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Characterization of an early gene (wsv477) from shrimp white spot syndrome virus (WSSV)

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Abstract

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. The viral early genes play a key role in DNA replication and virus proliferation. In this study, a WSSV gene (wsv477) encoding 208 amino acid peptides was characterized as an early gene. The temporal analysis showed that the wsv477 gene was first transcribed at 4 h post-infection, suggesting that it was an early gene. The wsv477 gene was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-WSV477). Western blot revealed that the wsv477 gene was expressed at 6 h post-infection in vivo. As indicated by GTP-binding assay, the WSV477 protein had GTP-binding activity.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (30525011 and 30330470) and Fujian Science and Technology Project.

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Authors and Affiliations

  1. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen, 361005, P. R. China

    Fang Han & Xiaobo Zhang

  2. School of Life Sciences, Xiamen University, Xiamen, 361005, P. R. China

    Jianyang Xu

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  1. Fang Han
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  2. Jianyang Xu
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  3. Xiaobo Zhang
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Correspondence to Xiaobo Zhang.

Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number DQ121373.

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Han, F., Xu, J. & Zhang, X. Characterization of an early gene (wsv477) from shrimp white spot syndrome virus (WSSV). Virus Genes 34, 193–198 (2007). https://doi.org/10.1007/s11262-006-0053-0

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