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Expression kinetics of chicken β2-microglobulin and Class I MHC in vitro and in vivo during Marek’s disease viral infections

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Abstract

Marek’s disease virus (MDV) is a highly cell-associated herpesvirus that causes a disease in chickens characterized by tumor formation and immunosuppression. The changes of major histocompatibility complex (MHC) expression in different MDV-infected cells are not completely understood. In this study, we investigated the expression of the Class I MHC and β2-microglobulin (β2m) genes in response to MDV infection at different time points by real-time PCR. In both in vitro and in vivo, the expression levels of Class I MHC and β2m genes were upregulated during early MDV infections in comparison to control cells; We also found that the expression of Class I MHC gene was downregulated in BudR (5-bromo-2′-deoxyuridine)-treated MSB1 cells at 48 h and MDV-infected chicken embryo fibroblast cells (CEF) at 120 and 168 h post infection (hpi); Furthermore, compared to control groups, Class I MHC and β2m expression levels were downregulated in peripheral blood lymphocytes (PBLC) from MDV-infected chickens at 14 and 28 days post infection (dpi); Interestingly, both Class I MHC and β2m gene expression levels increased again in PBLC from MDV RB1B-infected chickens at 35 dpi, in which MDV was in the latent or transformed infection stages. In addition, Class I MHC expression was clearly decreased in MDV-infected CEF at 120 hpi although β2m expression was significantly increased. These changes in Class I MHC and β2m gene expression might provide more insights into host-virus interaction.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (31072135, 31272560), Major Basic Research of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Grant No. 12KJA23001) .

Conflict of interest

All authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No.12 East Wenhui Road, Yangzhou, Jiangsu, 225009, People’s Republic of China

    Chuan Yu, Aijian Qin, Xuming Hu, Kun Qian, Hongxia Shao & Wenjie Jin

  2. Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, People’s Republic of China

    Qiu Liu, Aijian Qin, Wencai Xu, Kun Qian, Hongxia Shao & Wenjie Jin

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  1. Chuan Yu
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  2. Qiu Liu
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  3. Aijian Qin
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  4. Xuming Hu
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  5. Wencai Xu
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  6. Kun Qian
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  7. Hongxia Shao
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  8. Wenjie Jin
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Correspondence to Aijian Qin.

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Yu, C., Liu, Q., Qin, A. et al. Expression kinetics of chicken β2-microglobulin and Class I MHC in vitro and in vivo during Marek’s disease viral infections. Vet Res Commun 37, 277–283 (2013). https://doi.org/10.1007/s11259-013-9572-z

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  • DOI: https://doi.org/10.1007/s11259-013-9572-z

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