Skip to main content
SpringerLink
Log in

The Complete Genomic Sequence of Strain ROS/HUVLV-100, A Representative Russian Crimean Congo Hemorrhagic Fever Virus Strain

  • Original Article
  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

The complete genomic sequence (minus primer-generated ends) of the laboratory-adapted Crimean Congo hemorrhagic fever virus (CCHFV) strain ROS/HUVLV-100, isolated in 2003 from the blood of a deceased female from the Rostov region of southern European Russia, was determined by direct sequencing of overlapping reverse transcription/polymerase chain reaction amplified products. The size of the ROS/HUVLV-100 genome is 19.2 kilobases—individual genome segments are similar in size and sequence features to previously reported “Europe-1” group CCHFV strains. The low-passage ROS/HUVLV-100 strain is the first Russian Crimean Congo hemorrhagic fever virus isolate for which complete sequence information is available, and this work reports the first complete genomic CCHFV sequence determined from a single viral RNA preparation in the same laboratory.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. C.A. Whitehouse (2004) Antiviral Res. 64 145–160 Occurrence Handle15550268 Occurrence Handle1:CAS:528:DC%2BD2cXhtVSrtbzO Occurrence Handle10.1016/j.antiviral.2004.08.001

    Article  PubMed  CAS  Google Scholar 

  2. A.J. Sanchez M.J. Vincent S.T. Nichol (2002) J. Virol. 76 7263–7275 Occurrence Handle12072526 Occurrence Handle1:CAS:528:DC%2BD38XltVylsL8%3D Occurrence Handle10.1128/JVI.76.14.7263-7275.2002

    Article  PubMed  CAS  Google Scholar 

  3. J.E. Honig J.C. Osborne S.T. Nichol (2004) Virology 321 29–35 Occurrence Handle15033562 Occurrence Handle1:CAS:528:DC%2BD2cXisVSksL8%3D Occurrence Handle10.1016/j.virol.2003.09.042

    Article  PubMed  CAS  Google Scholar 

  4. E. Kinsella S.G. Martin A. Grolla M. Czub H. Feldmann R. Flick (2004) Virology 321 23–28 Occurrence Handle15033561 Occurrence Handle1:CAS:528:DC%2BD2cXisVSks7Y%3D Occurrence Handle10.1016/j.virol.2003.09.046

    Article  PubMed  CAS  Google Scholar 

  5. A. Bertolotti-Ciarlet J. Smith K. Strecker J. Paragas L.A. Altamura J.M. McFalls N. Frias-Staheli A. Garcia-Sastre C.S. Schmaljohn R.W. Doms (2005) J. Virol. 79 6152–6161 Occurrence Handle15858000 Occurrence Handle1:CAS:528:DC%2BD2MXkt1WitLY%3D Occurrence Handle10.1128/JVI.79.10.6152-6161.2005

    Article  PubMed  CAS  Google Scholar 

  6. R. Hewson A. Gmyl L. Gmyl S.E. Smirnova G. Karganova B. Jamil R. Hasan J. Chamberlain C. Clegg (2004) J. Gen. Virol. 85 3059–3070 Occurrence Handle15448369 Occurrence Handle1:CAS:528:DC%2BD2cXosV2gt74%3D Occurrence Handle10.1099/vir.0.80121-0

    Article  PubMed  CAS  Google Scholar 

  7. A.N. Lukashev (2005) J. Gen. Virol. 86 2333–2338 Occurrence Handle16033981 Occurrence Handle1:CAS:528:DC%2BD2MXpvVCgtb4%3D Occurrence Handle10.1099/vir.0.80974-0

    Article  PubMed  CAS  Google Scholar 

  8. J.D. Meissner, S.S. Seregin, S.V. Seregin, N.V. Yakimenko, O.I. Vyshemirskii, S.V. Netesov, V.S. Petrov, Arch. Virol. (in press)

  9. A. Papa E. Papadimitriou B. Bozovic A. Antoniadias (2005) J. Med. Virol. 75 466–469 Occurrence Handle15648072 Occurrence Handle1:CAS:528:DC%2BD2MXhs1Cjtro%3D Occurrence Handle10.1002/jmv.20290

    Article  PubMed  CAS  Google Scholar 

  10. S.V. Seregin E.I. Samokhvalov I.D. Petrova O.I. Vyshemirskii E.G. Samokhvalova D.K. Lvov V.V. Gutorov G.I. Tyunnikov S.N. Shchelkunov S.V. Netesov V.S. Petrov (2004) Virus Genes 28 187–193 Occurrence Handle14976418 Occurrence Handle1:CAS:528:DC%2BD2cXhtlynur4%3D Occurrence Handle10.1023/B:VIRU.0000016857.94136.89

    Article  PubMed  CAS  Google Scholar 

  11. L. Yashina I. Petrova S. Seregin O. Vyshemirskii D. Lvov V. Aristova J. Kuhn S. Morzunov V. Gutorov I. Kuzina G. Tyunnikov S. Netesov V. Petrov (2003) J. Gen. Virol. 84 1199–1206 Occurrence Handle12692285 Occurrence Handle1:CAS:528:DC%2BD3sXjsFCrsbs%3D Occurrence Handle10.1099/vir.0.18805-0

    Article  PubMed  CAS  Google Scholar 

  12. L. Yashina O. Vyshemirskii S. Seregin I. Petrova E. Samokhvalov D. Lvov V. Gutorov I. Kuzina G. Tyunnikov Y.-W. Tang S. Netesov V. Petrov (2003) J. Clin. Microbiol. 41 860–862 Occurrence Handle12574301 Occurrence Handle1:CAS:528:DC%2BD3sXhslGiurY%3D Occurrence Handle10.1128/JCM.41.2.860-862.2003

    Article  PubMed  CAS  Google Scholar 

  13. R. Hewson J. Chamberlain V. Mioulet G. Lloyd B. Jamil R. Hasan A. Gmyl L. Gmyl S.E. Smirnova A. Lukashev G. Karganova C. Clegg (2004) Virus Res. 102 185–189 Occurrence Handle15084400 Occurrence Handle1:CAS:528:DC%2BD2cXjtV2nsLc%3D Occurrence Handle10.1016/j.virusres.2003.12.035

    Article  PubMed  CAS  Google Scholar 

  14. M.P. Chumakov A.M. Butenko N.V. Shalunova L.I. Martyanova S.E. Smirnova Y. Bashkirtsev T.I. Zavodova S.G. Rubin E.A. Tkachenko V.Y. Karmisheva V.N. Reingold G.V. Popov A.P. Savinov (1968) Vopr. Virulsol. 13 377 Occurrence Handle1:STN:280:CCaD28zkt1c%3D

    CAS  Google Scholar 

  15. J.H. Kuhn S.V. Seregin S.P. Morzunov I.D. Petrova O.I. Vyshemirskii D.K. Lvov G.I. Tyunnikov V.V. Gutorov S.V. Netesov V.S. Petrov (2004) Arch. Virol. 149 2199–2213 Occurrence Handle15503207 Occurrence Handle1:CAS:528:DC%2BD2cXoslGisbo%3D Occurrence Handle10.1007/s00705-004-0354-3

    Article  PubMed  CAS  Google Scholar 

  16. K.S. Makarova L. Aravind E.V. Koonin (2000) Trends Biochem. Sci. 25 50–52 Occurrence Handle10664582 Occurrence Handle1:CAS:528:DC%2BD3cXht1Krsrw%3D Occurrence Handle10.1016/S0968-0004(99)01530-3

    Article  PubMed  CAS  Google Scholar 

  17. R. Muller O. Poch M. Delarue D.H.L. Bishop M. Bouloy (1994) J. Gen. Virol. 75 1345–1352 Occurrence Handle7515937 Occurrence Handle10.1099/0022-1317-75-6-1345

    Article  PubMed  Google Scholar 

  18. V.H. Aquino M.L. Moreli L.T. Moraes Figueiredo (2003) Arch. Virol. 148 19–28 Occurrence Handle12536293 Occurrence Handle1:CAS:528:DC%2BD3sXjvFGluw%3D%3D Occurrence Handle10.1007/s00705-002-0913-4

    Article  PubMed  CAS  Google Scholar 

  19. S. Kumar K. Tamura M. Nei (2004) Brief Boinform. 5 150–163 Occurrence Handle1:CAS:528:DC%2BD2cXntFGqu7s%3D Occurrence Handle10.1093/bib/5.2.150

    Article  CAS  Google Scholar 

  20. J. Felsenstein (1985) Evolution 39 783–791 Occurrence Handle10.2307/2408678

    Article  Google Scholar 

  21. J. Casals (1969) Proc. Soc. Exp. Biol. 131 233–236 Occurrence Handle1:STN:280:CCaC1cjltlc%3D

    CAS  Google Scholar 

  22. J.W. Hooper T. Larsen D.M. Custer C.S. Schmaljohn (2001) Virology 289 6–14 Occurrence Handle11601912 Occurrence Handle1:CAS:528:DC%2BD3MXnsFGisr0%3D Occurrence Handle10.1006/viro.2001.1133

    Article  PubMed  CAS  Google Scholar 

  23. R. Flick K. Flick H. Feldmann F. Elgh (2003) J. Virol. 77 5997–6006 Occurrence Handle12719591 Occurrence Handle1:CAS:528:DC%2BD3sXjs1OksLc%3D Occurrence Handle10.1128/JVI.77.10.5997-6006.2003

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by ISTC project #1291.2. We wish to thank Dr. J. Smith from USAMRIID, Fort Detrick, Maryland, USA for providing the SW-13 cell line.

Author information

Authors and Affiliations

  1. State Research Center of Virology and Biotechnology “VECTOR”, Koltsovo, Novosibirsk Region, 630559, Russia

    John D. Meissner, Sergey S. Seregin, Sergei V. Seregin, Oleg I. Vyshemirskii, Nina V. Yakimenko, Sergei V. Netesov & Vladimir S. Petrov

Authors
  1. John D. Meissner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sergey S. Seregin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sergei V. Seregin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Oleg I. Vyshemirskii
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nina V. Yakimenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sergei V. Netesov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Vladimir S. Petrov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to John D. Meissner.

Additional information

John D. Meissner is now at the Centers for Disease Control and Prevention, USA

Rights and permissions

Reprints and permissions

About this article

Cite this article

Meissner, J.D., Seregin, S.S., Seregin, S.V. et al. The Complete Genomic Sequence of Strain ROS/HUVLV-100, A Representative Russian Crimean Congo Hemorrhagic Fever Virus Strain. Virus Genes 33, 87–93 (2006). https://doi.org/10.1007/s11262-005-0041-9

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-005-0041-9

Keywords

Search

Navigation