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Marek’s disease virus unique genes pp38 and pp24 are essential for transactivating the bi-directional promoters for the 1.8 kb mRNA transcripts

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Abstract

The pp38 and pp24 genes of Marek’s diseases virus (MDV) share the same promoter, which controls the transcription of pp38 or pp24 and a 1.8-kb mRNA bi-directionally. To understand the trans-activating activity of pp38 and pp24 on the bi-directional promoter, both genes were cloned into pcDNA-3 or pBudCE4.1 vectors either singly or in combination. These plasmids were expressed in transfected chicken embryonic fibroblast (CEF) cells. Chloramphenicol acetyltransferase (CAT) activity expressed under the control of the promoter in CEF co-transfected with pP(1.8 kb)-CAT and pBud-pp38-pp24 was significantly higher than that following transfection with only pBud-pp38 or pBud-pp24. This indicates the combination of pp24 and pp38 together are essential for the activation of the promoter. In DNA mobility shift assays, the promoter binds to pp38 and pp24 together, but not to pp38 or pp24 alone. By competitive inhibition tests with a set of DNA fragments from the promoter region, the sequence 5′-CTGCTCATTT-3′ was identified as the core sequence for binding by pp38-pp24 in up-regulation of the bi-directional promoter activity.

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References

  1. S.J. Baigent, L.J. Ross, T.F. Davison, J. Gen. Virol. 79(Pt 11), 2795–2802 (1998)

    Article  CAS  PubMed  Google Scholar 

  2. G. Bradley, M. Hayashi, G. Lancz, A. Tanaka, M. Nonoyama, J. Virol. 63, 2534–2542 (1989)

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Z.Z. Cui, D. Yan, L.F. Lee, Virus Genes 3, 309–322 (1990)

    Article  CAS  PubMed  Google Scholar 

  4. Z.Z. Cui, L.F. Lee, J.L. Liu, H.J. Kung, J. Virol. 65, 6509–6515 (1991)

    CAS  PubMed  PubMed Central  Google Scholar 

  5. K. Ikuta, K. Nakajima, M. Naito, S.H. Ann, S. Ueda, S. Kato, K. Hirai, Int. J. Cancer 35, 257–264 (1985)

    Article  CAS  PubMed  Google Scholar 

  6. D. Jones, L. Lee, J.L. Liu, H.J. Kung, J.K. Tillotson, Proc. Natl. Acad. Sci. USA 89, 4042–4046 (1992)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Q. Xie, A.S. Anderson, R.W. Morgan, J. Virol. 70, 1125–1131 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  8. J.L. Liu, Y. Ye, L.F. Lee, H.J. Kung, J. Virol. 72, 388–395 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  9. J.L. Liu, S.F. Lin, L. Xia, P. Brunovskis, D. Li, I. Davidson, L.F. Lee, H.J. Kung, Acta Virol. 43, 94–101 (1999)

    CAS  PubMed  Google Scholar 

  10. J.L. Liu, H.J. Kung, Virus Genes 21, 51–64 (2000)

    Article  PubMed  Google Scholar 

  11. B. Lupiani, L.F. Lee, X. Cui, I. Gimeno, A. Anderson, R.W. Morgan, R.F. Silva, R.L. Witter, H.J. Kung, S.M. Reddy, Proc. Natl. Acad. Sci. USA 101, 11815–11820 (2004)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. X.B. Chen, P.J. Sondermeijer, L.F. Velicer, J. Virol. 66, 85–94 (1992)

    CAS  PubMed  PubMed Central  Google Scholar 

  13. D. Endoh, M. Niikura, K. Hirai, N. Inagaki, M. Hayashi, K. Nakajima, F. Sato, J. Vet Med. Sci. 56, 823–826 (1994)

    Article  CAS  PubMed  Google Scholar 

  14. W.D. Pratt, J. Cantello, R.W. Morgan, K.A. Schat, Virology 201, 132–136 (1994)

    Article  CAS  PubMed  Google Scholar 

  15. K. Nakajima, K. Ikuta, M. Naito, S. Ueda, S. Kato, K. Hirai, J. Gen. Virol. 68(Pt 5), 1379–1389 (1987)

    Article  CAS  PubMed  Google Scholar 

  16. M.S. Parcells, R.L. Dienglewicz, A.S. Anderson, R.W. Morgan, J. Virol. 73, 1362–1373 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  17. S.M. Reddy, B. Lupiani, I.M. Gimeno, R.F. Silva, L.F. Lee, R.L. Witter, Proc. Natl. Acad. Sci. USA 99, 7054–7059 (2002)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Z.Z. Cui, A. Qin, in Current Research on Marek’s Disease, ed. By R.F. Silva, H.H. Cheng, P.M. Coussens, L.F. Lee, L.F. Velicer (American Association of Avian Pathologiests, Kennett Square, PA, 1996), pp. 278–283

  19. W.D. Pratt, R. Morgan, K.A. Schat, Vet. Microbiol. 33, 93–99 (1992)

    Article  CAS  PubMed  Google Scholar 

  20. Q. Peng, M. Zeng, Z.A. Bhuiyan, E. Ubukata, A. Tanaka, M. Nonoyama, Y. Shirazi, Virology 213, 590–599 (1995)

    Article  CAS  PubMed  Google Scholar 

  21. R.L. Witter, R.F. Silva, L.F. Lee, Avian Dis. 31, 829–840 (1987)

    Article  CAS  PubMed  Google Scholar 

  22. G.S. Zhu, T. Ojima, T. Hironaka, T. Ihara, N. Mizukoshi, A. Kato, S. Ueda, K. Hirai, Avian Dis. 36, 637–645 (1992)

    Article  CAS  PubMed  Google Scholar 

  23. R.F. Silva, S.M. Reddy,B. Lupiani, J. Virol. 78, 733–740 (2004)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. H. Shigekane, Y. Kawaguchi, M. Shirakata, M. Sakaguchi, K. Hirai, Arch. Virol. 144, 1893–1907 (1999)

    Article  CAS  PubMed  Google Scholar 

  25. R.F. Silva, L.F. Lee, Virology 136, 307–320 (1984)

    Article  CAS  PubMed  Google Scholar 

  26. G.D. Smith, V. Zelnik, L.J. Ross, Virology 207, 205–216 (1995)

    Article  CAS  PubMed  Google Scholar 

  27. H.S. Camp, P.M. Coussens, R.F. Silva, J. Virol. 65, 6320–6324 (1991)

    CAS  PubMed  PubMed Central  Google Scholar 

  28. A. Katsumata, A. Iwata, S. Ueda, J. Gen. Virol. 79(Pt 12), 3015–3018 (1998)

    Article  CAS  PubMed  Google Scholar 

  29. G.S. Zhu, A. Iwata, M. Gong, S. Ueda, K. Hirai, Virology 200, 816–820 (1994)

    Article  CAS  PubMed  Google Scholar 

  30. J.B. Ding, Z.Z. Cui, L.F. Lee, X. Cui, S.J. Reddy, Virus Genes 32, 193–201 (2006)

    Article  CAS  PubMed  Google Scholar 

  31. J.B. Ding, Z.Z. Cui, S.J. Jiang, S.J. Reddy, Sci. Chan. Ser. C Life Sci. 49, 153–162 (2006)

    Google Scholar 

  32. L.F. Lee, X. Liu, R.L. Witter, J. Immunol. 130, 1003–1006 (1983)

    CAS  PubMed  Google Scholar 

  33. T.F. Wu, H.H. Chen, H. Wu, Virus Genes 23, 227–239 (2001)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grants 30300450 and 30070544 from the National Natural Science Foundation of China. We wish to thank Dr. Larry Bacon for critical review and editorial assistance of this manuscript.

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Authors and Affiliations

  1. Animal Science and Technology College, Shandong Agricultural University, Taian, Shandong, 271018, China

    Jiabo Ding & Zhizhong Cui

  2. China Institute of Veterinary Drug Control, Beijing, 100081, China

    Jiabo Ding

  3. U. S. Department of Agriculture, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI, 48823, USA

    Lucy F. Lee

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  1. Jiabo Ding
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  2. Zhizhong Cui
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  3. Lucy F. Lee
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Correspondence to Zhizhong Cui.

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Ding, J., Cui, Z. & Lee, L.F. Marek’s disease virus unique genes pp38 and pp24 are essential for transactivating the bi-directional promoters for the 1.8 kb mRNA transcripts. Virus Genes 35, 643–650 (2007). https://doi.org/10.1007/s11262-007-0129-5

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  • DOI: https://doi.org/10.1007/s11262-007-0129-5

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