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Molecular characterization of the largest and smallest genome segments, S1 and S12, of Rice gall dwarf virus

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Abstract

The nucleotide sequences of segments S1 and S12 of a Chinese isolate of Rice gall dwarf virus (RGDV) were determined. This provides the first complete sequences of these segments. The complete sequence of S1, the largest genome segment of RGDV, was 4,505 nucleotides in length and was predicted to encode a large protein of 1,458 amino acids with a calculated molecular mass of nearly 166.2 kDa. The protein was related to that encoded by S1 of Rice dwarf virus (RDV; 50% identity and 67% similarity) and (to a lesser extent) to some large proteins of other reoviruses. It appears to be an RNA-dependent RNA polymerase (RdRp) and is probably present in particles as a minor core protein. S12, the smallest genome segment of RGDV, was 853 nucleotides in length, encoding a single major protein of 206 amino acids with a calculated molecular mass of nearly 23.6 kDa. This protein, though a little larger than those of RDV S11 and Wound tumor virus (WTV) S12 in size, showed some similarity to them, especially in the conserved N-terminal region and may have RNA-binding properties. Despite having a common host plant, RDV and RGDV were not more closely related to one another than either of them was to WTV. Phylogenetic analysis of the RdRp showed that members of the genus Phytoreovirus were more closely related to those of the genus Rotavirus than to any other genus within the family Reoviridae.

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Acknowledgments

This work was funded by the Zhejiang Provincial Foundation for Natural Science (Z305165) and two special grants from the Zhejiang Provincial Department of Science and Technology (2005E10010 and 2005C22027). We thank Mrs Liu Guimei and Mr Chen Hui, Station of Plant Protection and Quarantine, Agricultural Bureau of Chenxi county, Guangxi Province, for providing virus-infected rice plants. Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Plant Virology, Key Laboratory of Animal and Plant Virology, Ministry of Agriculture, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R. China

    Heng-Mu Zhang, Jian Yang, Xing Xin & Jian-Ping Chen

  2. Plant–Pathogen Interactions Division, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

    Michael J. Adams

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  1. Heng-Mu Zhang
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  2. Jian Yang
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  3. Xing Xin
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  4. Jian-Ping Chen
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  5. Michael J. Adams
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Correspondence to Heng-Mu Zhang.

Additional information

The nucleotide sequence data reported in this article have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers DQ333946 and DQ494209.

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Zhang, HM., Yang, J., Xin, X. et al. Molecular characterization of the largest and smallest genome segments, S1 and S12, of Rice gall dwarf virus . Virus Genes 35, 815–823 (2007). https://doi.org/10.1007/s11262-007-0142-8

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  • DOI: https://doi.org/10.1007/s11262-007-0142-8

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