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Dietary lysine requirements of Colossoma macropomum (Cuvier, 1818) based on growth performance, hepatic and intestinal morphohistology and hematology

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Abstract

This study aimed to determine the dietary lysine requirements of juvenile Colossoma macropomum tambaqui based growth performance, hepatic and intestinal morphohistology and hematology. Tambaqui were fed with levels of 6.60, 9.72, 12.84, 15.96, 19.08, and 22.20 g/kg of total lysine and those who received 15.96 g/kg dietary lysine showed higher final weight (p= 0.001) and optimized feed conversion ratio (p= 0.001). Morphohistological modifications (presence of fat droplets) were present in livers of fish fed with low levels of lysine. In the morphohistology of the intestine, the diets influenced the density of the intestinal, cell layers, height and perimeters of the villi and acid mucin secretion by the goblet cells. In the proximal intestine, mucosa layer density was greater at the level of 15.96 g/kg (p= 0.001). In the middle intestine, height (p= 0.001) and perimeter (p= 0.001) of the villi were greater at low levels of lysine (respectively, 9.72 and 12.84 g/kg dietary lysine). No differences were found for the hemoglobin, hematocrit, erythrocytes, MCV, MCH, MCHC, thrombocytes and leukocytes differentiated of fish fed with experimental diets (p>0.05). The biochemical responses were affected by the diet, both in plasma concentration (protein and cholesterol; p<0.05), and in muscle (ash and moisture; p<0.05). In conclusion, the dietary requirement for lysine for tambaqui juveniles was estimated to be 15.4 - 15.6 g/kg of diet (5.7% –5.8% dietary protein).

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Data availability

The datasets in this study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was funded by the Amazonas State Research Support Foundation (FAPEAM) through the project PAPAC (Call 05/2019, process 062.00847/ 2019); Federal Institute of Education, Science and Technology of Amazonas (IFAM) throught the project PADCIT (Call 002/2020). ARSL is grateful for the PhD grant provided by Coordination for the Improvement of Higher Education Personnel (CAPES). ATO and WMS is CNPq research fellows.

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Authors and Affiliations

  1. Postgraduate Program in Animal Science and Fisheries Resources, Faculty of Agricultural Sciences – FAS, Federal University of Amazonas, Av. General Rodrigo Otávio Jordão Ramos, 6200, Manaus, Amazonas, 69067-005, Brazil

    Ariany Rabello da Silva Liebl

  2. Postgraduate Program in Infectious Diseases, Federal University of Espírito Santo, Campus Goiabeiras, Vitória, ES, Brazil

    Mirleide Araújo Cáo

  3. Postgraduate Program in Aquaculture, Nilton Lins University, Av. Nilton Lins, 3259, Manaus, Amazonas, 69058-030, Brazil

    Marcelo dos Santos Nascimento

  4. Animal Physiology Laboratory, Institute of Biological Sciences, Federal University of Amazonas, Av. Gal Rodrigo Otávio Jordão Ramos, 6200, Manaus, Amazonas, 69067-005, Brazil

    Philp Dalbert da Silva Castro

  5. Functional Morphology Laboratory, Institute of Biological Sciences, Federal University of Amazonas, Av. Gal Rodrigo Otávio Jordão Ramos, 6200, Manaus, Amazonas, 69067-005, Brazil

    Wallice Luiz Paxiúba Duncan & Adriano Teixeira de Oliveira

  6. Federal Institute of Eduction, Science and Technology of Amazonas, Campus Presidente Figueiredo, Av. Onça Pintada, 1308, Presidente Figueiredo, Amazonas, 69735-000, Brazil

    Jackson Pantoja-Lima

  7. Federal Institute of Education, Science and Technology of Amazonas, Av. 7 de setembro, 1975, Manaus, Amazonas, 69020-120, Brazil

    Paulo Henrique Rocha Aride

  8. Institute of Technology and Education Galileo da Amazônia, Av. Joaquim Nabuco, 1950, Manaus, Amazonas, 69020-030, Brazil

    Márcia Regina Fragoso Machado Bussons

  9. Department of Zootechnics - Block Z, Room 101, State University of Ponta Grossa, Avenida General Carlos Cavalcanti, 4748 - Uvaranas, 84030-900, Ponta Grossa, Paraná, Brazil

    Wilson Massamitu Furuya

  10. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences University of Messina, 98166, S.Agata-Messina, Italy

    Caterina Faggio

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  1. Ariany Rabello da Silva Liebl
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  2. Mirleide Araújo Cáo
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Contributions

ARSL, MAC, MSN, PDSC conceived the study. PHRA, MRFMB, WMF, ATO designed the study; ARSL, MAC, MSN undertook laboratorial analyses. ARSL, PHRA, CF, ATO drafted the paper with contributions from all other authors. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Adriano Teixeira de Oliveira.

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The experiment was developed in accordance with the rules of ethical principles for animal experimentation approved by the National Council for the Control of Animal Experimentation (CONCEA), subject to approval by the Ethics Commission on the Use of Animals (ECUA) of the Federal University of Amazonas under approval No. 005/2016 and ECUA of the Nilton Lins University under approval No. 003/2017. All experiments were conducted according to local and ARRIVE guidelines.

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da Silva Liebl, A.R., Cáo, M.A., dos Santos Nascimento, M. et al. Dietary lysine requirements of Colossoma macropomum (Cuvier, 1818) based on growth performance, hepatic and intestinal morphohistology and hematology. Vet Res Commun 46, 9–25 (2022). https://doi.org/10.1007/s11259-021-09872-6

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