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A divergent isolate of Tomato yellow leaf curl virus from Oman with an associated DNAβ satellite: an evolutionary link between Asian and the Middle Eastern virus–satellite complexes

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Abstract

Tomato is cultivated in the coastal region of Al-Batinah, in the Sultanate of Oman, during the winter season, to meet the high demand for fresh produce in the domestic market. In order to identify the causal agent of a widespread disease associated with infestations of the whitefly Bemisia tabaci (Genn.) leaves were collected from tomato plants showing symptoms characteristic of the disease in Al-Batinah during 2004 and 2005. Total nucleic acids were isolated from the tomato leaves and used as the template for Φ29 DNA polymerase amplification of begomoviral circular DNA. Putative full unit length begomoviral DNA multimers were digested with Nco I and cloned into the plasmid vector pGEM7Zf+. The complete nucleotide (nt) sequence was determined as 2,765 bases, indicative of a monopartite begomoviral genome. A comparison of the genome sequence for the seven field isolates examined, indicated that they shared 99% nt identity. The virus from Oman was most closely related to TYLCV-IR at 91% nt identity, a monopartite begomoviral species described previously from Iran. Based on the guidelines of the ICTV the Oman isolate has been designated TYLCV-Om and is considered an isolate of TYLCV-IR. A satellite DNA (satDNA β), was amplified by polymerase chain reaction using degenerate primers and cloned, and the DNA sequence was determined. Analysis of the complete nt sequence of 1,371 bases indicated that the satDNA shared 88.5% similarity with its closest relatives, which are DNAβ molecules from tomato in Pakistan. This is the first report of a satDNA β associated with the TYLCV species. The TYLCV-Om and associated satDNA, thus represent a begomovirus-complex at the Asian-Middle East crossroads that quiet uniquely share geographical and genetic hallmarks of both.

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References

  1. H. Czosnek, H. Laterrot, Arch. Virol. 142, 1391–1406 (1977)

    Article  Google Scholar 

  2. J.K. Brown, Virus Res. 71, 233–260 (2000)

    Article  PubMed  CAS  Google Scholar 

  3. C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (eds), Virus Taxonomy: Eight Report of the International Committee on Taxonomy of Viruses (Elsevier Academic Press, UK, 2005)

  4. C.M. Fauquet, S. Sawyer, A.M. Idris, J.K. Brown, Phytopathology 95, 549–555 (2005)

    Article  CAS  PubMed  Google Scholar 

  5. A. Kheyr-Pour, M. Bendahmane, V. Matzeit, G.P. Accotto, S. Crespi, B. Gronenborn, Nucleic Acids Res. 19, 6763–6769 (1991)

    Article  PubMed  CAS  Google Scholar 

  6. N. Navot, E. Pichersky, M. Zeidan, D. Zamir, H. Czosnek, Virology 185, 151–161 (1991)

    Article  PubMed  CAS  Google Scholar 

  7. I.B. Dry, J.E. Ridgen, L.R. Krake, P.M. Mullineaux, M.A. Rezaian, J. Gen. Virol. 74, 147–151 (1993)

    PubMed  CAS  Google Scholar 

  8. A.M. Idris, R.W. Briddon, S.E. Bull, J.K. Brown, Virus Res. 109, 19–32 (2005)

    Article  PubMed  CAS  Google Scholar 

  9. M.R. Hajimorad, A. Kheyr-Pour, V. Alavi, A. Ahoonmanesh, M. Bahar, M.A. Rezaian, B. Gronenborn, Plant Pathol. 45, 418–425 (1996)

    Article  Google Scholar 

  10. S. Cohen, I. Harpez, Entomol. Exp. Appl. 7, 155–166 (1964)

    Article  Google Scholar 

  11. S. Cohen, Y. Antigus, Adv. Dis. Vector Res. 10, 259–288 (1994)

    Google Scholar 

  12. A.M. Idris, J.K. Brown, Phytopathology 94, 1068–1074 (2004)

    Article  CAS  PubMed  Google Scholar 

  13. J.J. Doyle, J.L. Doyle, Phytochem. Bull. 19, 11–15 (1987)

    Google Scholar 

  14. A.M. Idris, J.K. Brown, Phytopathology 88, 648–657 (1998)

    Article  CAS  PubMed  Google Scholar 

  15. A.K. Inoue-Nagata, L.C. Albuquerque, W.B. Rocha, T. Nagata, J. Virol. Methods 116, 209–211 (2004)

    Google Scholar 

  16. D.L. Swofford, PAUP* Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10. Sinauer Associates, Sunderland, MA, (2002)

  17. S. Sawyer, Mol. Biol. Evol. 6, 526–538 (1989)

    PubMed  CAS  Google Scholar 

  18. M. Padidam, S. Sawyer, C.M. Fauquet, Virology 265, 218–225 (1999)

    Article  PubMed  CAS  Google Scholar 

  19. G.F. Weiller, Mol. Biol. Evol. 15, 326–335 (1998)

    PubMed  CAS  Google Scholar 

  20. A.M. Idris, J.K. Brown, Virus Genes 24, 249–256 (2002)

    Article  PubMed  CAS  Google Scholar 

  21. K. Bananej, A. Kheyr-Pour, G. Hosseini Salekdeh, A. Ahoonmanesh, Arch. Virol. 149, 1435–1443 (2004)

    Article  PubMed  CAS  Google Scholar 

  22. X. Cui, T. Xiaorong, Y. Xie, C.M. Fauquet, X. Zhou, J. Virol. 78, 13966–13974 (2004)

    Article  PubMed  CAS  Google Scholar 

  23. K. Saunders, A. Norman, S. Gucciardo, J. Stanely, Virology 324, 37–47 (2004)

    Article  PubMed  CAS  Google Scholar 

  24. J. Laufs, W. Traut, F. Heyraud, V. Matzeit, S.G. Rogers, J. Schell, B. Gronenborn, Proc. Natl. Acad. Sci., USA 92, 3879–3883 (1995)

    Article  PubMed  CAS  Google Scholar 

  25. R.W. Briddon, E.B. Simon, I. Amin, A.M. Idris, S. Mansoor, I.D. Bedford, P. Dhawan, N. Rishi, S.S. Siwatch, A.M. Abdel-Salam, J.K. Brown, Y. Zafar, P.G. Markham, Virology 312, 106–121 (2003)

    Article  PubMed  CAS  Google Scholar 

  26. M. Padidam, R.N. Beachy, C.M. Fauquet, J. Gen. Virology 76, 249–263 (1995)

    Article  CAS  Google Scholar 

  27. E.P. Rybicki, Arch. Virol. 139, 49–77 (1994)

    Article  PubMed  CAS  Google Scholar 

  28. E.P. Rybicki, R.W. Briddon, J.K. Brown, C.M. Fauquet, D.P. Maxwell, B.D. Harrison, P.G. Markham, D.M. Bisaro, D. Robinson, J. Stanely, in Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses, ed. by M.H.V. Van Regenmortal, C.M. Fauquet, D.H.L. Bishop, E.B. Carstens, M.K. Estes, S.M. Lemon, J. Maniloff, M.A. Mayo, D.J. McGeoch, C.R. Pringle, R.B. Wicker (Academic Press, New York, 2000), pp. 285–297

  29. S. Attathom, P. Chiemsombat, W. Kositratana, N. Sae-Ung, Kasetsart. J. Soc. Sci. 28, 632–639 (1994)

    CAS  Google Scholar 

  30. A.M. Idris, J.K. Brown, Arch. Virol. 150, 1003–1012 (2005)

    Article  PubMed  CAS  Google Scholar 

  31. J. Navas-Castillo, S. Sanchez-Campos, E. Noris, D. Louro, G.P. Accotto, E. Morionesm, J. Gen. Virol. 81, 2797–2801 (2000)

    PubMed  CAS  Google Scholar 

  32. A.I. Sanz, A. Fraile, F. Garcia-Arenal, X. Zhou, D.J. Robinson, S. Khalid, T. Butt, B.D. Harrison, J. Gen. Virol. 81, 1839–1849 (2000)

    PubMed  CAS  Google Scholar 

  33. O. Chatchawankanphanich, D.P. Maxwell, Phytopathology 92, 637–645 (2002)

    Article  PubMed  Google Scholar 

  34. M. Zeidan, S.K. Green, D.P. Maxwell, M.K. Nakhla, H. Czosnek, Trop. Agric. Res. Exten. 1, 107–115 (1998)

    Google Scholar 

  35. I.B. Dry, L.R. Krake, J.E. Rigden, M.A. Rezaian, Proc. Natl. Acad. Sci. USA 94, 7088–7093 (1997)

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Crop Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos University, P.O. Box 34, Al-Khod 123, Muscat, Sultanate of Oman

    Akhtar Jamal Khan, Nadiya Abubaker Al-Saady, Madleen Said Al-Mahruki & Ali Masoud Al-Subhi

  2. Department of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA

    Ali M. Idris & J. K. Brown

Authors
  1. Akhtar Jamal Khan
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  2. Ali M. Idris
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  3. Nadiya Abubaker Al-Saady
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  4. Madleen Said Al-Mahruki
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  5. Ali Masoud Al-Subhi
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  6. J. K. Brown
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Correspondence to Ali M. Idris.

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Khan, A.J., Idris, A.M., Al-Saady, N.A. et al. A divergent isolate of Tomato yellow leaf curl virus from Oman with an associated DNAβ satellite: an evolutionary link between Asian and the Middle Eastern virus–satellite complexes. Virus Genes 36, 169–176 (2008). https://doi.org/10.1007/s11262-007-0163-3

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  • DOI: https://doi.org/10.1007/s11262-007-0163-3

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