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Complete nucleotide sequence of a new strain of Tobacco necrosis virus A infecting soybean in China and infectivity of its full-length cDNA clone

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Abstract

The complete nucleotide sequence of a virus isolated from soybean (Glycine max (L.) Merr.) in China, previously identified as a new strain of Tobacco necrosis virus A (TNV-A) based on its biological, serological properties, and coat protein (CP) sequence and named as TNV-AC, was determined and compared with that of TNV-A and other closely related Necroviruses and Carmoviruses. The viral RNA genome consists of 3,682 nucleotides and contains five open reading frames (ORFs). TNV-AC showed 86.4% overall nucleotide sequence identity to TNV-A. The CP and putative RNA-dependent RNA polymerase (RdRp) showed 88.8 and 95.9% amino acid identity, respectively, to that of TNV-A. The greatest difference between TNV-AC and TNV-A was in the 3′ terminal region: the p7K ORF region present in TNV-A was absent in TNV-AC. Phylogenetic analysis of RdRp, CP, and small ORF regions of Necroviruses confirmed TNV-AC as a new strain of TNV-A. A full-length cDNA clone of TNV-AC was constructed and used as template for run-off transcription based on the obtained sequence. The results indicate that the in vitro-synthesized viral RNA faithfully represented the biological activity of wild-type TNV-AC.

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Acknowledgments

We would like to thank professors Qi Cao and Zuqin Pu (college of plant protein, Nanjing Agricultural University) for providing the TNV-AC, Dr. Renate Koenig (Biologische Bundesanstalt für Land- und Forstwirtschaft, Germany) for providing antiserum against TNV willow isolate, Dr. Taiowa Montgomery (Center for Genome Research and Biocomputing, Oregon State University) for improving the English. This work was supported by National Nature Science Foundation of China (30325001 and 30470077).

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Author notes

  1. Dehui Xi

    Present address: College of Life Sciences, Sichuan University, Chengdu, 610064, China

Authors and Affiliations

  1. State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100094, P.R. China

    Dehui Xi, Jiang Li, Chenggui Han, Dawei Li & Jialin Yu

  2. Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Xueping Zhou

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  1. Dehui Xi
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Corresponding author

Correspondence to Dawei Li.

Additional information

The GenBank Accession No. of the sequence reported in this article is AY546104.

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Xi, D., Li, J., Han, C. et al. Complete nucleotide sequence of a new strain of Tobacco necrosis virus A infecting soybean in China and infectivity of its full-length cDNA clone. Virus Genes 36, 259–266 (2008). https://doi.org/10.1007/s11262-007-0185-x

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  • DOI: https://doi.org/10.1007/s11262-007-0185-x

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