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Suppression of porcine reproductive and respiratory syndrome virus replication in MARC-145 cells by shRNA targeting ORF1 region

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Abstract

Porcine reproductive and respiratory syndrome (PRRS) is an economically important disease in swine producing area. The current vaccine strategies cannot provide complete protection against PRRSV. The objective of this study was to determine if specific short-hairpin RNA (shRNA) directed against different genomic regions of ORF1b of PRRSV could be utilized to inhibit virus replication in MARC-145 cells. Two shRNA expression vectors targeting ORF1b gene of PRRSV were constructed and delivered into MARC-145 cells, and then infected with PRRSV. The results showed that PRRSV-specific cytopathic effect (CPE) could be inhibited in the cells transfected with pSUPER-P2 and pSUPER-P3, and the virus titers in the cells transfected with pSUPER-P2 and pSUPER-P3 were lower than those control cells by approximately 100 fold. Moreover, the expression of ORF1 of PRRSV in the cells was reduced both at RNA and protein levels comparing to the controls. It indicated that vector-based shRNA targeting ORF1 region could effectively inhibit PRRSV replication in MARC-145 cells.

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Acknowledgments

This work was supported by Program for New Century Excellent Talents in University (NCET-04-0502) and grants form the National Natural Science Foundation (30471288), “863”National High-tech Research and Development Program (2006AA10A203), and Foundation for PhD students training program, Ministry of Education (20060307007).

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Authors and Affiliations

  1. Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, 210095, P.R. China

    Guanming Li, Juan Huang, Ping Jiang, Yufeng Li, Wenming Jiang & Xianwei Wang

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  1. Guanming Li
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  2. Juan Huang
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  3. Ping Jiang
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  4. Yufeng Li
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  5. Wenming Jiang
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Correspondence to Ping Jiang.

Additional information

Guanming Li and Juan Huang contributed equally to this work.

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Li, G., Huang, J., Jiang, P. et al. Suppression of porcine reproductive and respiratory syndrome virus replication in MARC-145 cells by shRNA targeting ORF1 region. Virus Genes 35, 673–679 (2007). https://doi.org/10.1007/s11262-007-0134-8

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  • DOI: https://doi.org/10.1007/s11262-007-0134-8

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