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The Complete Nucleotide Sequence and Genomic Organization of Citrus Leprosis Associated Virus, Cytoplasmatic type (CiLV-C)

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Abstract

The Citrus leprosis disease (CiL) is associated to a virus (CiLV) transmitted by Brevipalpus spp. mites (Acari: Tenuipalpidae). CiL is endemic in Brazil and its recently spreading to Central America represents a threat to citrus industry in the USA. Electron microscopy images show two forms of CiLV: a rare nuclear form, characterized by rod-shaped naked particle (CiLV-N) and a common cytoplasmic form (CiLV-C) associated with bacilliform-enveloped particle and cytoplasmic viroplasm. Due to this morphological feature, CiLV-C has been treated as Rhabdovirus-like. In this paper we present the complete nucleotide sequence and genomic organization of CiLV-C. It is a bipartite virus with sequence similarity to ssRNA positive plant virus. RNA1 encodes a putative replicase polyprotein and an ORF with no known function. RNA2 encodes 4 ORFs. pl5, p24 and p61 have no significant similarity to any known proteins and p32 encodes a protein with similarity to a viral movement protein. The CiLV-C sequences are associated with typical symptoms of CiL by RT-PCR. Phylogenetic analysis suggests that CiLV-C is probably a member of a new family of plant virus evolutionarily related to Tobamovirus.

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Acknowledgements

We thank Dr. Elliot Kitajima for the transmission electron microscopy, Robson F. Souza for the phylogenetic analysis advisement, Dr. Neusa Nogueira for the plant material symptomatic to Citrus Leprosis.

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Authors and Affiliations

  1. Alellyx Applied Genomics, Techno Park (Rod. Anhanguera Km 104), Rua James Clerk Maxwell 320, 13069-380, Campinas, SP, Brazil

    Renata C. Pascon, João Paulo Kitajima, Michèle C. Breton, Laura Assumpção, Christian Greggio, Almir S. Zanca, Vagner Katsumi Okura, Marcos C. Alegria, Maria E. Camargo, Giovana G.C. Silva, Jussara C. Cardozo, Marcelo A. Vallim, Sulamita F. Franco, Vitor H. Silva, Hamilton Jordão Junior, Fernanda Oliveira, Poliana F. Giachetto, Fernanda Ferrari, Carlos I. Aguilar-Vildoso, Fabrício J.B. Franchiscini, José M.F. Silva, Paulo Arruda, Jesus A. Ferro, Fernando Reinach & Ana Cláudia Rasera da Silva

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  1. Renata C. Pascon
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Correspondence to Ana Cláudia Rasera da Silva.

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Pascon, R.C., Kitajima, J.P., Breton, M.C. et al. The Complete Nucleotide Sequence and Genomic Organization of Citrus Leprosis Associated Virus, Cytoplasmatic type (CiLV-C). Virus Genes 32, 289–298 (2006). https://doi.org/10.1007/s11262-005-6913-1

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  • DOI: https://doi.org/10.1007/s11262-005-6913-1

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