Abstract
GST fusion proteins of the six gene products from RNAs 2,3 and 4 of the tenuivirus, Rice stripe virus (RSV), were used to study the nucleic acid binding activities in vitro. Three of the proteins, p3, pc3 and pc4, bound both single- and double-stranded cDNA of RSV RNA4 and also RNA3 transcribed from its cDNA clone, while p2, pc2-N (the N-terminal part of pc2) nor p4 bound the cDNA or RNA transcript. The binding activity of p3 is located in the carboxyl-terminus amino acid 154–194, which contains basic amino acid rich β-sheets. The acidic amino acid-rich amino-terminus (amino acids 1–100) of p3 did not have nucleic acid binding activity. The related analogous gene product of the tenuivirus, Rice hoja blanca virus, is a suppressor of gene silencing and the possibility of the nucleic acid binding ability of RSV p3 being associated with this property is discussed. The C-terminal part of the RSV nucleocapsid protein, which also contains a basic region, binds nucleic acids, which is consistent with its function. The central and C-terminal regions of pc4 bind nucleic acid. It has been suggested that this protein is a cell-to-cell movement protein and nucleic acid binding would be in accord with this function.
Similar content being viewed by others
References
S. Toriyama Y. Watanabe (1989) J Gen Virol 70 505–511
S. Toriyama (1986) Microbiol Sci 3 347–351
S. Toriyama M. Takahashi Y. Sano T. Shimizu (1994) J Gen Virol 75 3569–3579
M. Takahashi S. Toriyama C. Hamamatsu A. Ishihama ( 1993) J Gen Virol 74 769–773
Liang D., Qu Z., Ma X., and Hull R., accompanying paper submitted
Y. Hayano T. Kakutani T. Hayashi Y. Minobe (1990) Virology 177 372–374
T. Kakutani Y. Hayano T. Hayashi Y. Minobe ( 1990) J Gen Virol 71 1427–1432
T. Kakutani Y. Hayano T. Hayashi Y. Minobe (1991) J Gen Virol 72 465–468
Y. Zhu T. Hayakawa S. Toriyama M. Takahashi ( 1991) J Gen Virol 72 763–767
Z. Qu D. Liang G. Harper R. Hull (1997) Virus Genes 15 IssueID2 99–103
E. Bucher T. Sijen P. Haan ParticleDe R. Goldbach M. Prins (2003) J Virol 77 IssueID2 1329–1336
Y. Zhu T. Hayakawa S. Toriyama (1992) J Gen Virol 73 1309–1312
D. Baulcombe (2004) Nature 431 356–362
G. Moissiard O. Voinnet (2004) Mol Plant Pathol 5 71–82
B.M. Roth G.J. Pruss V.B. Vance ( 2004) Virus Res 102 97–108
R. Lu A. Folimonov M. Shintaku W.-X. Li B.W. Falk W.O. Dawson S.-W. Ding (2004) Proc Natl Acad Sci USA 101 15742–15747
L. Lakatos G. Szittya D. Silhavy J. Burguyan ( 2004) EMBO J 23 876–884
D.C. Baulcombe A. Molnár ( 2004) Trends Biochem Sci 29 279–281
K.D. Kasschau Z. Xie E. Allen C. Llave E.J. Chapman K.A. Krizan J.C. Carrington ( 2003) Dev Cell 4 205–217
J. Sukegawa G. Blobel (1993) Cell 72 29–38
Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., and Lipman D.J., Nucleic Acids Res 25, 3389–3402, 1997
J.D. Thompson T.J. Gibson F. Plewniak F. Jeanmougin D.G. Higgins (1997) Nucleic Acids Res 24 4876–4882
M. Gribskov R.R. Burgess J. Devereux (1986) Nucleic Acids Res 14 327–334
M.M.H. Storms R. Kormelink D. Peters J.W.M. Lent Particlevan R.W. Goldbach (1995) Virology 214 485–493
K.E. Richmond K. Chenault J.L. Sherwood T.L. German (1998) Virology 248 6–11
I.G. Maia F. Bernardi (1996) J Gen Virol 77 869–877
N.E. Yelina E.I. Savenkov A.G. Solovyev S.Y. Morozov J.P. Valkonen (2002) J Virol 76 12981–12991
U. Melcher (2000) J Gen Virol 81 257–266
T. Saito Y. Imai T. Meshi Y. Okada (1988) Virology 167 653–656
V. Citovski M.L. Wong A.L. Shaw B.V.V. Prasad P. Zambryski (1992) Plant Cell 4 397–411
T.A.M. Osman R.J. Hayes K.W. Buck (1992) J Gen Virol 73 223–227
D. Giesman-Cookmeyer S.A. Lommel (1993) Plant Cell 5 973–982
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liang, D., Ma, X., Qu, Z. et al. Nucleic Acid Binding Property of the Gene Products of Rice stripe virus. Virus Genes 31, 203–209 (2005). https://doi.org/10.1007/s11262-005-1797-7
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11262-005-1797-7