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Complete nucleotide sequence of a Chinese serotype Asia1 vaccine strain of foot-and-mouth disease virus

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Abstract

The full-length nucleotide sequence of the Chinese vaccine strain Asia1/YNBS/58 of foot-and-mouth disease virus (FMDV) was determined. The results showed that the complete genome of YNBS/58 is 8,164 nucleotides (nt) in length including a 1,061-nt 5′ untranslated region (UTR), a 6,990-nt open reading frame (ORF), and a 113-nt 3′ UTR. Genome sequences of Asia1/YNBS/58 and other known FMDV strains were compared. The homology analysis indicated that non-structural proteins are more conserved than structural proteins in FMDV and that the 5′ UTR is more conserved than the 3′ UTR. Phylogenetic analysis revealed that Asia1/YNBS/58 is clustered in the Asia1 serotype and is linked to three other isolates, Asia1/IND/63/72, Asia1/3kimron/63, and Asia1/2ISRL/63, suggesting that they have a close genetic relationship. The VP1-, VP2-, and VP3-based phylogenetic trees divided into distinct clusters according to the different serotypes, while other gene-based phylogenetic trees exhibited some degree of intercrossing among serotypes. According to the nucleotide similarities between Asia1/YNBS/58 and two recent Chinese Asia1 isolates, Asia1/HKN/05, and Asia1/JS/05, each forms a distinct genotype. This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.

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Acknowledgments

This work was supported by the Chinese National Key Basic Research Program (973 program) Grant (No. 2005CB523201), the Chinese National Key Technology R&D Program Grant (No.2006BAD06A03), and the Chinese High Technology Research and Development program (863 program) Grant (No.2006AA10A204). We thank Dr. Yong XIE from Hong Kong University of Science and Technology for carefully reading the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Animal Virology of the Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China

    Junzheng Du, Huiyun Chang, Guozheng Cong, Junjun Shao, Tong Lin, Youjun Shang, Zaixin Liu, Xiangtao Liu, Xuepeng Cai & Qingge Xie

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  1. Junzheng Du
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  2. Huiyun Chang
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Correspondence to Huiyun Chang.

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Du, J., Chang, H., Cong, G. et al. Complete nucleotide sequence of a Chinese serotype Asia1 vaccine strain of foot-and-mouth disease virus. Virus Genes 35, 635–642 (2007). https://doi.org/10.1007/s11262-007-0126-8

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  • DOI: https://doi.org/10.1007/s11262-007-0126-8

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