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Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus

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Abstract

Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.

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Acknowledgements

This research was supported by the National Major Basic Research Project (“973”) of China (No: G2000016209 and 2003CB114202) and in part by the National Natural Science Foundation of China (No: 30370965).

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Authors and Affiliations

  1. State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China

    Weichun Chen, Zhaofei Li, Sainan Li, Lingling Li, Kai Yang & Yi Pang

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  1. Weichun Chen
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  2. Zhaofei Li
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  3. Sainan Li
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  4. Lingling Li
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  5. Kai Yang
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  6. Yi Pang
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Correspondence to Yi Pang.

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These authors contributed equally to the work.

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Chen, W., Li, Z., Li, S. et al. Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus. Virus Genes 32, 79–84 (2006). https://doi.org/10.1007/s11262-005-5848-x

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  • DOI: https://doi.org/10.1007/s11262-005-5848-x

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