Abstract
National Institute of Virology, India has instituted molecular surveillance of measles strains in the country. In phased manner, three major cities Pune, Chennai and Bangalore were covered. Throat swab and urine from suspected measles cases from Chennai and Pune and freshly frozen brain tissues, CSF from suspected SSPE case from Bangalore were subjected to RNA extraction and Measles N&H gene RT-PCR as per WHO standard protocols. PCR positive products were sequenced. Sequence alignment and phylogenetic analysis was carried out using WHO standard reference sequences. Virus isolation was attempted using B95a cell line. Measles genotype D7 was detected from two classical measles cases (Chennai and Pune) and in a fulminant SSPE case (Bangalore). This is the first detection of measles genotype D7 from India.
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Acknowledgements
Authors would like to thank Dr. A. C. Mishra, Director, National Institute of Virology, Pune for permitting to publish this study. Dr. Naseem Shaikh and Mr. Laxman Hungund are thanked for performance of serology tests. Authors would like to thank World Health Organization, Initiative on Vaccine Research (WHO-IVR) for project funding. Contributions of the staff of all the three institutions are duly acknowledged. Financial Support: World Health Organization, Initiative on Vaccine Research (WHO-IVR), Geneva provided partial funds under the project “ Genetic characterization of measles strains circulating in India” with Dr. Niteen Wairagkar as Principal Investigator.
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Vaidya, S.R., Wairagkar, N.S., Raja, D. et al. First detection of measles genotype D7 from India. Virus Genes 36, 31–34 (2008). https://doi.org/10.1007/s11262-007-0172-2
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DOI: https://doi.org/10.1007/s11262-007-0172-2