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Characterization and Phylogenetic Analysis of Brazilian Chicken Anaemia Virus

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Abstract

Chicken anaemia virus (CAV) was detected by a Nested-PCR assay in field samples from different regions of Brazil. The 539 bp amplified fragments of vp1 gene from 44 field samples were sequenced and 10 new nucleotide sequences of CAV were observed. These sequences were phylogenetically analysed by Mega2 using neighbour joining distance methods with 1000 bootstrap replications. Phylogenetic analysis did not show correlation between CAV pathology pattern and genetic groups. The 10 nucleotide sequences of the Brazilian samples were also analysed together with 30 sequences of CAV strains previously described from other countries. The genetic variability observed was not related to the geographical distribution. Amino acid substitutions were detected at 9 positions of the Brazilian sequences and two of them had not been observed before, 65R replacing the Q residue and 98F replacing Y residue.

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Acknowledgements

We thank Brazilian chicken rearing companies for help in the collection of biological material. S. Simionatto received a scholarship from. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Financial support was provided by Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Pró-Reitoria de Pesquisa da Universidade Federal do Rio Grande do Sul (PROPESQ/UFRGS).

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Authors and Affiliations

  1. Laboratório de Virologia, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9090, Cep: 91540-000, Porto Alegre, RS, Brasil

    Simone Simionatto, Ana Paula Ravazzolo & Cláudio Wageck Canal

  2. Departmento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul -(UFRGS), Porto Alegre, RS, Brasil

    Carlos André da Veiga Lima-Rosa

  3. Laboratório de Biotecnoiogia e Bioinformática, Embrapa Soja, Londrina, PR, Brasil

    Eliseu Binneck

Authors
  1. Simone Simionatto
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  2. Carlos André da Veiga Lima-Rosa
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  3. Eliseu Binneck
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  4. Ana Paula Ravazzolo
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  5. Cláudio Wageck Canal
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Corresponding author

Correspondence to Cláudio Wageck Canal.

Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers AY855079 to AY855088.

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Simionatto, S., Lima-Rosa, C.A.d.V., Binneck, E. et al. Characterization and Phylogenetic Analysis of Brazilian Chicken Anaemia Virus. Virus Genes 33, 5–10 (2006). https://doi.org/10.1007/s11262-005-0033-9

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  • DOI: https://doi.org/10.1007/s11262-005-0033-9

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