Abstract
Virus DNA was isolated from horse faeces and cloned in a sequence-independent fashion. 268 clones were sequenced and 178140 nucleotides of sequence obtained. Statistical analysis suggests the library contains 17560 distinct clones derived from up to 233 different virus genomes. TBLASTX analysis showed that 32% of the clones had significant identity to GenBank entries. Of these 63% were viral; 20% bacterial; 7% archaeal; 6% eukarya; and 5% were related to mobile genetic elements. Fifty-two percent of the virus identities were with Siphoviridae; 26% unclassified phages; 17% Myoviridae; 4% Podoviridae; and one clone (2%) was a vertebrate Orthopoxvirus. Genes coding for predicted virus structural proteins, proteases, glycosidases and nucleic acid-binding proteins were common.
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D.A. Stahl D.J. Lane G.J. Olsen N.R. Pace (1984) Science 224 409–411 Occurrence Handle1:CAS:528:DyaL2cXksFSnt7o%3D
F.E. DeLong N.R. Pace (2001) Syst Biol 50 470–478 Occurrence Handle10.1080/106351501750435040 Occurrence Handle1:STN:280:DC%2BD38zntVOmsw%3D%3D Occurrence Handle12116647
F. Rodriguez-Valera FEMS Microbiol (2004) Lett 231 153–158
P.F. Lopez-Garcia F. Rodriguez-Valera C. Pedros-Alio D. Moreira (2001) Nature 409 603–607 Occurrence Handle10.1038/35054537 Occurrence Handle11214316
S.Y. Moon-van der Staay R. De Wachter D. Vaulot (2001) Nature 409 607–610 Occurrence Handle10.1038/35054541 Occurrence Handle1:CAS:528:DC%2BD3MXhtVygtbY%3D Occurrence Handle11214317
M. Breitbart P. Salamon B. Andresen J.M. Mahaffy A.M. Segall D. Mead F. Azam F. Rohwer (2002) Proc Natl Acad Sci USA 99 14250–14255
M. Breitbart I. Hewson B. Felts J.M. Mahaffy J. Nulton P. Salamon F.J. Rohwer (2003) Bacteriol 185 6220–6223
J.A. Fuhrman (1999) Nature 399 541–548 Occurrence Handle10.1038/21119 Occurrence Handle10376593
K.E. Wommack R.R. Colwell (2000) Microbiol Mol Biol Rev 64 69–114
R.W. Hendrix (2003) Curr Opin Microbiol 6 506–511
M.L. Pedulla M.E. Ford J.M. Houtz T. Karthikeyan C. Wadsworth J.A. Lewis D. Jacobs-Sera J. Falbo J. Gross N.R. Pannunzio W. Brucker V. Kumar J. Kandasamy L. Keenan S. Bardarov J. Kriakov JG. Lawrence WR. Jacobs RW. Hendrix GF. Hatfull (2003) Cell 113 171–182
D. Kristian C.S. Stewart H.J. Flint S.P. Shirazi-Beechey (2001) FEMS Microbiol Ecol 38 141–151
C.E. Shannon (1997) MD Comput 14 306–317
A. Chao (1984) Scandinavian J Stat. 11 783–791
S. Casjens (2003) Mol Microbiol 49 277–300 Occurrence Handle1:CAS:528:DC%2BD3sXlslGiu70%3D Occurrence Handle12886937
E.F. Boyd B.M. Davis B. Hochhut (2001) Trends Microbiol 9 137–144
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James Cann, A., Elizabeth Fandrich, S. & Heaphy, S. Analysis of the Virus Population Present in Equine Faeces Indicates the Presence of Hundreds of Uncharacterized Virus Genomes. Virus Genes 30, 151–156 (2005). https://doi.org/10.1007/s11262-004-5624-3
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DOI: https://doi.org/10.1007/s11262-004-5624-3