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Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis

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Abstract

The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 μg/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7). The CMT score of both cytokines post infusion temporarily increased between days 0 and 1 and significantly decreased between days 7 and 14 compared to the preinfusion level. The SCC on day 14 after infusions of rbGM-CSF tended to be lower than that of the control group. The percentage of CD14+ cells increased on days 1 and 2 post infusion of rbGM-CSF. The percentage of CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentage of CD14+ cells decreased on days 0.25 and 1 post infusion of rbIL-8. No significant changes in the percentages of CD4+ and CD8+ cells in milk after infusion of rbIL-8 were evident during the experimental period, which suggested that rbIL-8 had little effect on the function of T cells in the mammary gland. These results indicated that rbGM-CSF and rbIL-8 decreased the CMT score by a different mechanism and may have a potential as therapeutic agents for subclinical mastitis.

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Abbreviations

cfu:

colony-forming units

CL:

chemiluminescence

CMT:

California mastitis test

PBS:

phosphate buffered saline

PMN:

polymorphonuclear neutrophils

rbGM-CSF:

recombinant bovine granulocyte-macrophage colony-stimulating factor

rbIL-8:

recombinant bovine interleukin-8

RLU:

relative light unit

SA:

Staphylococcus aureus

SCC:

somatic cell count

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Acknowledgements

This work was supported by Grant-in-Aid for Young Scientists (B) (No. 19780236, 25850215) from the Japan Society for the Promotion of Science (JSPS). The authors wish to acknowledge Dr. Petr Slama, Mendel University and Dr. Sota Kobayashi, National Institute of Animal Health, NARO, for their kind help and valuable advice. We are indebted to all the staff at Livestock Research Support Center, NARO Institute of Livestock and Grassland Science, Japan, for their cooperation in dealing with the animals. The authors would like to thank Enago (www.enago.jp) for the English language review.

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Authors and Affiliations

  1. Hokkaido Research Station, National Institute of Animal Health, NARO, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan

    Yoshio Kiku, Yuya Nagasawa, Atsushi Watanabe, Eiji Hata & Tomohito Hayashi

  2. National Institute of Animal Health, NARO, Tsukuba, Ibaraki, 305-0856, Japan

    Tomomi Ozawa, Hideyuki Takahashi & Shigeki Inumaru

  3. Japan Association for Techno-innovation in Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, 305-8601, Japan

    Hideyuki Takahashi

  4. NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, 305-0901, Japan

    Shiro Kushibiki & Hiroyuki Shingu

Authors
  1. Yoshio Kiku
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  2. Tomomi Ozawa
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  3. Hideyuki Takahashi
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  4. Shiro Kushibiki
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  5. Shigeki Inumaru
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  6. Hiroyuki Shingu
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  7. Yuya Nagasawa
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  8. Atsushi Watanabe
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  9. Eiji Hata
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  10. Tomohito Hayashi
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Correspondence to Tomohito Hayashi.

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Kiku, Y., Ozawa, T., Takahashi, H. et al. Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis. Vet Res Commun 41, 175–182 (2017). https://doi.org/10.1007/s11259-017-9684-y

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  • DOI: https://doi.org/10.1007/s11259-017-9684-y

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