 16 European and Asian-American variants in squamous cell carcinomas of the uterine cervix in Colombia. Virus Genes 37, 22–30 (2008). https://doi.org/10.1007/s11262-008-0239-8

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